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Citations for Lucigen :
551 - 600 of 734 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: DNA was isolated from each of the four cell pools with 100 µL QuickExtract (Lucigen) per 1 million cells following the manufacturer’s instructions ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The remaining linear DNA was treated by exonuclease (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K) at 37LJ°C for 1 week ...
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bioRxiv - Microbiology 2023Quote: ... each DNA sample was amplified in triplicate using the FailSafe™ PCR System (Epicentre, WI) and the 515FB-806RB primer pair to generate a 400 bp amplicon from the V4 variable regions of the 16S rRNA gene ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The end-repair reaction was done using the End-it DNA end-repair kit (Lucigen). P7 adapters were ligated to DNA fragments as described above ...
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bioRxiv - Genetics 2023Quote: ... 400 ng of DNA was incubated with 5 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Plant Biology 2024Quote: ... and then end-repair was performed by the End-It DNA End-Repair Kit (Epicentre). This end-repaired DNA was subjected to A-tailing using the Klenow 3′–5′ exo− enzyme (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
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bioRxiv - Genomics 2024Quote: ... the DNA & rRNA depleted RNA samples were treated with Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen). After a one-hour incubation ...
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bioRxiv - Biochemistry 2024Quote: ... The CRISPR/Cas9-edited mutants were validated by DNA extraction using QuickExtract solution (Lucigen, QER090150) followed by sanger sequencing prior cell line expansion28,38 ...
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bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
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bioRxiv - Cell Biology 2020Quote: RNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, MC85200), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
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bioRxiv - Microbiology 2019Quote: ... anthracis gDNA was isolated using the MasterPure Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI), a salt-precipitation (SP ...
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bioRxiv - Biochemistry 2019Quote: ... The human gDNA was isolated from U2OS cells using the QuickExtract™ DNA Extraction Solution (EPICENTRE). From the gDNA ...
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bioRxiv - Plant Biology 2021Quote: ... then further purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, USA). 100 ng of DNA was used for qPCR ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 ng each sample was subjected to end-repair (End-It DNA End-Repair kit, Lucigen), A-tailing (Klenow fragment (3’-5’ exo– ...
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bioRxiv - Microbiology 2022Quote: ... It was then purified using the MasterPure Gram Positive DNA purification kit (Lucigen, Middleton, Wisconsin, USA) using the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
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bioRxiv - Plant Biology 2019Quote: ... size-selected DNA fragments (150-350kb) were ligated into HindIII digested vector pIndigoBAC-5 (Epicentre, USA) and transformed into E ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from sorted and non-sorted cells using the QuickExtract reagent (Lucigen QE09050). In addition ...
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bioRxiv - Systems Biology 2020Quote: RFP positive A549 VIM-RFP cells were harvested and DNA was extracted using QuickExtract (Epicentre, QE09050). Primers were designed for left homology arm and right homology arm junction PCR (left junction Fwd ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... gargle solution or sputum sample were mixed 1:1 with 2x QuickExtract DNA extraction solution (Lucigen) and heat inactivated for 5 minutes at 95°C ...
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bioRxiv - Genetics 2020Quote: For single cell clones or bulk sequencing genomic DNA (gDNA) was extracted by quick extract (Lucigen). PCR amplification was performed with LongAmp Polymerase (NEB ...
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bioRxiv - Genomics 2021Quote: ... The libraries were generated using the NxSeq AmpFREE Low DNA Library Kit and NxSeq Adaptors (Lucigen). They were then sequenced on an Illumina HiSeq X (PE150).
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bioRxiv - Developmental Biology 2022Quote: ... End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25°C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Systems Biology 2023Quote: ... This pool of elements was end-repaired using End-It DNA End-Repair Kit (#ER0720; Epicentre) and self-ligated using Fast-link ligase (LK0750H ...
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bioRxiv - Biochemistry 2019Quote: ... Activity of the isolate was determined by comparing product DNA yields relative to a commercial isolate (Epicentre).
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bioRxiv - Genetics 2021Quote: ... genomic DNA from bulk cells of each transfected well was extracted using Quick Extract buffer (Lucigen #QE0905T). following the protocol published by the manufacturer (https://www.lucigen.com/docs/manuals/MA150E-QuickExtract-DNA-Solution.pdf) ...
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bioRxiv - Genetics 2019Quote: ... Another 10% of the cell culture was used for DNA extraction using 15 uL of QuickExtract (Lucigen). For the timecourse optimization experiment ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... jirovecii-infected patients was extracted using the MasterPure Yeast DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA). Total RNAs for P ...
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bioRxiv - Bioengineering 2020Quote: ... and had genetic material isolated via the MasterPure™ genomic DNA purification kit (Lucigen, Cat. No. MC85200). Genomic DNA was fragmented to 300 bp via Covaris sonication ...
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bioRxiv - Pathology 2021Quote: ... then gDNA was extracted using the MasterPure Gram Positive DNA Purification kit according to manufacturer’s protocol (Lucigen). Library preparation and sequencing was performed by the Microbial Genome Sequencing Center (MiGS) ...
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bioRxiv - Genomics 2020Quote: The gDNA used in this study was extracted with the MasterPure™ DNA Purification Kit (Epicentre, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... gDNA was directly isolated from conidia stocks using the MasterPure(tm) Yeast DNA Purification Kit (Lucigen/Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... gDNA was directly isolated from conidia stocks using the MasterPure(tm) Yeast DNA Purification Kit (Lucigen/Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... yolk sacs were dissected and a small fraction was placed in QuickExtract DNA Extraction Solution (Lucigen, QE09050) and processed according to manufacturer’s protocols.
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from flies using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) and converted to cDNA with the PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Developmental Biology 2022Quote: Tissue samples (ear and tail clips) from resulting animals were lysed using QuickExtract DNA Extraction Solution (Epicentre) followed by PCR at the specific genomic location ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal cell lines were isolated by single-cell sorting and characterized by genomic DNA extraction (Lucigen QuickExtract), PCR amplification of edited loci using Q5 polymerase (NEW ENGLAND BIOLABS ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA isolation was performed using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to manufacturer’s instructions and total gDNA was quantified using a nano-drop (Thermo-Fisher Scientific Waltham ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from individual female adult flies from stocks of interest using QuickExtract solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (Catalogue Number: 14000-1, Lucigen), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dsRNA was synthesized from the purified DNA using Ampliscribe T7-Flash Transcription kits (Epicentre Technologies, Co., Wisconsin, USA). We designed the PCR primers using the Primer3Web version 4.1.0 (Untergasser et al ...
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bioRxiv - Genomics 2020Quote: ... Bisulfite-converted DNA was used for single-stranded library preparation using the EpiGnome Methyl-Seq kit (Epicentre, EGMK81312) with the described modifications ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...