Labshake search
Citations for Lucigen :
301 - 350 of 470 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA of 3T3 cells was isolated with QuickExtractTM DNA Extraction Solution 1.0 (QE09050, Lucigen). Presence of the homozygous deletion was confirmed by PCR using specific primers ...
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bioRxiv - Genomics 2023Quote: ... 1.5 µL BP reaction was electroporated into 25 µL Endura Electrocompetent cells (Lucigen, 60242) with the following conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Genomics 2019Quote: ... One microliter of the reaction mix was used to transform ultra-competent EPI300 cells (Epicentre). Telomere-positive clones were identified by colony blotting.
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bioRxiv - Immunology 2019Quote: ... Genomic DNA was extracted from candidate RAB1B KO cells using QuickExtract DNA Extraction Solution (Lucigen), as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from pelleted cells using a MasterPure Yeast RNA extraction kit (Lucigen, MPY03100), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The genomic DNA from individual cell clones was extracted using QuickExtract DNA Extraction Solution (Lucigen) and screened by PCR for the deletion amplicon using the DNA primers listed in Supplementary Table 1 ...
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bioRxiv - Genomics 2022Quote: ... the clean-up DNAs underwent the protocol SYGNIS TruePrimeTM WGA & Single Cell WGA Kits (Lucigen), instead of the REPLI-g single cell kit ...
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bioRxiv - Developmental Biology 2022Quote: ... Single cells were FACS sorted into 1 ul each of QuickExtract DNA Extraction Buffer (Lucigen). DNA was extracted by incubating cells for 10 minutes at 65°C followed by 5 minutes at 98°C ...
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bioRxiv - Genomics 2020Quote: ... ethanol precipitated and transformed into 200l of electrocompetent E.coli (Lucigen Endura™ ElectroCompetent Cells, Lucigen). 4 x 250ml LB preps (supplemented with 100 μg mL-1 carbenicillin ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from at least 104 harvested cells using the QuickExtract protocol (Lucigen). The target locus was then amplified by PCR with forward and reverse primers where at least one primer annealed outside the integration site (Supplementary Table 1) ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 104 to 105 T cells was was extracted using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using primers F2(RB198)* and R2(RB199)* primers (Supplemental Table 1) ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and positional insertion of mGFP/mScarlet-I cassette ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). Primers specific to the blasticidin resistance cassette and IRAK1/IRAK4 gene loci were used to verify the insertion (see Table S5 and S6 for IRAK4 and IRAK1 respectively) ...
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Intra-species transcriptional profiling reveals key regulators of Candida albicans pathogenic traitsbioRxiv - Genomics 2021Quote: ... RNA was harvested from cells using a Masterpure Yeast RNA Purification kit (Epicentre, Madison, WI) and treated with DNaseI (Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from each of the four cell pools with 100 µL QuickExtract (Lucigen) per 1 million cells following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from cell pellets using the MasterPure yeast DNA purification kit (Epicentre). Melanin was removed from genomic DNA using the OneStepTM PCR Inhibitor Removal kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Genomics 2023Quote: ... Resulting colonies were picked and a portion of the cells lysed by QuickExtract (Lucigen, QE9050) and used to genotype by PCR with primers on either side of the insertion site (in most cases with one primer outside the homology arms ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and correct insertion of mGFP/mScarlet-i cassette ...
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bioRxiv - Molecular Biology 2023Quote: ATAC-see in 293T cells was done with the EZ-Tn5™ Transposase (Lucigen TNP9211), following the published protocol36 ...
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bioRxiv - Bioengineering 2023Quote: ... each reaction mixtures were transformed into Endura™ electro-competent cells (Lucigen, CAT. 60242-1) and transformed bacteria were selected on LB + Ampicillin plates ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were then introduced into BAC-Optimized Replicator v2.0 electrocompetent cells (60210, Lucigen) via electroporation at 2500V ...
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bioRxiv - Microbiology 2024Quote: ... pneumoniae ECL8 electrocompetent cells were transformed with 0.2 µL of EZ-Tn5™ transposon (Epicentre) at 1.4 kV and recovered in brain heart infusion (BHI ...
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bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 2 µl water and subjected to electroporation into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: Genomic DNA was isolated from candidate cell lines using the QuickExtract DNA Extraction Solution (Lucigen, #QE09050) essentially as described ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from bacterial cell pellets using MasterPure™ complete DNA purification kit (Lucigen). Oxford Nanopore Minion Sequencing ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA from 2.5E5 cells was extracted with 250 µl of QuickExtract DNA extraction solution (Lucigen) per manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from sorted and non-sorted cells using the QuickExtract reagent (Lucigen QE09050). In addition ...
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bioRxiv - Systems Biology 2020Quote: RFP positive A549 VIM-RFP cells were harvested and DNA was extracted using QuickExtract (Epicentre, QE09050). Primers were designed for left homology arm and right homology arm junction PCR (left junction Fwd ...
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bioRxiv - Systems Biology 2021Quote: ... We performed nine electroporations of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: For single cell clones or bulk sequencing genomic DNA (gDNA) was extracted by quick extract (Lucigen). PCR amplification was performed with LongAmp Polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... The CCF stool pellets were homogenized with 500 μL Tissue and cell lysis buffer (Lucigen©) by pipetting up and down ...
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bioRxiv - Immunology 2021Quote: ... 40 nanograms of each library were transformed into Endura™ ElectroCompetent Cells (Lucigen cat 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting ligation mixture was electroporated into TG-1 phage-display competent cells (#60502-1, Lucigen) and plated onto 2xYT agar containing 100 µg/mL carbenicillin and 2% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets were collected 72-96 hours after electroporation and resuspended in QuickExtract solution (Lucigen, QE09050) and subjected to genomic DNA extraction while incubating for 10 min at 65 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... the pBA439_UMI20 library was electroporated into 25 μl of Lucigen Endura ElectroCompetent Cells (Lucigen, 60242-2) using prechilled 1 mm electroporation cuvettes (BioRad ...
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bioRxiv - Genomics 2023Quote: Single-cell DNA extractions were performed using the MasterPure DNA and RNA Purification Kit (Epicentre Biotechnologies) following the protocol as written ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Three hundred nanograms of purified plasmids were electroporated into 25 µl of Endura electrocompetent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The pDNA for IVT were isolated from the E.coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen) using the Plasmid Maxi Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA templates were prepared by lysing cells in QuickExtract DNA extraction solution (Lucigen, Cat No. QE0905T). Target regions were amplified by using specific PCR primers (ETV2_F ...