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Citations for PolyScience :
51 - 94 of 94 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cells were washed with PBS buffer and incubated with Uvitex2B (Polyscience Inc, Warrington, PA, USA) for 1 hour at room temperature and subsequently ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors were then transiently transfected into the HEK-293F cells using polyethyleneimine (Polyscience). After 3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with Strep-HA-VCF1 expression plasmids using PEI transfection reagent (Polyscience Inc). After 30 h ...
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Immunology 2022Quote: ... The expression vectors were transiently transfected into HEK-293F cell lines (Clover Biopharma) using PEI (Polyscience) and grown in OPM-293 CD05 medium (OPM ...
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bioRxiv - Immunology 2020Quote: ... The expression vectors were transient transfected into HEK-293F cell lines (Clover Biopharma) using PEI (Polyscience) and grown in OPM-293 CD05 medium (OPM ...
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bioRxiv - Molecular Biology 2024Quote: ... cell pellets were washed twice with sterile PBS and subjected to LR White (Polyscience, Inc., USA) embedding ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T cells were transfected with were performed using PolyEthylene Imine (PEI)50 (PEI linear, Mr 25,000, Polyscience). Neuro2A transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 800 ng of mHilpda_mCherry plasmid complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Microbiology 2021Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Plant Biology 2022Quote: ... 24 h after seeding cells were transfected using a polyethylenimine-based (PEI, linear, MW: 25 kDa, Polyscience) method ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were transfected with expression and packaging plasmids following standard calcium phosphate or polyethylenimine (Polyscience, 23966) protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... En bloc staining was done in the dark with 1% UA (cat# 21447, Polyscience Europe GmbH) in water at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... transfected to L cells together with a piggyBac transposase vector pCAG-PBorf using Transporter 5 transfection reagent (Polyscience), and selected in 1mg/ml Zeocin (Invivogen ...
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bioRxiv - Biochemistry 2020Quote: ... Next day cells were transfected with 750 ng of Hilpda_sYFP2 complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum free medium ...
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bioRxiv - Microbiology 2022Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then transfected with either Reelin-HA or control plasmid using 1.0 mg/ml polyethyleneimine MAX (Polyscience). After 2 days ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were seeded into 10 cm culture dishes and transfected the next day using polyethyleneimine (PolyScience, Niles, IL) with the following plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... The minigene constructs (c.21-94G>A or WT) were transfected into cultured 293T cells using PEI reagent (Polyscience). Cells were harvested 48 h after transfection and total RNA was extracted using TRIzol following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Cell Biology 2019Quote: ... along with a 1 µl suspension containing M9 and 20 µm diameter poly-styrene microspheres (Polyscience, Warrington, PA). The preparation was then covered with another coverslip and sealed using vaseline ...
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bioRxiv - Microbiology 2023Quote: ... DNA (1 µg/ml final transfection volume) was added followed by addition of linear polyethylenimine (PEI25k, Polyscience, Inc.) (1mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Center) were co-transfected into Lenti-X 293T cells using polyethyleneimine (Polyscience). Virus-containing media were collected at 48 or 72 h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids containing shRNA or corresponding empty vector were cotransfected with lentivirus packaging plasmids (pLP1, pLP2, and pLP/VSVG) into HEK293T cells using linear polyethylenimine (23966, Polyscience) transfection method ...
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bioRxiv - Microbiology 2021Quote: Virus stocks were obtained by transfecting of HEK 293T cells with proviral DNA in the presence or absence of pSVCMV-VSV-G using polyethylenimine (PEI; Polyscience, #23966). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: pCSII-EF-MCS- plasmids expressing Flag-tagged FANCJ WT and AALA mutant were transfected into HEK 293T (shown in Figure 1C and 5A) or FANCJ-KO HeLa cells (shown in Figure 7B) using poly-ethylenimine (PEI, Polyscience, Inc.). At 48 hr post-transfection ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were cotransfected with pcDNA3.1-Env clone and Env-deficient HIV-1 backbone vector (pSG3△Env) using PEI transfection reagent (PolyScience). Pseudovirus-containing supernatant was harvested 48h post-transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... for 24 hours after which transfection took place using 187.5 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... and 5 μg of pITR vector (S2/S12/RBD/SRBD/GFP) were transfected into a 10-cm diameter dish with HEK-293T cells by Polyethylenimine (PEI) (PolyScience, Niles, USA). The HEK-293T cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... and AAV rep and cap plasmid for the construction PHP.eB capsid were co-transfected into HEK293T cells using polyethyleneimine (catalogue no. 24765; Polyscience, Inc., Warrington, PA). The cells and culture media were separately harvested 72 h after transfection ...
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bioRxiv - Immunology 2019Quote: ... for IgG was mixed with 25 mL Opti-MEM containing 2,250 µg polyethylene imine MAX (MW 40,000; Polyscience - 24765-1). After incubation for 20 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... This plasmid was then transfected into immortalized STING-knockout MEFs (Mukai et al., 2016) with PEI MAX (24765-1, Polyscience). Twenty-four hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a total of 800 ng of plasmids encoding both Target-AID and gRNA were combined with 2.5 µL of 1 mg/mL PEI MAX (Polyscience #24765) and 200 µL of 1x PBS and applied to each well after 5–10 min incubation at room temperature.
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bioRxiv - Bioengineering 2023Quote: ... cells were transiently transfected at a concentration of 140 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... For the retrograde tracing of lung-innervating VG neurons mice received a volume of 40 µl Fast Blue solution (350 µM and 1% DMSO in 0.9% saline, 73819-41-7, Polyscience Inc) or 40 µl DiD solution (0.5 m/ml in 0.9% saline ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNAs were introduced into Lenti-X 293T or HEK293T cells using either polyethylenimine hydrochloride (PEI) (49553-93-7, Polyscience, Niles, IL, USA) or TransIT®-293 Transfection Reagent (MIR2700 ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: ... or 100 pmol/mL of SC-siRNA and linear polyethylenimine (PEI) with a molecular weight of 25 kDa (23966-1; Polyscience, USA) in a 1:3 ratio (μg of siRNA ...
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bioRxiv - Molecular Biology 2024Quote: 1.6 µg of expression plasmid DNA in 100 µl of 150 mM NaCl were mixed with 100 µl of 150 mM NaCl supplemented with 7 µl of 1 mg/ml PEI (polyethylenimine ‘MAX’ Polyscience, Inc.). After 30 min incubation at a room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... The lentiviral vector was cotransfected into HEK293T cells (RCB2202, RIKEN, Japan) with psPAX2 and pCMV-VSV-G-RSV-Rev using Polyethylenimine Max (Polyscience Inc., Warrington, PA, USA). Culture supernatants containing the virus were collected at 48 hours after transfection ...
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bioRxiv - Genetics 2023Quote: ... 15 μg DNA was incubated in 600 μL Opti-MEM and 60 μL of 1 mg/mL linear PEI (Polyscience, 23966-2) for 15 minutes at room temperature before adding to cells dropwise ...
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bioRxiv - Neuroscience 2023Quote: ... and the pHGT1-Adeno1 helper plasmid carrying adenoviral genes (kindly provided by C. Cepko, Harvard Medical School, Boston, USA) using PEIMAX (Polyscience, #POL24765-1). Plasmids were mixed in 98 mL DMEM (Thermo Fischer ...