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Citations for Lucigen :
601 - 650 of 759 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... It was then purified using the MasterPure Gram Positive DNA purification kit (Lucigen, Middleton, Wisconsin, USA) using the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2019Quote: ... size-selected DNA fragments (150-350kb) were ligated into HindIII digested vector pIndigoBAC-5 (Epicentre, USA) and transformed into E ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from sorted and non-sorted cells using the QuickExtract reagent (Lucigen QE09050). In addition ...
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bioRxiv - Systems Biology 2020Quote: RFP positive A549 VIM-RFP cells were harvested and DNA was extracted using QuickExtract (Epicentre, QE09050). Primers were designed for left homology arm and right homology arm junction PCR (left junction Fwd ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... gargle solution or sputum sample were mixed 1:1 with 2x QuickExtract DNA extraction solution (Lucigen) and heat inactivated for 5 minutes at 95°C ...
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bioRxiv - Genetics 2020Quote: For single cell clones or bulk sequencing genomic DNA (gDNA) was extracted by quick extract (Lucigen). PCR amplification was performed with LongAmp Polymerase (NEB ...
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bioRxiv - Genomics 2021Quote: ... The libraries were generated using the NxSeq AmpFREE Low DNA Library Kit and NxSeq Adaptors (Lucigen). They were then sequenced on an Illumina HiSeq X (PE150).
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bioRxiv - Developmental Biology 2022Quote: ... End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25°C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Systems Biology 2023Quote: ... This pool of elements was end-repaired using End-It DNA End-Repair Kit (#ER0720; Epicentre) and self-ligated using Fast-link ligase (LK0750H ...
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bioRxiv - Biochemistry 2019Quote: ... Activity of the isolate was determined by comparing product DNA yields relative to a commercial isolate (Epicentre).
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bioRxiv - Genetics 2021Quote: ... genomic DNA from bulk cells of each transfected well was extracted using Quick Extract buffer (Lucigen #QE0905T). following the protocol published by the manufacturer (https://www.lucigen.com/docs/manuals/MA150E-QuickExtract-DNA-Solution.pdf) ...
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bioRxiv - Genetics 2019Quote: ... Another 10% of the cell culture was used for DNA extraction using 15 uL of QuickExtract (Lucigen). For the timecourse optimization experiment ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... jirovecii-infected patients was extracted using the MasterPure Yeast DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA). Total RNAs for P ...
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bioRxiv - Bioengineering 2020Quote: ... and had genetic material isolated via the MasterPure™ genomic DNA purification kit (Lucigen, Cat. No. MC85200). Genomic DNA was fragmented to 300 bp via Covaris sonication ...
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bioRxiv - Pathology 2021Quote: ... then gDNA was extracted using the MasterPure Gram Positive DNA Purification kit according to manufacturer’s protocol (Lucigen). Library preparation and sequencing was performed by the Microbial Genome Sequencing Center (MiGS) ...
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bioRxiv - Genomics 2020Quote: The gDNA used in this study was extracted with the MasterPure™ DNA Purification Kit (Epicentre, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... gDNA was directly isolated from conidia stocks using the MasterPure(tm) Yeast DNA Purification Kit (Lucigen/Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... gDNA was directly isolated from conidia stocks using the MasterPure(tm) Yeast DNA Purification Kit (Lucigen/Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... yolk sacs were dissected and a small fraction was placed in QuickExtract DNA Extraction Solution (Lucigen, QE09050) and processed according to manufacturer’s protocols.
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from flies using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) and converted to cDNA with the PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Developmental Biology 2022Quote: Tissue samples (ear and tail clips) from resulting animals were lysed using QuickExtract DNA Extraction Solution (Epicentre) followed by PCR at the specific genomic location ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal cell lines were isolated by single-cell sorting and characterized by genomic DNA extraction (Lucigen QuickExtract), PCR amplification of edited loci using Q5 polymerase (NEW ENGLAND BIOLABS ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA isolation was performed using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to manufacturer’s instructions and total gDNA was quantified using a nano-drop (Thermo-Fisher Scientific Waltham ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from individual female adult flies from stocks of interest using QuickExtract solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (Catalogue Number: 14000-1, Lucigen), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dsRNA was synthesized from the purified DNA using Ampliscribe T7-Flash Transcription kits (Epicentre Technologies, Co., Wisconsin, USA). We designed the PCR primers using the Primer3Web version 4.1.0 (Untergasser et al ...
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bioRxiv - Genomics 2020Quote: ... Bisulfite-converted DNA was used for single-stranded library preparation using the EpiGnome Methyl-Seq kit (Epicentre, EGMK81312) with the described modifications ...
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bioRxiv - Bioengineering 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed in 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) using a SimpliAmp Thermal Cycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dsRNA was synthesized from the purified DNA using Ampliscribe T7-Flash Transcription kits (Epicentre Technologies, Co., Wisconsin, USA). We designed the PCR primers using the Primer3Web version 4.1.0 (Untergasser et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed in 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) using a SimpliAmp Thermal Cycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligated DNA was packaged into virions using the MaxPlax λ packaging extract (Lucigen #MP5120, Middleton, WI, USA), and the virions were sequenced to determine input barcode frequency.
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bioRxiv - Evolutionary Biology 2019Quote: ... and size-selection was performed using the Lucigen NxSeq® AMPFree Low DNA Library Kit (Lucigen, Middleton, WI). Libraries were quantified using a Qubit 2.0 instrument (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... Libraries for WGBS were prepared as follows: DNA fragments were end-repaired using the End-It kit (Epicentre), A-tailed with Klenow exo-(NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA template was incubated at 37°C overnight in a transcription mix containing: transcription buffer 1X (Epicentre Biotechnologies), 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Immunology 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Clones were identified ∼2 weeks after sorting and screened by genomic DNA extraction with Quickextract (Lucigen cat.#QE09050) and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712) ...
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bioRxiv - Biochemistry 2021Quote: ... Linear PCR products were ligated by blunt-end ligation using the Fast-link DNA ligation kits from Epicentre. 5μl of the ligation reaction were used for the transformation of chemically-competent E ...
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bioRxiv - Microbiology 2019Quote: ... All gDNA samples were cleaned to remove the remaining RNA using the Genomic DNA Clean & Concentrator kit (Epicentre) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5% SDS) and purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, WI, USA). RNA was prepared by TRizol according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.