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Citations for Lucigen :
551 - 600 of 777 citations for ssc mir 17 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... rRNA was depleted from the sample using the Gram-negative bacteria Ribo-Zero rRNA Removal Kit (Illumina-Epicentre). RNA-seq libraries were prepared with an Illumina TruSeq stranded RNA kit according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Libraries for WGBS were prepared as follows: DNA fragments were end-repaired using the End-It kit (Epicentre), A-tailed with Klenow exo-(NEB ...
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bioRxiv - Cancer Biology 2019Quote: We removed rRNA from extracted total RNA using a Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA) and then performed library preparation using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Molecular Biology 2019Quote: ... 5µg of total RNA was subjected to rRNA depletion using the RiboZero Human/Mouse/Rat kit (Epicentre Biotechnologies). cDNA was generated from the depleted RNA using random hexamers or custom primers and Superscript III (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: DNA from the oral samples were extracted using the MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI). 2 ml TE-buffer containing the sample material was pelleted by centrifugation (10000 rpm for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal Kit (Epicentre/Illumina, Human/Mouse/Rat) and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of total RNA were depleted of ribosomal RNA using the Ribo-Zero rRNA removal kit (Epicentre). The resulting RNA was then used for library preparation using the TruSeq small RNA Sample Prep Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA from the cell pellets were extracted using a MasterPure™ DNA Extraction kit (Epicentre®, Madison, USA) according to the manufacturer’s protocol ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... sgRNA was synthesized in vitro as per the suggested protocol using Ampliscribe T7 Flash Transcription kit (Lucigen, USA). 6xHis-Cas9 protein was synthesized as described previously25 ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA fragments were then blunted and phosphorylated using End-It DNA End-Repair Kit (#ER0720; Epicentre). Part of the repaired pool was set apart for cloning of singlet libraries ...
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bioRxiv - Microbiology 2021Quote: ... Transposon mutagenesis was performed with the EZ-Tn5
Tnp Transposome kit (Epicentre, Illumina, CA, USA) and the insertion site of all mutants was determined via arbitrary PCR (Segev et al ... -
bioRxiv - Microbiology 2019Quote: ... All gDNA samples were cleaned to remove the remaining RNA using the Genomic DNA Clean & Concentrator kit (Epicentre) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was subjected to rRNA-depletion using RiboZero™ rRNA Removal Kit (EpiCentre Inc., Madison, WI, USA). Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeq™ v2 RNA-Seq Library Preparation guide (EpiCentre Inc. ...
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bioRxiv - Genetics 2022Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μg of RNA was treated with a beta version of Ribo-Zero Magnetic Gold Kit Yeast (Epicentre) to deplete rRNAs ...
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bioRxiv - Microbiology 2024Quote: ... RNA clean-up and library prep were performed using either the Ribo-Zero(TM) rRNA Removal Kit (Epicentre) with the Illumina Truseq Stranded RNA LT kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and bacterial DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5% SDS) and purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, WI, USA). RNA was prepared by TRizol according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted from yeast pellets using the MasterPure™ Yeast RNA Purification Kit (Lucigen Cat. No. MPY03100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
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bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and derived keratinocytes from three patients (11 total samples) with the MasterPure Complete DNA Purification Kit (Lucigen #MC85200). The gDNA yield was quantified by the Qubit dsDNA high-sensitivity (HS ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was isolated from the thawed cell pellets using the MasterPure Yeast DNA Purification kit (Lucigen MPY80200). The DNA was digested with XhoI and NgoMIV and fractionated on a 0.6% agarose gel ...
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bioRxiv - Cell Biology 2019Quote: ... were grown overnight at 37°C with 12.5 µg of chloramphenicol and extracted using a BACMAX DNA purification kit (Epicentre). Then ...
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bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...
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bioRxiv - Immunology 2021Quote: ... libraries were constructed from first strand cDNA using ScriptSeqTM v2 RNA-Seq library preparation kit (Epicentre Biotechnologies, Madison, WI). Briefly ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a (modified) CTAB protocol or a rapid desalting method (MasterPure™ Complete DNA and RNA Purification Kit; Lucigen Corporation).
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bioRxiv - Genomics 2020Quote: ... an aliquot of 25 uL was reserved from each sample for extraction of DNA with MasterPure™ kit (Epicentre) and bacterial cell count was measured using qPCR (TaqMan™) ...
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bioRxiv - Genomics 2020Quote: ... gDNA for the rest of the strains was extracted using the Masterpure Complete DNA and RNA purification kit (Lucigen) using the protocol for tissue samples ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and shSmt3 GLKD lines was depleted of ribosomal RNA with the Ribo-Zero™ rRNA Removal Kit (Epicentre/Illumina). Initial RNA-seq libraries were made using the TruSeq RNA prep kit by Illumina (shW ...
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bioRxiv - Microbiology 2019Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed using the Experion RNA Analysis Kit (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... About 30 ug of total gDNA was recovered from using MasterPure™ Complete DNA and RNA Purification kit (Lucigen), and 0.5ug-1ug gDNA was checked on a 1% agarose gel for integrity and quality ...
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Barcoding and demultiplexing Oxford Nanopore native RNA sequencing reads with deep residual learningbioRxiv - Genomics 2019Quote: ... Curlcake in vitro transcribed (IVT) sequences were produced using the Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). Sequin IVT sequences were produced using SP6 Polymerase (NEB-M0207S) ...
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bioRxiv - Microbiology 2021Quote: Phage DNA extractions were performed from high titer suspensions using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Stranded RNA-seq libraries were prepared from 1 μg total RNA using ScriptSeq v2 Complete kit for bacteria (Epicentre) and sequenced on Illumina NovaSeq 6000 platform at GENEWIZ ...
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bioRxiv - Neuroscience 2019Quote: ... 20pg of in vitro transcribed RNA synthesized from the T7 control DNA Template (AmpliScribe™ T7 Transcription Kit, Epicentre) was added to the samples ...
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bioRxiv - Molecular Biology 2020Quote: The initial RNA library was transcribed from 1µg of input dsDNA using the AmpliScribe T7-Flash Transcription Kit (Epicentre). 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Road Madison, WI) with a total amount of 2 ug RNA as an input for each library ...
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Epigenetics of post-operative delirium: A genome-wide DNA methylation study of neurosurgery patientsbioRxiv - Neuroscience 2022Quote: Genomic DNA was extracted from whole blood tissues with the MasterPure DNA extraction kit (MCD85201, Epicentre, Madison, WI, USA) following the recommended protocol ...
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bioRxiv - Genomics 2022Quote: DNA was isolated from the 16 parental strains using the Masterpure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). The Pacific Biosciences protocol “Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” was used to create libraries from 30 micrograms of DNA ...
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bioRxiv - Genomics 2022Quote: High molecular weight DNA was extracted using either the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), or by standard phenol/chloroform extraction ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a rolling-circle amplification (RCA) reaction mixture (1 unit/μL phi29 polymerase (Lucigen, NxGen kit 30221), 1x phi29 polymerase buffer ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from 50mL flash-frozen cell pellets grown to mid-log (OD600 ∼0.65-0.85) using MasterPure™ yeast RNA purification kit (Lucigen) including a DNaseI treatment step ...
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bioRxiv - Microbiology 2022Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed on a Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA of the single cell clones with good proliferation was extracted using a QuickExtract DNA extraction kit (Epicentre). The exon 23-24 region was amplified by a pair of PCR primer (SMCHD1_PCR ...