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Citations for Lucigen :
101 - 150 of 212 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The resulting cDNA was circularized by 40 U ssDNA ligase (Lucigen) at 60 °C for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified cDNA was then circularized using CircLigaseII ssDNA ligase (Epicentre) for 1[h at 60°C and was subsequently PCR amplified using the primers 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC-3′ and 5′-CAAGCAGAAGACGGCATACGAGATJJJJJJGTGACTGGAGTTCAGACGTGTG-3′(where Js indicates the 6-mer index sequence for Illumina sequencing).
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bioRxiv - Biochemistry 2023Quote: ... cDNA was column-purified and circularized with CircLigase (Epicentre/Illumina, CL9021K) for 16hrs ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted cDNA was circularized with CircLigase II ssDNA ligase (Lucigen, CL9021K) for 1 hr at 60°C ...
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bioRxiv - Microbiology 2022Quote: ... two mid-vaginal specimens were self-collected by the subject using sterile Catch-All™ Sample Collection Swabs (Epicentre Biotechnologies) and placed at −20°C ...
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bioRxiv - Molecular Biology 2022Quote: Approximately 1e+06 cells were harvested two days post nucleofection and incubated in 200 μL of QuickExtract DNA Extraction Solution (Lucigen) at 65ºC for 15 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μl per tube was transformed into two tubes of 50 μl of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: A Texas fosmid library was constructed in the pNGS vector (Lucigen). Sixty-eight pools of ∼500 clones per pool were made ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared with the ScriptSeq v2 kit (Epicentre) and subjected to paired-end sequencing (2 x 100 bp ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA libraries were then prepared using ScriptSeq v2 Stranded Kit (Epicentre) following the manufacturer’s protocol except fragmentation was done at 85°C for 8min ...
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bioRxiv - Molecular Biology 2019Quote: ... The strand-specificity of Scriptseq libraries is >98% (Epicentre product literature) therefore contigs with < 98% of forward strand reads were considered being derived from dsRNA rather than possible contaminating ssRNA.
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the ScriptSeq v2 kit (EpiCentre). Total RNA was depleted using RiboZero Gold (EpiCentre ...
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bioRxiv - Genomics 2020Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, Cat: CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was circularized by CircLigase™ II ssDNA Ligase (Epicentre, CL9025K). Next ...
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bioRxiv - Microbiology 2022Quote: ... Gel-purified cDNAs were then circularized with CircLigase II (Lucigen, Cat: CL4115K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Microbiology 2023Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, cat. CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting cDNA products were circularized with CircLigase ssDNA ligase (Lucigen, USA). Illumina sequencing adaptors were introduced by PCR and the final library sequenced at the JHU Genetic Resources Core Facility on a HiSeq2500 (Illumina ...
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bioRxiv - Synthetic Biology 2022Quote: ... two ncRNA expression cassettes (for barcoded ncRNAs “A” and “B”) from the Marionette plasmids were cloned into pSol-TSF (Lucigen F843213-1) facing in opposite directions ...
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bioRxiv - Microbiology 2020Quote: ... were collected by swabbing the inner pinna of the ear of two dogs using Sterile Catch-All™ Sample Collection Swabs (Epicentre Biotechnologies). DNA was extracted with QIAGEN-DNeasy PowerSoil Kit ...
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bioRxiv - Immunology 2021Quote: ... bisulfite-modified DNA sequencing libraries were generated using the EpiGenome kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the CopyControl Fosmid Library Production Kit with pCC1FOS vectors (Epicentre, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... RNA-Seq libraries were prepared using Scriptseq Complete Gold Yeast Kit (Epicentre). Ribo-Seq libraries were prepared essentially as per Ingolia and colleagues (18 ...
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bioRxiv - Immunology 2020Quote: ... bisulfite-modified DNA sequencing libraries were generated using the EpiGenome kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... rRNA depletion for the mRNA library was conducted with Ribozero kit (Epicentre) with the low input protocol ...
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bioRxiv - Microbiology 2022Quote: ... The library was electroporated into Endura electrocompetent cells (# 60242; Lucigen Bioresearch Technologies) and plasmid was prepared from > 80 million bacterial colonies (>1,000X representation) ...
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bioRxiv - Cell Biology 2023Quote: ... Library (50ng) was electroporated into 25µL Endura electrocompetent cells (60242-2, Lucigen). Cells from eight electroporations were pooled and rescued in 8mL of rescue media for 1hr at 37 ...
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bioRxiv - Biochemistry 2019Quote: ... the dissolved cDNA was mixed with 3 μl of 10x CircLigase Buffer (Epicentre), 1.5 μl of 50 mM MnCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was generated with EpiScript RT enzyme (Lucigen, 50C for 30 minutes) followed by reaction clean up with exonuclease I (Lucigen ...
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bioRxiv - Genomics 2022Quote: ... The final plasmid library was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Bioengineering 2020Quote: ... The cloned libraries were individually amplified by electroporation into Endura ElectroCompetent cells (Lucigen). Using dilution plates for colony counting ...
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bioRxiv - Genomics 2021Quote: ... 2 uL of library ligation was added to 50 uL Endura cells (Lucigen) then electroporated ...
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bioRxiv - Microbiology 2021Quote: ... ScriptSeq™ v2 RNA-Seq library preparation kit (Epicentre, Illumina, Madison, WI USA) was used for this purpose ...
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bioRxiv - Genomics 2021Quote: ... The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen) and 100 ng plasmid libraries per electroporation reaction as previously described24 ...
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bioRxiv - Microbiology 2023Quote: ... and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina) (76) ...
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bioRxiv - Biochemistry 2023Quote: The NDM libraries are then transformed into Escherichia coli (E. cloni 10G, Lucigen) and stored as glycerol stocks ...
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bioRxiv - Genomics 2024Quote: ... The purified library was electroporated in eight replicates into Endura electrocompetent cells (Lucigen), using 50-100 ng/μl DNA and 25 μl cells in 0.1 cm BioRad cuvettes at 1800 V ...
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bioRxiv - Genomics 2020Quote: ... First strand cDNA synthesis was done using Episcript™ RNase H-reverse transcriptase (Epicentre) where RNA samples were primed with random hexamers (NEB) ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... The size-selected and purified cDNA was circularized using CircLigase ssDNA ligase kit (Lucigen) following manufacture’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... the fragmentation of 100 pg cDNA was performed with EZ Tn5 Transposase (Lucigen, TNP92110). Fragments of cDNA were amplified by KAPA HiFi hotstart readymix (EMSCO/FISHER ...
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bioRxiv - Cell Biology 2020Quote: ... for depletion of rRNA followed by library preparation using the ScriptSeq v2 technology (Epicentre). The RNA-Seq libraries were combined into 2 nM pooled stocks ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of library was added to 25 µL of Endura electrocompetent cells (Lucigen). Cells were electroporated using the MicroPulser (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... The custom gRNA library was amplified in electrocompetent Endura bacteria (Lucigen, Cat. No. 60242) and plasmid DNA purified as described ...
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bioRxiv - Cell Biology 2019Quote: ... expanded by 16 electroporations (8 for each half library) into Endura electrocompetent cells (Lucigen), and plated on sixteen 24.5 cm bioassay plates (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... The bisulfite-modified DNA-sequencing library was generated using the EpiGnome™ kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cosmid libraries were constructed using MaxPlax™ Lambda Packaging Extracts (Lucigen Corp, Middleton, WI).
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Biochemistry 2023Quote: ... the library was electroporated into Escherichia coli cells (E. cloni 10G Elite; Lucigen, USA), yielding ≈ 107 colonies after overnight incubation on agar plates ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...