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Citations for Lucigen :
251 - 300 of 466 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the cells were re-suspended in 20 μl of DNA Extraction Solution (Lucigen), and incubated at 68°C for 6 min ...
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bioRxiv - Molecular Biology 2020Quote: Harvested cells were lysed using QuickExtract DNA Extraction Solution (Lucigen, Cat. No. QE09050) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The genomic DNA of cells was extracted using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: ... The final plasmid library was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Genetics 2019Quote: ... The cell pellet was agitated in 100 μL of QuickExtract DNA Solution (Lucigen) to disrupt the pellet and placed in a thermocycler for 15 minutes at 68°C followed by 10 minutes at 95°C ...
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bioRxiv - Bioengineering 2020Quote: ... The cloned libraries were individually amplified by electroporation into Endura ElectroCompetent cells (Lucigen). Using dilution plates for colony counting ...
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bioRxiv - Genomics 2021Quote: ... 2 uL of library ligation was added to 50 uL Endura cells (Lucigen) then electroporated ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA from each cell line was isolated (QuickExtract DNA Extraction Solution, Lucigen). Total RNA was isolated from each cell line (Maxwell RSC simplyRNA kit ...
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bioRxiv - Molecular Biology 2021Quote: ... Competent cells were electroporated with Ez-Tn5
transposome (Lucigen, Madison, WI). Cells recovered for 90 minutes at 30 °C and then played on PYE plates supplemented with kanamycin ... -
bioRxiv - Genomics 2021Quote: ... The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen) and 100 ng plasmid libraries per electroporation reaction as previously described24 ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from the cells using QuickExtract DNA Extract Solution (Lucigen, QE9050). Clones were genotyped by PCR and Sanger Sequencing (Supplementary Fig ...
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bioRxiv - Microbiology 2020Quote: ... The cells were resuspended and lysed by 120 kU Ready-Lyse lysozyme (Lucigen), 400 U RNase I (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... pET15b/MBP-Vpu plasmid was transfected into E.coli BL21(DE3) competent cells (Lucigen) following the recommended by the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage selections were amplified by adding them to 1.0 ml TG1 cells (Lucigen) grown to mid-log phase (OD ∼ 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 500 μL of Red blood cell lysis buffer (Lucigen, SS000400-D2) and incubated at room temperature for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from cell pellets with QuickExtract DNA Extraction Solution (Lucigen) via manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: DNA from GFP-positive cells was isolated using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were collected and gDNA was isolated with Quick Extract (Lucigen) following manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... The purified library was electroporated in eight replicates into Endura electrocompetent cells (Lucigen), using 50-100 ng/μl DNA and 25 μl cells in 0.1 cm BioRad cuvettes at 1800 V ...
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bioRxiv - Developmental Biology 2021Quote: The cell pellets of picked colonies were resuspended in 10 µl QuickExtract (Epicentre, QE0905T) and the suspension was incubated at 65 °C for 10 min ...
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bioRxiv - Genomics 2020Quote: The genomic DNA of collected cells was extracted using QuickExtract DNA Extraction Solution (Epicentre). Genomic PCR reaction was performed using 100 ng genomic DNA ...
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bioRxiv - Bioengineering 2020Quote: ... The products of the Gibson reactions were combined and electroporated into Endura cells (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed and genomic DNA was extracted using QuickExtract DNA Extraction Buffer (Lucigen) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and RNA extracted with MasterPure™ Yeast RNA Purification Kit (Epicentre). Samples were vacuum dried and sent to Genewiz (South Plainfield ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cell lines using QuickExtract DNA Extraction Solution (Epicentre, #QE09050). Specifically ...
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bioRxiv - Genetics 2022Quote: ... The ligated product was then electroporated into Enduratm electro-competent cells (Lucigen #60242-1) in 2 separate reactions ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of library was added to 25 µL of Endura electrocompetent cells (Lucigen). Cells were electroporated using the MicroPulser (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transformed into E.cloni ® 10G Chemically Competent Cells (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... genomic DNA was isolated from the transfected cells using DNA QuickExtract™ Buffer (Lucigen) in adherence to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using the MasterPure RNA Purification Kit from Epicentre following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µl from ligation reaction was added onto 25 µl of electrocompetent cells (Lucigen) and cells were transformed by using Electroporator (Bio-Rad MicroPulser ...
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bioRxiv - Cell Biology 2019Quote: ... expanded by 16 electroporations (8 for each half library) into Endura electrocompetent cells (Lucigen), and plated on sixteen 24.5 cm bioassay plates (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage of around 200x.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... the library was electroporated into Escherichia coli cells (E. cloni 10G Elite; Lucigen, USA), yielding ≈ 107 colonies after overnight incubation on agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed and solubilised with 2000 U of Ready-Lyse Lysozyme (Epicentre) and 0.5% SDS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA of 3T3 cells was isolated with QuickExtractTM DNA Extraction Solution 1.0 (QE09050, Lucigen). Presence of the homozygous deletion was confirmed by PCR using specific primers ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from transfected cells using the QuickExtractTM DNA Extraction Solution (Lucigen) and mutations were detected by the SURVEYOR assay (Integrated DNA technologies) ...
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bioRxiv - Genomics 2023Quote: ... 1.5 µL BP reaction was electroporated into 25 µL Endura Electrocompetent cells (Lucigen, 60242) with the following conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Genomics 2019Quote: ... One microliter of the reaction mix was used to transform ultra-competent EPI300 cells (Epicentre). Telomere-positive clones were identified by colony blotting.
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bioRxiv - Immunology 2019Quote: ... Genomic DNA was extracted from candidate RAB1B KO cells using QuickExtract DNA Extraction Solution (Lucigen), as per manufacturer’s instructions ...