Labshake search
Citations for Avidity :
51 - 100 of 128 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... biotinylated (BirA biotin-protein ligase bulk reaction kit [Avidity, USA]) and purified by size exclusion chromatography (Superdex 75 column [GE Healthcare] ...
-
bioRxiv - Immunology 2020Quote: ... Avitagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
-
bioRxiv - Immunology 2022Quote: ... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
-
bioRxiv - Biophysics 2020Quote: ... M2β-S1 used for the in vitro motility assays was biotinylated with BirA (10 µg/ml, Avidity) as described ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylation was performed using a BirA biotin-protein ligation kit (Avidity) on proteins produced with a C-terminal avidin tag sequence ...
-
bioRxiv - Microbiology 2020Quote: ... a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA) was used to biotinylate protein samples ...
-
bioRxiv - Biophysics 2022Quote: ... the nanodiscs were labeled with biotin using the BirA Enzyme (Avidity) following the protocol provided by the manufacture ...
-
bioRxiv - Immunology 2020Quote: ... were captured using mouse anti-AVI-tag mAb (Avidity LLC, Aurora, CO). In brief ...
-
bioRxiv - Immunology 2021Quote: Recombinant HA trimers were biotinylated by addition of biotin–protein ligase (Avidity). To generate HA tetramers ...
-
bioRxiv - Biophysics 2022Quote: ... coli.19 The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
-
bioRxiv - Immunology 2021Quote: ... for 10 min and excess streptavidin was quenched with 20 μM biotin (Avidity) for another 10 min ...
-
bioRxiv - Immunology 2022Quote: ... MHC multimers were diluted in PBS with 5.2 μM d-biotin (Avidity, Bio200) to 909 nM and incubated 20 min on ice ...
-
bioRxiv - Immunology 2023Quote: ... we extended the 14.4.4 scFV C-terminally with the BirA biotin ligase (Avidity) recognition site (GLNDIFEAQKIEWHE ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylation of monomer was carried out using BirA biotin-protein ligase kit (Avidity) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... Overnight biotinylation reactions were performed using the BirA Biotin-Protein Ligase Kit (Avidity) at 4℃ in 1x BiomixA ...
-
bioRxiv - Biochemistry 2024Quote: ... The HKU1 RBDs were biotinylated using the BirA biotin ligase reaction kit (Avidity) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... Tetramers with an AviTag were biotinylated with the BirA biotin-ligase kit (Avidity) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The RBD with a BirA tag was biotinylated using a biotinylation kit (Avidity, LLC) and was immobilized through the Biotin CAPture Reagent ...
-
bioRxiv - Biochemistry 2022Quote: ... the purified RBDs were biotinylated using the BirA biotin-protein ligase reaction kit (Avidity). The biotinylated proteins were re-purified and concentrated as described above ...
-
bioRxiv - Genetics 2021Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity).
-
Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... Excess streptavidin was blocked with two 40 s injections of 500 µM biotin (Avidity) and the sensor was conditioned with at least 8 injections of running buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... the proteins were biotinylated using the BirA biotin-protein ligase reaction kit (Avidity, #BirA500). Biotinylation of the NiV protein probes was confirmed by biolayer interferometry by testing the ability of the biotinylated protein to bind to streptavidin sensors ...
-
Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 µM biotin (Avidity). Before injections of soluble 1G4 or A6 αβTCR (51 kDa) ...
-
bioRxiv - Immunology 2021Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 µM biotin (Avidity). Before injections of soluble 1G4 or A6 αβTCR (51 kDa) ...
-
bioRxiv - Immunology 2020Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity). Before TCR injections ...
-
bioRxiv - Microbiology 2021Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity). Before RBD injections ...
-
bioRxiv - Immunology 2020Quote: ... purified PR8-HA and S proteins were biotinylated using BirA biotin-protein ligase (Avidity). Biotinylated PR8-HA and S proteins were fluorescently labelled by the sequential addition of streptavidin-conjugated to phycoerythrin (PE ...
-
bioRxiv - Immunology 2021Quote: ... each FcγR was biotinylated using a BirA biotin-protein ligase bulk reaction kit (Avidity) according to the protocol of the manufacturer ...
