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Citations for Lucigen :
401 - 450 of 759 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... it was extracted (QuickExtract DNA extraction solution, Epicentre Biotechnologies), the region of interest was amplified by PCR (primers described in Supplemental Table 4) ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using “Yeast Masterpure kit” (Epicentre, USA) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: Total DNA was extracted using a QuickExtract kit (Lucigen). Unless otherwise indicated ...
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bioRxiv - Genetics 2020Quote: ... and a MasterPure™ Yeast DNA Purification Kit (Lucigen). PE150 libraries of the evolved strains were prepared and sequenced by Novogen.
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bioRxiv - Microbiology 2021Quote: ... swinhoei Y total DNA in the pWEB vector (Epicentre), was screened and a 3D growth-and-dilution library format as reported earlier45 ...
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bioRxiv - Genomics 2022Quote: ... 16 U of DNA Ampligase (Epicentre Technologies, Madison, WI), 0.016 mM dNTP ...
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bioRxiv - Genomics 2022Quote: ... 1x Ampligase DNA ligase buffer (Epicentre Technologies, Madison, WI), 0.5 μl of 5M Betain (Sigma Aldrich) ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using the MasterPure kit (Lucigen, MC85200), and stored at 4C until use ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA for clone screens was extracted by QuickExtract (Lucigen) or ethanol precipitation.
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bioRxiv - Genetics 2020Quote: ... DNA was isolated using the QuickExtract kit (Epicentre, #QE09050) and the target region in the last exon of ACTN2 (Primers ...
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bioRxiv - Genetics 2020Quote: ... Worms were lysed in DNA Quick Lysate (Epicentre Technologies) for 1 hour at 60°C and the lysate was then used as a template for PCR with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... using Masterpure complete DNA and RNA purification kit (Lucigen), genomic DNA for whole genome sequencing was isolated ...
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bioRxiv - Neuroscience 2022Quote: 30 µl of Quick Extract DNA Estraction (Lucigen, QE09050,) was added to 5.0 x 104 pelleted iPSC ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was extracted using the DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient mutation rescue.
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bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or the Epicentre MasterPure DNA Purification Kit (Epicentre Biotechnologies). All plasmid constructs were built with the Gibson Assembly Master Mix or the NEB Builder HiFi DNA Assembly (both New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... 1µl Fast-Link DNA ligase (Lucigen, cat. no. LK6201H), 3 µl 10× Fast link ligation buffer and 1mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: QuickExtract DNA Extraction Solution (Lucigen, cat. No. LGN-QE09050)
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bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
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bioRxiv - Immunology 2021Quote: 200ul aliquots of blood samples collected on GD15 were used for genomic DNA (gDNA) extraction for bisulfite pyrosequencing using MasterPure DNA Purification kits (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Microbiology 2021Quote: ... two ml of a culture grown in R2B were used for extraction of high molecular weight (HMW) DNA using the MasterPureTM DNA Purification Kit (Epicentre, Madison, WI, USA), using the kit’s protocol for cell samples ...
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bioRxiv - Bioengineering 2022Quote: Samples collected at 24 h were processed for DNA isolation using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA from cell lines growing on 96-well plates was extracted by 25 µl QuickExtract DNA extraction solution (Epicentre #QE09050, Madison, WI, USA), further diluted to 200 µl with H2O ...
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bioRxiv - Genomics 2020Quote: ... or homogenized tissues were digested with proteinase K prior to DNA isolation using MasterPure™ DNA Purification Kit (Epicentre, by Illumina, Madison, United States) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Isolation of genomic DNA from leaf powder was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre (Epicentre, Chicago, USA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was end-repaired using End-it from Lucigen. 17.5ul DNA was added to 2.5ul 10X end-it buffer ...
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bioRxiv - Microbiology 2019Quote: ... NxSeq DNA sample prep kit 2 (Lucigen, Middleton, WI, USA) was used as per manufacturer’s directions with either NEXTFlex 48 barcodes (BioO ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was isolated from obtained clones using QuickExtract (Lucigen), the sgRNA-targeted sites PCR amplified and the products Sanger-sequenced ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from obtained clones using QuickExtract (Lucigen), the gRNA-targeted sites PCR amplified and the products Sanger-sequenced ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was electroporated into Endura competent cells (Lucigen) using 50-100ng DNA per electroporation (we set up 6 or 24 replicate transformation for tiling or genome-wide sgRNA library to maintain library complexity) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted following the protocol amended from Epicentre MasterPure™ DNA Purification Kit (http://homings.forsyth.org/DNA%20Isolation%20Protocol.pdf) ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed using QuickExtract DNA Extraction Solution (Lucigen) following supplier’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... genomic DNA was extracted with the QuickExtract kit (Epicentre, #QE09050). PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Genetics 2019Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Genomics 2019Quote: ... The DNA was inserted into the vector pIndigoBAC-5 (Epicentre, Madison ...
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bioRxiv - Genomics 2019Quote: ... and linear DNA was digested by plasmid safe (Epicentre, Illumina) at 37°C for 1.5 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... The DNA was then reacted with EZ-Tn5 transposase (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was extracted from HEP3B cells using QuickExtract (Epicentre).
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bioRxiv - Microbiology 2020Quote: ... Any remaining DNA was degraded via Baseline-ZERO-DNase (Epicentre) using manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted with Master Pure purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Neuroscience 2022Quote: ... were ligated to the ChIP DNA (Fast-Link kit; Epicentre) and PCR amplified using the Illumina TruSeq ChIP Library Prep Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA was obtained by using QuickExtract DNA Extraction Solution (Lucigen), where 10000 cells were resuspended in 100 μl PBS ...