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Citations for Lucigen :
351 - 400 of 777 citations for ssc mir 17 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The DNA library was prepared using a NxSeq AmpFREE Low DNA Library kit (Lucigen) and sequenced on a Illumina HiSeq2000 with a 100bp paired-end mode ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the ScriptSeq™ Complete Kit (Epicentre, Madison, WI, USA) according to the manu-facturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction from pellets was done using MasterPure™ DNA purification kit (Epicentre, US) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted using the Ribo-Zero™ Kit (Epicentre, Madison, WI, USA). The viral dsRNA of these strains was extracted and prepared as previously described [22] ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted using Lucigen Kit as per manufacturer’s instructions (Bioresearch, Lucigen, UK) and samples were sequenced by Novagene (Cambridge ...
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bioRxiv - Microbiology 2022Quote: ... Preparation of genomic DNA used the MasterPure™ Yeast DNA Purification Kit (Lucigen # MPY80200) with a NextSeq500– 1flowcell mid output (130M fragments) ...
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions and stored in RNase-free Tris-EDTA buffer ...
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre, Madison WI) and RNA purity and concentration were confirmed using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... The dsRNA was synthesized using the AmpliScribe T7 High Yield Transcription Kit (Lucigen, USA). Primer sequences for the PCR amplification are provided in Supplementary Table S3.
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bioRxiv - Genetics 2024Quote: ... Total DNA was purified using Epicentre Masterpure™ Yeast DNA Purification Kit (Lucigen, USA) and resuspended in 50 µL of RNase-free water ...
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using the MasterPureTM Complete DNA and RNA Purification Kit (Lucigen, USA), including an additional bead-beating step using a FastPrep-24TM ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). Quantitative PCR (qPCR ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using Lucigen MasterPure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). DNA concentration was measured by bio-spectrophotometer absorbance readings and diluted to desired target concentrations.
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Bioengineering 2023Quote: ... We synthesized mRNA using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, catalog #ASF-3507) as described previously.(79 ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was treated with the Ribo-Zero Gold kit (Human/Mouse/Rat, Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... ribosomal RNA (rRNA) was removed using the Ribo-Zero Magnetic Kit for bacteria (Epicentre, IL). Metatranscriptome library preparation was performed using the Ion Total RNA-Seq Kit v2 (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA extraction was performed using the MasterPure Yeast DNA Purification Kit (Epicentre, United States) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNAse-treated RNA (5 µg) was mRNA enriched using a Ribo-Zero Magnetic Kit (Epicentre). After Illumina sequencing the reads were mapped to C ...
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bioRxiv - Molecular Biology 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Genomics 2020Quote: ... and rRNA depletion was performed using Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina).
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bioRxiv - Genomics 2019Quote: ... The resulting template was transcribed using the Lucigen AmpliScribe T7-flash Transcription Kit from Epicentre/Illumina (cat ...
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bioRxiv - Plant Biology 2019Quote: ... RNA-seq library construction was carried out with the ScriptSeq™ v2 kit (Epicentre SSV21124). Final libraries were amplified with 13 PCR cycles ...
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bioRxiv - Genomics 2019Quote: ... genomic DNA samples (∼2 µg) were end-repaired using the End-it kit from Epicentre Technologies (Madison ...
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bioRxiv - Genomics 2019Quote: ... undigested genomic DNA was end-repaired using the End-ItTM kit (Epicentre Technologies, Madison, WI). The enzyme was removed by phenol:chloroform:isoamyl alcohol (PCI ...
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bioRxiv - Microbiology 2019Quote: ... 200 ng of template DNA were transcribed using the AmpliScirbe T7 Flash Transcription Kit (Epicentre) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from pelleted cells using a MasterPure Yeast RNA extraction kit (Lucigen, MPY03100), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... bacterial genomic DNA was prepared using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen) and fragmented by AatII digestion and the blot was probed with btl19-13 ...
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bioRxiv - Bioengineering 2020Quote: ... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... PP contamination was removed using the Plasmid Safe ATP-dependent DNase kit (Epicentre, WI, USA), and the products where cleaned and concentrated using the Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Systems Biology 2021Quote: ... thermophilus HB8 was isolated using the MasterPure Complete DNA purification Kit (Lucigen, Middleton, Wisconsin, US) and used as template for the amplification of the dxs (1-deoxy D-xylulose 5-phosphate synthase ...
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bioRxiv - Genetics 2020Quote: ... and RNA was extracted from each replicate using the MasterPure Yeast RNA Purification Kit (Epicentre). Integrity ...
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bioRxiv - Microbiology 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Systems Biology 2020Quote: ... and removed stable RNAs by using the RiboZero Gram Positive ribosomal RNA depletion kit (Epicentre). We prepared the sequencing libraries using the TruSeq stranded Total RNA kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... high molecular weight DNA was isolated using the MasterPure DNA Purification kit (Epicentre, no. MC85200). Sequencing was performed using the PacBio Sequel (Pacific Biosciences ...
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bioRxiv - Genomics 2022Quote: ... the clean-up DNAs underwent the protocol SYGNIS TruePrimeTM WGA & Single Cell WGA Kits (Lucigen), instead of the REPLI-g single cell kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification and precipitation of RNA were performed using the MasterPure RNA Purification Kit from Epicentre by adding 200 μl of 2X T and C Lysis Solution and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Random knockout mutants were produced using the EZ::Tn5Tm
Tnp TransposomeTm kit (Epicentre) following the manufacturers recommendations ... -
bioRxiv - Microbiology 2020Quote: ... Germany). MasterPure DNA purification kit (Cat. No. MCD85201) was obtained from Epicentre (Middleton, WI, USA). GoTaq Green Master Mix (Cat ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the Quantseq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in two steps ...
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bioRxiv - Molecular Biology 2020Quote: ... The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs) ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was extracted using the MasterPure yeast DNA purification kit (Epicentre Biotechnologies, Madison, WI) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... and in vitro transcription performed using the T7-Flash BiotinRNA Transcription Kit (Epicentre, biotin labelling) or TranscriptAid T7 High Yield Transcription Kit (ThermoScientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... shRNAs were synthesized with AmpliScribe™ T7-Flash™ Transcription Kit (Epicentre, Charlotte, NC, USA) for 15 h followed by 15 min treatment with 1 μl of DNAse I ...