-
bioRxiv - Immunology 2023Quote: BSP-tagged proteins were biotinylated using the BirA biotin-ligase bulk reaction kit (Avidity), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity). Before antibody injections ...
-
bioRxiv - Immunology 2024Quote: ... biotinylation with BirA biotin-protein ligase standard reaction kit (Avidity, 318 LLC-Aurora, Colorado), and purification using size-exclusion column (Waters ...
-
bioRxiv - Immunology 2024Quote: ... HLA-DR monomers were biotinylated overnight at 4°C using BirA biotin ligase (Avidity) and purified by size exclusion chromatography using Superdex 200 size exclusion column (AKTA ...
-
bioRxiv - Biophysics 2021Quote: ... coli BL21(DE3) cells harboring pBirAcm encoding the biotin ligase (Avidity, LCC, Aurora, Colorado, USA). For over-expression cells over-night cultures were diluted 1:100 in fresh LB medium supplemented with 20 mg/l Biotin ...
-
bioRxiv - Immunology 2022Quote: ... VH F6 was biotinylated with BirA biotin-protein ligase standard reaction kit (BirA500, Avidity, USA). Streptavidin biosensors (ForteBio ...
-
bioRxiv - Microbiology 2021Quote: ... TGEV and CCoV-HuPn-2018 were biotinylated using BirA biotin-protein ligase standard reaction kit (Avidity) following manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
-
bioRxiv - Immunology 2020Quote: ... The RBD protein was biotinylated with the BirA biotin-protein ligase kit (Avidity LLC, Colorado, USA) using the manufacturer’s protocol.
-
bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
-
bioRxiv - Immunology 2020Quote: ... BirA biotinylation of S proteins was conducted using BirA biotin-protein ligase bulk reaction kit (Avidity). Coronavirus S proteins with Avi-tags were concentrated to 7-9 mg/ml using 100K Amicon tubes in TBS ...
-
bioRxiv - Biophysics 2023Quote: ... For in in vivo biotinylation on the Avi-tag the plasmid pBirA (Avidity Nanomedicines, La Jolla, CA) was cotransformed and the cell culture procedure was adapted according to Avidity’s in vivo biotinylation protocol.
-
bioRxiv - Biophysics 2021Quote: Monomeric H57-scFV featuring a BirA recognition site was site-specifically biotinylated using a biotin ligase (Avidity) at 30 °C ...
-
bioRxiv - Microbiology 2021Quote: ... The samples were then biotinylated in vivo using a BirA biotin-protein ligase standard reaction kit (Avidity). Following the kit instructions ...
-
bioRxiv - Immunology 2022Quote: ... and HA were prepared individually and mixed together after multimerization with 5uM free D-biotin (Avidity LLC) to minimize potential cross-reactivity between probes ...
-
bioRxiv - Immunology 2021Quote: ... and HA were prepared individually and mixed together after multimerization with 5uM free D-biotin (Avidity LLC) to minimize potential cross-reactivity between probes ...
-
bioRxiv - Immunology 2023Quote: ... Antigen probes were prepared individually and mixed together after multimerization with 5μM free D-biotin (Avidity LLC) to minimize potential cross-reactivity between probes ...
-
bioRxiv - Biophysics 2019Quote: ... was C-terminally equipped with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation via the BirA ligase (Avidity) followed by a 3C protease cleavable (LEVLFQGP ...
-
bioRxiv - Biochemistry 2021Quote: ... Purified proteins with an avi-tag were biotinylated by using BirA following the BirA500 kit’s protocol (Avidity, BirA500). Biotinylation was confirmed by performing a Coomassie gel shift assay according to Fairhead and Howarth ...
-
bioRxiv - Microbiology 2021Quote: ... the probe was generated by biotinylation of the GPΔmuc-WL2P2-1TD0-Avi trimer using biotin ligase BirA according to the manufacturer’s instructions (Avidity). Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare) ...
-
bioRxiv - Immunology 2022Quote: ... AviTagged tri-S and RBD proteins were biotinylated using BirA biotin-protein ligase bulk reaction kit (Avidity, LLC) or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2020Quote: ... Avi-tagged HAs were expressed and purified as described above and biotinylated using the Biotin ligase kit (Avidity) according to the manufacturer’s protocol ...