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Citations for Lucigen :
301 - 350 of 734 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... pure DNA was isolated by using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Illumina, San Diego, CA, USA) following the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... DNA was extracted directly from the sorted nuclei without centrifugation using the MasterPure DNA Extraction kit (Epicentre, Madison, Wisconsin, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... the Janus cassette was amplified from genomic DNA isolated from strain AZ59 (13) using the MasterPure DNA purification kit (Lucigen), with flanking regions (∼1kb ...
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bioRxiv - Microbiology 2022Quote: ... Cell samples were grown to mid-log in rich media and DNA was collected using a MasterPure Gram Positive DNA (Epicentre) purification kit with modifications as previously described [53] ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from 96-well plates of confluent clones using QuickExtract DNA Extraction Solution (Lucigen Corporation, Middleton, WI, USA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells from each of these 4 wells were dissociated and divided into 2 portions–one portion was used to collect genomic DNA using QuickExtract DNA Extraction solution (Lucigen) while 500 cells from the other portion was suspended in 25 ml of culture medium and 100 ul was seeded into each well of two 96-well plates for further culture ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA of Pkd1-/-or Pkd2-/- single cell clonal mNPC lines were extracted using QuickExtractTM DNA Extraction Solution (Lucigen, # QE09050). PCR primers were designed to flank the sgRNA targeting sites of Pkd1 or Pkd2 genes (listed below) ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the isolates using the MasterPure™ Gram positive DNA extraction kit (Cat. No. MGP04100; Epicentre®). The standard protocol was modified slightly to accommodate for the isolates being Gram negative organisms ...
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bioRxiv - Cell Biology 2023Quote: The electroporated progenitors were subjected to adipogenic differentiation for 8 days and the genomic DNA was isolated using QuickExtract DNA Extraction Solution (Lucigen). PCR reactions were conducted with 50 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using the Promega Maxwell Tissue DNA Kit with the addition of Readylyse Lysozyme Solution (Epicentre) and Lysostaphin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was prepared from 3 mL of turbid liquid culture with the MasterPureTM Gram Positive DNA Purification Kit (Lucigen). DNA was quantified by using a NanodropTM device (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 500uL of the cultured cells were pelleted and the growth media was removed. DNA was extracted using the Master Pure DNA purification kit (Cat No. 85200) from Epicentre. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L ...
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bioRxiv - Genetics 2023Quote: DNA was isolated from fixed and sorted cells using the MasterPure DNA purification kit (MC85200, Lucigen, LGC Ltd, Teddington, UK) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... the media was removed and genomic DNA was extracted using 30 uL/well of QuickExtract DNA Extraction Solution (Lucigen #QE09050) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Genomics 2020Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
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bioRxiv - Bioengineering 2019Quote: ... genomic DNA was extracted using QuickExtract Solution (Epicentre), and genomic loci were amplified by PCR utilizing the Phusion Hot Start Flex DNA Polymerase (NEB) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... QuickExtract DNA Extraction Solution was purchased from Lucigen Corporation (WI ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA samples were prepared using QuickExtract (Epicentre). The PCR products were column purified and analyzed with Surveyor Mutation Detection Kit (IDT) ...
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bioRxiv - Genomics 2020Quote: ... and NxSeq AmpFREE Low DNA Library Kit (Lucigen) was applied ...
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bioRxiv - Cell Biology 2021Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
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bioRxiv - Neuroscience 2020Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen) followed by PCR and Sequencing to determine efficient rescue.
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bioRxiv - Cancer Biology 2019Quote: ... DNA was isolated from clones with QuickExtract (Epicentre) for genotyping and replica plated for clonal expansion ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by chromosomal DNA isolation (Quick Extract /Lucigen), PCR amplification (Sup Table 8 ...
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bioRxiv - Microbiology 2020Quote: ... or MasterPure Gram Positive DNA purification kit (Lucigen). Sequencing libraries were prepared using NexteraXT (Illumina) ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: Genomic DNA was extracted with QuickExtract buffer (Lucigen) by adding 30 µL to a single well of a 96-well plate and incubating for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... repaired with End-IT DNA repair kit (Epicentre), A-tailed with Klenow enzyme (New England Biolabs) ...
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bioRxiv - Immunology 2020Quote: ... We digested the DNA with Quickextract (Lucigen Corporation) at 68°C for 90 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient repair of the mutation.
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bioRxiv - Microbiology 2022Quote: ... and DNA was extracted using GELase treatment (Epicentre Biotechnologies ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using Quickextract solution (Lucigen). A ∼700 bp-1 kb region flanking the sgRNA annealing site was amplified by PCR 5-7 days after RNP nucleofection ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted using QE buffer (Lucigen) and PCR was performed using Q5 polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the QuickExtract™ DNA Extraction Solution method (Lucigen), or a salt extraction method (Aljanabi & Martinez ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted with QuickExtract (Lucigen #QE09050) for amplicon sequencing.
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bioRxiv - Plant Biology 2023Quote: ... with NxSeq AmpFREE Low DNA Library Kits (Lucigen). After size selection by AMPureXP beads ...
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bioRxiv - Genetics 2023Quote: ... 4 units of Ampligase® DNA Ligase (Epicentre), 0.2 ul of Ampligase® 10X Reaction Buffer ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was isolated using QuickExtract solution (Lucigen). Reactions were incubated for 10 min at 65 °C followed by 5 min at 98 °C and cooling to 4 °C ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from clones following overnight culture using a MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corp., USA). The sequencing library was prepared using the Illumina Nextera kit following published methods (22 ...
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bioRxiv - Genetics 2021Quote: ... the genomic DNA of the pool of cells carrying insertion events was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Cells were lysed using a lysis solution supplemented in zymolyase 20T (1.5 mg/ml) ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Microbiology 2019Quote: ... 50 ml of culture was used for DNA extraction using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, WI, USA).
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bioRxiv - Molecular Biology 2021Quote: DNA was isolated from HTT Q71-GFP-expressing HEK293 cells through MasterPure™ Complete DNA and RNA Purification Kit (Epicentre®) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
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bioRxiv - Cancer Biology 2021Quote: ... One of the plates was used for quick genomic DNA isolation for each of the colonies using QuickExtract DNA extraction solution (Lucigen # QE09050). 5μl of this lysate was used to set up a quick genomic PCR screening for IL-1β knockout clones using checking primers ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...