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Citations for Lucigen :
1 - 50 of 212 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA libraries were circularized by two sequential CircLigase (Epicentre) reactions and amplified by 9-12 PCR cycles.
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bioRxiv - Genomics 2023Quote: ... Two 40 Kb mate-pair libraries were generated by Lucigen from 1 µg of gDNA using the CviQl and BfaI restriction enzymes ...
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bioRxiv - Immunology 2021Quote: ... libraries were constructed from first strand cDNA using ScriptSeqTM v2 RNA-Seq library preparation kit (Epicentre Biotechnologies, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries were prepared using the ScriptSeqTM Complete Kit (Epicentre, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cDNA libraries were generated using a strand-specific ScriptSeq Complete Kit Bacteria protocol (Epicentre), indexed and sequenced on an Illumina HiSeq 2000 platform at the Lausanne Genomics Facilities with 101-nt single-end reads ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the ScriptSeq™ Complete Kit (Epicentre, Madison, WI, USA) according to the manu-facturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis and strand-specific library generation was carried out using the Script-Seq V2 Kit (Epicentre) and the FailSafe PCR enzyme mix ...
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bioRxiv - Genetics 2021Quote: ... The DNA:RNA hybrids were digested with 10 U Thermostable RNase H (Epicentre, H39500) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA-DNA hybrids were specifically digested with Hybridase Thermostable RNase H (Epicentre) at 45 °C for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... The gRNA-msDNA hybrid was extracted with the QuickExtract RNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Metatranscriptomic libraries were prepared for sequencing with the addition of 5–50 ng of RNA to the ScriptSeq cDNA V2 library preparation kit (Epicentre). Metatranscriptomic samples were sequenced with an Illumina NextSeq 500 system using V2 high output 300 cycle reagent kit with PHIX control added ...
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bioRxiv - Genetics 2021Quote: ... Total genomic DNA of hybrids was extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen). DNA concentration was measured using the Qubit dsDNA HS assay (ThermoFischer ...
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bioRxiv - Microbiology 2021Quote: ... Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc., Madison, WI, USA) in accordance with the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2020Quote: ... Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeq™ v2 RNA-Seq Library Preparation guide (EpiCentre Inc., Madison, WI, USA) in accordance with the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2024Quote: ... Between 200- 500 ng of enriched mRNA was used for cDNA production using the ScriptSeq®v2RNA-Seq Library Preparation Kit (Epicentre, Madison, WI, USA), FailSafe®PCR Enzyme Mix (Epicentre ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... waltii isolates as well as the generated hybrids were extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen).
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bioRxiv - Genetics 2021Quote: ... and libraries prepared using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre), PCR amplified in 9 cycles ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared with ScriptSeq v2 RNA-seq library preparation kit (Epicentre) and sequencing was performed on NextSeq500 (single-end 96 bp ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NxSeq AmpFREE Low DNA Fragment Library Kits (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and libraries were constructed using the NxSeq AmpFREE low DNA Library Kit by Lucigen. Bisulfite conversion was done using the EZ-96 DNA Methylation-GoldTM MagPrep kit (D5042 ...
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bioRxiv - Genomics 2019Quote: ... We constructed a fosmid library using the Copy Control Fosmid Library Production Kit (Epicentre) and screened it using DIG-labeled (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing libraries were generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) using 12 PCR cycles for amplification ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA library was prepared using a NxSeq AmpFREE Low DNA Library kit (Lucigen) and sequenced on a Illumina HiSeq2000 with a 100bp paired-end mode ...
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bioRxiv - Microbiology 2020Quote: ... PCR-free library preparation (Lucigen) and NextSeq 500 sequencing (Illumina ...
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL; Lucigen glass filter and PALL) were also sequenced to reveal contaminants ...
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bioRxiv - Genomics 2022Quote: Shot-gun library was generated at the McGill Genome Center using the NxSeq® AmpFREE Low DNA Library Kit Library Preparation Kit (Lucigen Corp., WI, USA) according to the manufacturer’s recommendations and the library was ran on a HiSeq X for 2×150 cycles.
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was circularized with CircLigase (Epicentre), pooled and then amplified with PCR primers with Illumina sequencing adapters as desribed previously 55,56 ...
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the Quantseq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Directional libraries were prepared with the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) and single end sequencing was performed on a Hi-Seq 2000 platform at the Deep Sequencing Core Facility at the University of Massachusetts Medical School in Worcester ...
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bioRxiv - Molecular Biology 2021Quote: ... Two micrograms of starting material were treated with Baseline-ZERO™ DNase (Lucigen, USA) and ribosomal RNA was removed using the Ribo-Zero™ Magnetic Core Kit and Ribo-Zero™ rRNA Removal kit (Illumina ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Systems Biology 2020Quote: ... RNA-seq libraries were constructed using ScriptSeq™ v2 RNA-Seq Library Preparation kits (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA sequencing library was generatd using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: Directional multiplex libraries were prepared with the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) and paired end sequencing was performed on a Hi-Seq 2000 platform at the Deep Sequencing Core Facility at the University of Massachusetts Medical School in Worchester ...
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bioRxiv - Genomics 2021Quote: ... The libraries were generated using the NxSeq AmpFREE Low DNA Library Kit and NxSeq Adaptors (Lucigen). They were then sequenced on an Illumina HiSeq X (PE150).
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bioRxiv - Molecular Biology 2021Quote: ... The cDNAs were circularized by CircLigaseII (Lucigen) and PCR-amplified ...
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bioRxiv - Genomics 2020Quote: ... cDNA was circularized with CircLigase (Epicentre, CL4111K). DNA libraries were amplified by PCR for 6-10 cycles with Phusion HF DNA Polymearse (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was circularized by Circligase (Lucigen) and then re-linearized by BamHI ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA samples were used for library construction using ScriptMiner Small RNA-seq library preparation Kit (Epicentre) and sequencing was performed on an Illumina NextSeq500.
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (Catalogue Number: 14000-1, Lucigen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... library preparation was performed using ScriptSeq (EpiCentre) followed by sequencing on Illumina HiSeq 2000 or KAPA RNA HyperPrep Kit (RiboErase HMR ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of T5-Tn5 transposome and 4 μl of T7-Tn5 transposome were mixed to form 8 μl of assembled Tn5 transposome before it was mixed with 1 μl cDNA (containing ~500 pg cDNA) and 1 μl reaction buffer (TNP92110, Lucigen). The reaction mixture was incubated at 37°C for 1 h ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... cDNA was then circularized with CircLigase II (Epicentre) and used for library PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... This cDNA was then circularized using CircLigase (Lucigen) and barcodes and full Illumina adapters were added through PCR using Phusion (NEB).
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was then circularized with CircLigase II (Epicentre) and used for library PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was circularized using 0.5 uL CircLigase (Epicentre), 1 uL CircLigase buffer ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was circularized with CircLigase II (CL9021, Lucigen). Libraries were PCR amplified using Phusion DNA Polymerase (M0530 ...
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bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...