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Citations for Lucigen :
251 - 300 of 470 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... cells were expanded and genomic DNA extracted (QuickExtract DNA Extraction Solution; Epicentre, #QE09050), as described above ...
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bioRxiv - Biophysics 2022Quote: ... DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.
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bioRxiv - Bioengineering 2022Quote: DNA was extracted from harvested cells using the QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... Genome DNA was extracted from survival cells using QuickExtract DNA Solution 1.0 (Epicentre), and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were re-suspended in 20 μl of DNA Extraction Solution (Lucigen), and incubated at 68°C for 6 min ...
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bioRxiv - Molecular Biology 2020Quote: Harvested cells were lysed using QuickExtract DNA Extraction Solution (Lucigen, Cat. No. QE09050) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The genomic DNA of cells was extracted using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: ... The final plasmid library was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Genetics 2019Quote: ... The cell pellet was agitated in 100 μL of QuickExtract DNA Solution (Lucigen) to disrupt the pellet and placed in a thermocycler for 15 minutes at 68°C followed by 10 minutes at 95°C ...
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bioRxiv - Bioengineering 2020Quote: ... The cloned libraries were individually amplified by electroporation into Endura ElectroCompetent cells (Lucigen). Using dilution plates for colony counting ...
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bioRxiv - Genomics 2021Quote: ... 2 uL of library ligation was added to 50 uL Endura cells (Lucigen) then electroporated ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA from each cell line was isolated (QuickExtract DNA Extraction Solution, Lucigen). Total RNA was isolated from each cell line (Maxwell RSC simplyRNA kit ...
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bioRxiv - Molecular Biology 2021Quote: ... Competent cells were electroporated with Ez-Tn5
transposome (Lucigen, Madison, WI). Cells recovered for 90 minutes at 30 °C and then played on PYE plates supplemented with kanamycin ... -
bioRxiv - Genomics 2021Quote: ... The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen) and 100 ng plasmid libraries per electroporation reaction as previously described24 ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from the cells using QuickExtract DNA Extract Solution (Lucigen, QE9050). Clones were genotyped by PCR and Sanger Sequencing (Supplementary Fig ...
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bioRxiv - Microbiology 2020Quote: ... The cells were resuspended and lysed by 120 kU Ready-Lyse lysozyme (Lucigen), 400 U RNase I (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... pET15b/MBP-Vpu plasmid was transfected into E.coli BL21(DE3) competent cells (Lucigen) following the recommended by the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage selections were amplified by adding them to 1.0 ml TG1 cells (Lucigen) grown to mid-log phase (OD ∼ 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 500 μL of Red blood cell lysis buffer (Lucigen, SS000400-D2) and incubated at room temperature for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Molecular Biology 2023Quote: DNA from GFP-positive cells was isolated using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from cell pellets with QuickExtract DNA Extraction Solution (Lucigen) via manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were collected and gDNA was isolated with Quick Extract (Lucigen) following manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... The purified library was electroporated in eight replicates into Endura electrocompetent cells (Lucigen), using 50-100 ng/μl DNA and 25 μl cells in 0.1 cm BioRad cuvettes at 1800 V ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Developmental Biology 2021Quote: The cell pellets of picked colonies were resuspended in 10 µl QuickExtract (Epicentre, QE0905T) and the suspension was incubated at 65 °C for 10 min ...
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bioRxiv - Genomics 2020Quote: The genomic DNA of collected cells was extracted using QuickExtract DNA Extraction Solution (Epicentre). Genomic PCR reaction was performed using 100 ng genomic DNA ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The products of the Gibson reactions were combined and electroporated into Endura cells (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed and genomic DNA was extracted using QuickExtract DNA Extraction Buffer (Lucigen) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and RNA extracted with MasterPure™ Yeast RNA Purification Kit (Epicentre). Samples were vacuum dried and sent to Genewiz (South Plainfield ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cell lines using QuickExtract DNA Extraction Solution (Epicentre, #QE09050). Specifically ...
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bioRxiv - Genetics 2022Quote: ... The ligated product was then electroporated into Enduratm electro-competent cells (Lucigen #60242-1) in 2 separate reactions ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of library was added to 25 µL of Endura electrocompetent cells (Lucigen). Cells were electroporated using the MicroPulser (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transformed into E.cloni ® 10G Chemically Competent Cells (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... genomic DNA was isolated from the transfected cells using DNA QuickExtract™ Buffer (Lucigen) in adherence to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using the MasterPure RNA Purification Kit from Epicentre following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µl from ligation reaction was added onto 25 µl of electrocompetent cells (Lucigen) and cells were transformed by using Electroporator (Bio-Rad MicroPulser ...
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bioRxiv - Cell Biology 2019Quote: ... expanded by 16 electroporations (8 for each half library) into Endura electrocompetent cells (Lucigen), and plated on sixteen 24.5 cm bioassay plates (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage of around 200x.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage ...
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bioRxiv - Biochemistry 2023Quote: ... the library was electroporated into Escherichia coli cells (E. cloni 10G Elite; Lucigen, USA), yielding ≈ 107 colonies after overnight incubation on agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed and solubilised with 2000 U of Ready-Lyse Lysozyme (Epicentre) and 0.5% SDS (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from transfected cells using the QuickExtractTM DNA Extraction Solution (Lucigen) and mutations were detected by the SURVEYOR assay (Integrated DNA technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...