Labshake search
Citations for PolyScience :
1 - 50 of 94 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
-
bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected using PEI (23966-1, Polyscience, Warrington, PA) with the pLentiCRISPRv2-LoxPv1 gRNA containing plasmid ...
-
bioRxiv - Neuroscience 2020Quote: ... Transfection of HEK293T cells was carried out using 1 mg/ml Polyethyleneimine MAX (Polyscience). Mycoplasma contamination was monitored by DAPI staining.
-
bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto assay plates and transfected using polyethyleneimine (PEI, Polyscience, 1 mg/mL). Details on the conditions for each experiment can be found in Supplementary Table 2 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
-
bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...
-
bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
-
bioRxiv - Immunology 2023Quote: ... and all plasmid constructs used throughout the study were transfected into cells using PEI transfection reagent (Polyscience, Cat; 24765-1) at a concentration of 1□µg/ml ...
-
bioRxiv - Immunology 2022Quote: The heavy and light chain plasmids were then co-transfected into HEK293 cells us-ing PEI MAX® (Cat: 24765-1, PolyScience). The production supernatant was harvested af-ter 14 days post-transfection and purified using AKTA equipped with a PpL affinity col-umn (Cat ...
-
bioRxiv - Cell Biology 2019Quote: ... and pCMV-VSV-G-RSV-Rev were co-transfected into Lenti-X 293T cells using polyethylenimine (no. 24765-1, Polyscience Inc., Warrington, PA). To prepare the retrovirus ...
-
bioRxiv - Cell Biology 2021Quote: ... and pCMV-VSV-G-RSV-Rev were co-transfected into Lenti-X 293T cells using polyethylenimine (no. 24765-1, Polyscience Inc., Warrington, PA). The infected cells were selected with media containing the following antibiotics ...
-
bioRxiv - Biophysics 2024Quote: ... and a fresh 1:10,000 dilution of 1 µm beads (Polybead® Microspheres 1 µm, Polyscience, Inc) in medium was added to the sample ...
-
bioRxiv - Biophysics 2023Quote: ... Previously 1 μm beads (Polybead® Microspheres 1 μm, Polyscience, Inc) were added in 1:10,000 dilution to the medium ...
-
bioRxiv - Microbiology 2023Quote: HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
-
bioRxiv - Neuroscience 2022Quote: Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells growing in 6-well plates (2.5×105 cells/well) were transfected using linear PEI ~25000 Da (Polyscience) as described previously24 Cells were transfected using a ratio μg DNA/μl PEI of 1/15 and the DNA/PEI mix was incubated for 20 min at room temperature before adding to the cells ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were mounted in Aqua polymount (Polyscience) and coverslips were sealed with nail-polish to prevent hardening of the mounting media ...
-
bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL polyethylenimine (PEI, Polyscience) in PBS was used for the transfection at a ratio of 3:1 (μL:μg ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were transfected using polyethylenimine (PEI; Polyscience, 24765) dissolved in PBS to 1 mg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were fixed in 2 % paraformaldehyde (Polyscience; Hirschberg an der Bergstrasse ...
-
bioRxiv - Immunology 2021Quote: ... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected using polyethyleneimine MAX (PEI Max) (Polyscience). The amount of the plasmids and PEI Max were optimized in proportion to the relative surface area and the number of cells ...
-
bioRxiv - Cell Biology 2021Quote: ... 4.5 μl X-tremeGENE 9 in 100 μl serum-free OptiMEM) or PEI transfection reagent (1 mg ml−1 stock, Polyscience 24765-2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were mounted on microscope slides in Aqua polymount (Polyscience). Primary antibodies used ...
-
bioRxiv - Biophysics 2023Quote: ... Probe particles (Polybead® Microspheres 1 μm, Polyscience, Inc) were suspended in 60% Glycerol (ROTIPURAN® ...
-
bioRxiv - Molecular Biology 2021Quote: Cell transfections were performed using polyethyleneimine (PEI) reagent (Polyscience Inc., 23966) or jetPRIME (Polyplus transfection ...
-
bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 1.2 µL of 1 mg/mL PEI MAX (Polyscience #24765), and 100 µL of 1x PBS were mixed ...
-
bioRxiv - Neuroscience 2024Quote: ... counterstained with DAPI (1:1000) and mounted with AquaPolymount (Polyscience). High-resolution images dendritic spines were acquired using a Nikon A1 Eclipse confocal microscope equipped with NIS element AR v4.20.03 software (Nikon ...
-
bioRxiv - Immunology 2022Quote: ... The recombinant vectors were transiently transfected into HEK293F cells with polyethyleneimine (Polyscience). After three days of expression ...
-
bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were co-transfected using PEI-MAX (1μg/ml; Polyscience, 324765) with VSV-G (Addgene#8454) ...
-
bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
-
bioRxiv - Microbiology 2024Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
-
bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...
-
bioRxiv - Neuroscience 2022Quote: ... and an ITR-flanked transgene using polyethylenimine (PEI, Polyscience, 24765-1). AAVs were harvested from the cells and the media 3 days post transfection and purified by ultracentrifugation over iodixanol gradients ...
-
bioRxiv - Cell Biology 2023Quote: ... 300 µL of 1 mg/mL polyethylenimine linear 25k (Polyscience, 23966) was diluted into 5 mL OptiMEM in a separate tube ...
-
bioRxiv - Neuroscience 2023Quote: ... blue fluorescent neuronal tracer fast blue (Polyscience Inc, 17740-1, 1mg) onto the surface of L1 for 5 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... we used the PEI transfection reagent (Linear PEI25K, PolyScience; 23966-1). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... The expression vectors were transiently transfected to human HEK293F cells with polyethylenimine (Polyscience). Three days later ...
-
bioRxiv - Biochemistry 2023Quote: ... cells suspended in washing buffer were Concanavalin A coated magnetic beads (Polyscience, 86057) for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... and 10 µg lentiviral plasmid using PEI-Max (Polyscience, cat.no. 24765-1). Lentiviral supernatant was harvested 36 ...
-
bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected using the PEImax (Cat# 24765-2, MW 40,000, Polyscience, Inc.) at a ratio of 1:3 of DNA ...
-
bioRxiv - Bioengineering 2023Quote: ... One (1) μm yellow PSP and 6 μm red PSP were from Polyscience Inc ...