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Citations for Lucigen :
151 - 200 of 466 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen 60052-4) in 1mm cuvette (Bio-Rad 1652089 ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were lysed by QuickExtraction solution (Lucigen) per the manufacturer’s instruction at 65°C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen). All cloning procedures were verified by Sanger sequencing using BigDye® Terminator v3.1 Cycle Sequencing kit on 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested using Quick Extract solution (Lucigen). Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548 ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... TOPO plasmids were electroporated into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... The purified product was transformed into electrocompetent cells (Lucigen) via a MicroPulser (Bio-Rad) ...
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bioRxiv - Biophysics 2020Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen Corporation, Middleton, USA) were transformed with a pT7 plasmid containing the ClyA-AS gene ...
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bioRxiv - Immunology 2021Quote: ... Recombinant phagmids were electroporated into TG1 cells (Lucigen, USA). A VHH library of 7.3 × 106 individual clones was obtained.
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bioRxiv - Biophysics 2020Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) followed by 0.5% SDS treatment ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were thoroughly resuspended in 50 µl QuickExtract (Lucigen) and incubated at 65 °C for 20 min and 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligated product was electroporated into Electrocompetent cells (Lucigen) and grown in liquid culture overnight at 37 degrees before being extracted by Maxiprep ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) in TE for 60 min ...
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bioRxiv - Genetics 2023Quote: ... The cell pellet was resuspended in Quick-Extract (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... TG1 electrocompetent cells were procured from Lucigen (Middleton, WI). M13KO7 helper phage particle ...
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bioRxiv - Genomics 2023Quote: ... then were transformed into Endura Electrocompetent Cells (Lucigen, 602422) by electroporation (Gene Pulser Xcell Electroporation System ...
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bioRxiv - Cancer Biology 2023Quote: ... The final product was transformed into electrocompetent cells (Lucigen), incubated overnight in LB broth at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... cloni 10G SUPREME electrocompetent cells (Lucigen Cat#60080-2) using a MicroPulser Electroporator (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The electroporated cells were recovered in recovery medium (Lucigen) for 1 h and then plated on Terrific Broth (TB ...
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Genomics 2020Quote: ... electroporated in triplicate into Endura electrocompetent cells (Lucigen, Middleton WI), and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Microbiology 2021Quote: ... The vector was transformed into BigEasy-TSA Electrocompetent Cells (Lucigen) for propagation ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was electroporated into Endura competent cells (Lucigen) using 50-100ng DNA per electroporation (we set up 6 or 24 replicate transformation for tiling or genome-wide sgRNA library to maintain library complexity) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed using QuickExtract DNA Extraction Solution (Lucigen) following supplier’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli 10G electrocompetent cells (60080-2, Lucigen, Middleton, WI, US). Cells were selected with appropriate antibiotics on solid and liquid culture ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet resuspended in 100μL QuickExtract solution (Lucigen #QE09050), transferred to PCR-tubes and incubated in a thermocycler for 15 minutes at 68°C ...
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bioRxiv - Bioengineering 2021Quote: ... Each library was independently electroporated into Endura electrocompetent cells (Lucigen), plated on agar bioassay plates ...
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bioRxiv - Genetics 2019Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was extracted from HEP3B cells using QuickExtract (Epicentre).
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bioRxiv - Genomics 2022Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells using the MasterPure Yeast RNA Purification Kit (Epicentre). The procedure for removal of DNA contamination was as per the specification of the manufacturer but performed twice to efficiently remove the DNA from the samples ...
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bioRxiv - Plant Biology 2022Quote: ... the following plasmids were transformed into BL21DE3-C41 cells (Lucigen): codon optimized pET- DUET1:ZmCPR2/ZmCYP71Z18 or ZmCYP71Z16 ...
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bioRxiv - Genomics 2022Quote: ... Coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Molecular Biology 2023Quote: ... and cells were lysed using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Cell Biology 2023Quote: ... followed by electroporation into Endura ElectroCompetent Cells (Lucigen, 60242-2) at ∼100x coverage ...
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bioRxiv - Systems Biology 2022Quote: ... gDNA was isolated from obtained cell clones using QuickExtract (Lucigen), the gRNA-targeted sites were PCR-amplified ...
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bioRxiv - Genetics 2023Quote: ... 5 Gibson reactions were electroporated into supercompetent cells (Endura, Lucigen) and grown in liquid culture to give a coverage of around 100 million barcodes ...
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bioRxiv - Biochemistry 2023Quote: ... coli (C41) cells for protein expression were purchased from Lucigen Corporation (Wisconsin) ...
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bioRxiv - Genetics 2023Quote: ... The purified product was electroporated into Endura competent cells (Lucigen). We extracted the plasmid pool using the Plasmid Maxi prep kit ...
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bioRxiv - Bioengineering 2023Quote: ... cells were lysed by QuickExtract™ DNA Extraction Solution (Lucigen) to extract genomic DNA ...
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bioRxiv - Genetics 2023Quote: ... then electroporated 10 uL into Endura competent cells (Lucigen #602422) and then expanded the cells in liquid culture for 18 hours at 30°C and purified library plasmid with the Nucleobond Xtra Midi EF kit (Machery-Nagel #740420.50) ...
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bioRxiv - Neuroscience 2023Quote: ... We also propagated SCN1A into CopyCutter EPI400 cells (formerly Lucigen, now BioSearch Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... and expressed in BL-21 (DE3) cells (Lucigen, Middleton, WI). Bacterial cultures were grown overnight at 37°C in 2x YT (Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then thawed and 35,000 U of Ready-Lyse (Epicentre) was added ...
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bioRxiv - Genomics 2020Quote: ... They were then amplified using electroporation in Endura electrocompetent cells (Lucigen), to achieve at least 250 colonies per sgRNA in the library ...
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bioRxiv - Genomics 2020Quote: ... using a Gibson assembly master mix (New England Biolabs).Gibson assembly products were transformed into electrocompetent cells (E. cloni, Lucigen) and plated on 245mm x 245mm square LB-agar plates to obtain the sufficient number of bacterial colonies at a ∼50× library coverage ...
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bioRxiv - Bioengineering 2019Quote: ... genomic DNA was extracted from cells using Quick Extract (Lucigen, QE09050) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... coli Cloni® 10G cells (Lucigen, USA; 1ul crude reaction mix). Kanamycin selected clones were screened by colony PCR and verified by sequencing ...