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Citations for KeraFAST :
1 - 50 of 139 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... 5 μg of S9.6 antibody (1:100 dilution, ENH001, Kerafast) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked with TBST-5% non-fat milk at room temperature for 1 h and incubated with 1:1000 s9.6 (Kerafast, ENH001) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg chromatin was used for immunoprecipitation (IP) reactions in 100 µL aliquots with 5 µg S9.6 antibody (Kerafast), as per the kit protocol ...
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bioRxiv - Neuroscience 2020Quote: ... hiPSCs were seeded onto freshly coated 6-well plates 3 days prior to transduction with premade LV-CAG-eGFP lentivirus (Kerafast FCT149, 1 × 108 CFU/ml). Polybrene (2 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA-RNA hybrid [S9.6] antibody (Kerafast, 1:1000) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... cells were infected at a multiplicity of infection of 3-5 with rVSV-ΔG pseudovirus bearing a luciferase gene (Kerafast) for 2 hours at 37°C and then washed 6 times with 1X PBS ...
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bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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bioRxiv - Biochemistry 2019Quote: ... biotinylated medium chain polyP (5-20 ug/uL, Kerafast) or 5’-biotinylated single-strand DNA (5 ug/uL ...
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bioRxiv - Cell Biology 2021Quote: ... were immunoprecipitated using 5 μg of S9.6 antibody (Kerafast) and 20 μl of Protein G Dynabeads (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-puromycin (IB, 1:1000, IF 1:3500, Kerafast,), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA–RNA hybrid [S9.6] antibody (Kerafast) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were gently washed three times 2-hour post-infection and incubated in DMEM containing 1:1000 dilution of anti-VSV-G antibody (Kerafast: 8G5F11) to eliminate residual ΔG-VSV particles at 37°C ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were subsequently briefly rinsed thrice with 2% BSA/0.05% Tween (in PBS) and incubated with mouse anti-DNA– RNA hybrid S9.6 (Kerafast, ENH001; 1:100) and rabbit anti-nucleolin (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.0/140 mM NaCl/0.05% Triton X-100) and 5 μg of the S9.6 antibody (Kerafast, 1mg/ml) or 5 μg of the BrdU antibody (#555627 ...
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bioRxiv - Microbiology 2021Quote: ... The blocking solution was removed and replaced with fresh TBST containing 5% (w/v) milk and S9.6 anti-DNA-RNA Hybrid Antibody (Kerafast) at 1μg/ml and incubated at 4°C overnight ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was generated by nucleofection of BHK cells (maintained in DMEM/F12 with 10%FBS and 5%TPB) with Spike-expressing plasmid followed by transduction with G-pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast) 18-24 hours later ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2022Quote: ... 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex, Cat #: GTX135591, 1:1,000 dilution) (mouse anti-VSV-M, Kerafast, Cat #: EB0011, 1:100,000 dilution), prepared in WB blocking buffer (5% skim milk (Bio-Rad ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:3500 mouse Puromycin (Kerafast), 1:1000 rabbit β-Tubulin 9F3 (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... homopropargyl glycine (1 mM; Kerafast), and appropriate drug for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... respectively (diluted 1:50, Kerafast) followed by Alexa Fluor 594 anti-rat IgG (diluted 1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-tdTomato (1:500, Kerafast EST203). Anti-cathepsin L antibody (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Toxoplasma α-Centrin-1 (Kerafast company) 1:2,000 ...
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bioRxiv - Plant Biology 2021Quote: ... anti-ARP6 (1:200, Kerafast, EGA929), anti-DET3 (1:10000 ...
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bioRxiv - Biochemistry 2022Quote: ... α-puromycin (dilution 1:10,000; Kerafast EQ0001), and α-β-actin (dilution 1:30,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-puromycin (1:2000, Kerafast, 3RH11), anti-Rpl11 (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... tdTomato [16D7] (#EST203, 1:200, Kerafast), RFP (#600-401-379 ...
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bioRxiv - Microbiology 2023Quote: ... C57BL/6 wild type and iNOS knockout mutant (Kerafast) cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s medium (DMEM ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the following primary antibodies overnight at 4°C for S9.6-HIV-1-IN_PLA: mouse anti-DNA–RNA hybrid S9.6 (1:250; Kerafast, ENH001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-Puromycin (Kerafast, 1:500-1000), guinea pig anti-VGLUT1 (Synaptic Systems ...
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bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse anti-PMY (Kerafast, 1:1000) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mCherry antibody (1:500, Kerafast) (immunohistochemistry) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-mCherry (1:500, Kerafast, EMU106); rat anti-TFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-TFP (1:500, Kerafast, EMU104), guinea pig anti-TagBFP (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... and VSV-M (1:2500, Kerafast, EB0011).
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bioRxiv - Developmental Biology 2019Quote: ... anti-COL1A1 (LF-68, Kerafast; 1:100), AF568-conjugated goat-anti-rabbit IgG (A11011 ...
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bioRxiv - Developmental Biology 2021Quote: ... LM19 and LM20 (Kerafast, diluted 1:10) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-puromycin (1:2000 dilution, Kerafast, 3RH11), anti-Rpl11 (1:300 dilution ...
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bioRxiv - Genetics 2022Quote: ... SPP1 at 1:300 (ENH094-FP, Kerafast), SP7/OSX at 1:100 (Ab22552 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-S9.6 (mouse-monoclonal, Kerafast; 1:200), anti-PCNA (rabbit-polyclonal ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (Bigalke et al ...
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bioRxiv - Genomics 2019Quote: ... Antibodies used for immunostaining include the following: primary antibodies (anti-γH2A.X, Cell Signaling, 1:500; anti-FMRP, Cell signaling, 1:200; S9.6, Kerafast, 1:500 for lymphoblasts and 1:120 for fibroblasts ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (NEC construct absent of the UL34 C-terminal transmembrane helix)14 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and/or VSV-M (Kerafast; 23H12; 1:5,000) expression on 10% Bis-Tris PAGE gel (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... and Mouse Anti-Puromycin(PMY) (Kerafast, 1:1000) at room temperature ...