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Citations for KeraFAST :
101 - 139 of 139 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the samples were examined by Western blot with anti-puromycin antibody (KeraFAST, Cat # EQ0001, 1:1000). For the determination of histone acetylation level ...
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bioRxiv - Microbiology 2020Quote: ... Immune staining was performed by incubation with 1:3000 anti-VSV-M [23H12] monoclonal antibody (Kerafast) on the infected cells for 90 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies used were anti-puromycin (3RH11) antibody (1:1000, Kerafast, Boston, USA, cat. no. EQ0001), anti-eIF2alpha (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies include the following: rabbit-anti-eEF1A ([1:10,000 - IB], ED7001, Kerafast Inc., Boston, MA), mouse-anti-FLAG M2 ([1:2000-1:5000 - IB] ...
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bioRxiv - Microbiology 2021Quote: ... Samples were loaded onto either 6% or 10% TBE-Urea Gel (Thermo Fischer Scientific) along with a biotinylated sRNA ladder (Kerafast). Gels were run in 1x TBE buffer at 180V for 60 min (6% gels ...
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bioRxiv - Microbiology 2021Quote: RNA samples were loaded onto either 6% or 10% TBE-Urea Gel (Thermo Fischer Scientific) with a biotinylated RNA ladder (Kerafast). Gels were run in 1x TBE buffer at 180V for 60 minutes (6% gels ...
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bioRxiv - Immunology 2022Quote: VSV pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously.66 BHK-21/WI-2 cells were plated into a 6 well plate ...
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bioRxiv - Immunology 2022Quote: VSV vectors pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously [51] ...
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bioRxiv - Developmental Biology 2021Quote: Western blot analyses were performed according to standard protocols with mouse anti-puromycin (Kerafast, 3RH11; 1/500) or mouse anti-H3 (Diagenode ...
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bioRxiv - Molecular Biology 2020Quote: P53-(1-73) variants were then transformed into BL21(DE3) low background strain (LOBSTR, Kerafast, Boston, Massachusetts) chemically competent cells and selected for with LB agar plates with 50 µg/mL Kanamycin and 1%(w/v ...
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bioRxiv - Microbiology 2020Quote: ... and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene ...
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bioRxiv - Bioengineering 2022Quote: ... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
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bioRxiv - Neuroscience 2020Quote: ... and protein synthesis level was determined by Western blot using anti-puromycin antibody (KeraFAST, Cat#EQ0001, 1:1000). The combined intensity of proteins ranging from 20 to 200 kDa was quantified and normalized to the level of β-actin.
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bioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: mouse anti-Puromycin (Kerafast Cat# EQ0001, RRID:AB_2620162, 1:3000), rabbit anti-RpS12 (Proteintech Cat#16490-1-AP ...
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bioRxiv - Plant Biology 2023Quote: ... then incubated with a 1:100 dilution of LM20 or LM19 primary antibody (Kerafast, ELD003 and ELD001 respectively) (Verhertbruggen et al. ...
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bioRxiv - Immunology 2021Quote: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011), African Green monkey kidney-derived Vero E6 cell line (ATCC ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight on a rotating wheel at 4 °C in presence of 1 μg of S9.6 antibody (Kerafast) and 20 μl of protein G sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells were purchased from Kerafast (Boston, MA, USA) and cultured in D5+ media ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with a spike-expressing construct using 1 mg/mL PEI and then infected with VSV-G pseudotyped ΔG-luciferase (G*ΔG-luciferase, Kerafast) one day post-transfection ...
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bioRxiv - Microbiology 2022Quote: Baby hamster kidney cells (BHK-21/WI-2, Kerafast # EH1011, Boston, MA, USA) and African Green Monkey Cell Line (VeroE6 ...
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bioRxiv - Neuroscience 2020Quote: Detection of newly synthesized proteins by proximity ligation was performed as previously described6 ssing a mouse anti-puromycin (Kerafast, 1:500) antibody in combination with rabbit anti-beta actin (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Each section was placed individually in a sealed custom small plastic packet containing 100 µL of a primary antibody solution with a 1:500 dilution of mouse anti-puromycin antibody (Kerafast EQ0001), 1:300 rabbit anti-IBA1 (Thermo ab5076) ...
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bioRxiv - Microbiology 2020Quote: ... The denatured TCA-treated proteins were subjected to immunoblot analysis for VSV M and EBOV GP protein levels using anti-VSV M (1:2500, Kerafast, EB0011) and anti-EBOV GP (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... After 1.5 h at 37°C cells were washed with PBS and incubated with DMEM containing 1 μg/ml anti-VSV-G antibody (Kerafast, EB0010) for 24 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... – a heterogenous mixture with most chains between 200 and 1300 phosphate units and a purity of <1% monophosphate] was purchased from Kerafast (Boston, MA). TOC1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was replenished with DMEM+10%FBS containing a 1:20,000 dilution of an anti-VSV-G antibody 8G5F11 (Kerafast Cat. No EB0010), which reduces any possible background from residual VSV-G ...
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bioRxiv - Plant Biology 2023Quote: ... Sections were treated with blocking solution containing 3% (w/v) skim milk in PBS for 30 min and then with LM5 antibody (Kerafast, USA) diluted 5-fold in blocking solution for 1 h ...
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bioRxiv - Neuroscience 2019Quote: Detection of newly synthesized proteins by proximity ligation was carried out using anti-puromycin antibodies (mouse anti-Puromycin from Kerafast, 1:500-1000) in combination with protein-specific antibodies (rabbit anti-RapGEF4 from Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was incubated with a dilution series of mouse serum (dilutions as indicated) and anti-VSV-G (Kerafast; 1 μg/mL) antibody for 30 minutes at room temperature and added to the HEK-Blue 293 hACE2-TMPRSS2 cells ...
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bioRxiv - Microbiology 2022Quote: ... serum-containing medium was replaced by serum-free DMEM and cells were inoculated with MOI 3 of VSVΔG*/Luc-G (Kerafast, Boston, MA, USA) for the production of luciferase-expressing pseudotypes ...
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bioRxiv - Immunology 2021Quote: ... and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Microbiology 2020Quote: ... and were infected 2 days post-transfection with >107 infectious units/well of VSVΔG-GFP (Kerafast, (22)) ...
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bioRxiv - Immunology 2021Quote: ... 2021) and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Immunology 2020Quote: ... HEK293T/17 monolayers transfected to express SARS-CoV-2 S truncated of the C-terminal cytoplasmic 19 amino acids (SARS-CoV-2-S-CΔ19) were inoculated with VSVΔG-GFP vector (rescued from pVSVΔG-GFP plasmid expression vector; Kerafast Inc.). After incubation for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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bioRxiv - Microbiology 2020Quote: ... Cells were rinsed with PBS one hour after infection and replace with complete media supplemented with 2 microliters of mouse hybridoma supernatant containing anti-VSV-G antibody I1 (Kerafast) to neutralize input virus ...
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bioRxiv - Molecular Biology 2023Quote: Reporter gene Renilla luciferase was cloned into the multiple cloning site MCS-2 of plasmid pVSV-ΔG-N/P-MCS2-2.6 (Kerafast, Boston Massachusetts), which encodes the positive-sense RNA of a replication-defective recombinant vesicular stomatitis virus (rVSV ...
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bioRxiv - Microbiology 2021Quote: ... Pseudoviruses were generated in BHK-21/WI-2 cells using a pseudotyped ΔG-GFP (G*ΔG-GFP) rVSV (Kerafast, Boston, MA, USA) in addition to spike sequences cloned into the paT7- Spike plasmid as described previously (21) ...