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Citations for Lucigen :
301 - 350 of 744 citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... rRNA was removed with the Ribo-Zero rRNA Removal Kit (MRZMB 126, Epicentre Biotechnologies). For cDNA library construction ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted using Epicentre MasterPure Yeast RNA Purification kit (Epicentre, Madison, WI, USA) and stored in RNase-free TE buffer ...
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bioRxiv - Genetics 2020Quote: ... The genomic DNA extracted using MasterPure Yeast DNA Purification Kit (tebu-bio: now Lucigen) was sequenced using Illumina HiSeq 2000 technology with 100 bp paired-end libraries ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from mouse tails using the QuickExtract DNA extraction kit (Epicentre) following manufacturer’s instructions ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... The size-selected and purified cDNA was circularized using CircLigase ssDNA ligase kit (Lucigen) following manufacture’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... cDNA libraries were generated using a strand-specific ScriptSeq Complete Kit Bacteria protocol (Epicentre), indexed and sequenced on an Illumina HiSeq 2000 platform at the Lausanne Genomics Facilities with 101-nt single-end reads ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated with the MasterPure™ Yeast RNA Purication Kit (Epicentre MPY03100). For RNA sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and RNA extracted with MasterPure™ Yeast RNA Purification Kit (Epicentre). Samples were vacuum dried and sent to Genewiz (South Plainfield ...
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bioRxiv - Bioengineering 2022Quote: 2’-Fluoro ssRNA was produced by IVT using the Durascribe T7 IVT kit (Epicentre). Reactions consisted of 0.2-1 μg of purified DNA template per 20 μl reaction ...
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bioRxiv - Genomics 2022Quote: ... Repair ends of DNA using End-It DNA End-Repair Kit (Lucigen, Cat. ER0720), then perform A-tailing with Klenow Fragment (3’-> 5’ exo- ...
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bioRxiv - Microbiology 2022Quote: ... DNA was prepared using the MasterPure™ Complete DNA and RNA purification kit (Lucigen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was removed using a Ribo-Zero rRNA removal kit (Epicentre; Madison, WI). Gene expression was quantified through the Kallisto software (Bray et al. ...
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bioRxiv - Genomics 2022Quote: ... Sonicated gDNA ends were repaired using End-It™ DNA End-Repair Kit (Epicentre) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated using MasterPure complete DNA and RNA purification kit (Epicentre, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli genomic DNA was purified using the MasterPure Gram Positive DNA Purification Kit (Epicentre). All DNAs were purified again using the Zymo Clean and Concentrator-25 (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing libraries were generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) using 12 PCR cycles for amplification ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was prepared using the MasterPure complete DNA and RNA purification kit (Epicentre) and treated with MmeI and the calf intestinal phosphatase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: Yeast total RNA samples were prepared by MasterPure Yeast RNA extraction kit (Lucigen, MPY03100). For mouse ESCs cells ...
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bioRxiv - Genomics 2020Quote: ... Yeast total RNA was extracted with the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... BACs were isolated from 50ml bacterial cultures using the BACMAX Bac extraction kit (Epicentre). Isolated BACs were sonicated (x10 cycles of 15 seconds ‘on’ 45 seconds ‘off’ at constant intensity with power set to ‘3’ ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the Ribo-Zero Gold kit (Epicentre). Ribosome-depleted RNA was used to create multiplexed RNA-seq libraries (NEBNext Ultra ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from candidate mutants with the MasterPure DNA purification kit (Epicentre) and PCR followed by gel electrophoresis confirmed correct integration of the NAT dominant resistance marker ...
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bioRxiv - Molecular Biology 2020Quote: ... and ribosomal RNA was depleted using the Ribo-Zero Plant rRNA removal kit (Epicentre). chrRNA-seq libraries were constructed from 50 ng of purified RNA using the KAPA Stranded mRNA-seq Kit ...
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bioRxiv - Microbiology 2019Quote: ... aureus samples by following manufacturer’s instructions (MasterPure gram positive DNA Purification Kit; Lucigen, USA). Briefly ...
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bioRxiv - Genetics 2019Quote: ... Total RNAs were depleted of ribosomal RNA with Ribo-Zero rRNA Removal kit (Epicentre). Libraries for sequencing ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using the MasterPure RNA Purification Kit from Epicentre following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The bisulfite-modified DNA-sequencing library was generated using the EpiGnome™ kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Residual Ribosomal RNA (rRNA) was depleted using Ribo-Zero™ kits (Epicentre, Madison, WI) before library construction ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was prepared using the MasterPure™ Yeast DNA purification kit from Lucigen. To determine the MAT ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA was extracted using MasterPure Complete DNA & RNA Purification Kits (Lucigen Simplifying Genomics, USA) according to the manufacturer’s protocol and diluted to 50 ng/μL.
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted using a MasterPure™ Yeast RNA Purification Kit (Lucigen MPY03100), the RNA concentration was determined on a Nanodrop 2000C (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... respectively) had their total genomic DNA extracted using MasterPure Yeast DNA Purification Kit (Lucigen), with cell lysis solutions and RNAse A treatment volumes scaled up 4x to adequately lyse all cells and remove RNA ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA library was prepared using a NxSeq AmpFREE Low DNA Library kit (Lucigen) and sequenced on a Illumina HiSeq2000 with a 100bp paired-end mode ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the ScriptSeq™ Complete Kit (Epicentre, Madison, WI, USA) according to the manu-facturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction from pellets was done using MasterPure™ DNA purification kit (Epicentre, US) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted using the Ribo-Zero™ Kit (Epicentre, Madison, WI, USA). The viral dsRNA of these strains was extracted and prepared as previously described [22] ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted using Lucigen Kit as per manufacturer’s instructions (Bioresearch, Lucigen, UK) and samples were sequenced by Novagene (Cambridge ...
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bioRxiv - Microbiology 2022Quote: ... Preparation of genomic DNA used the MasterPure™ Yeast DNA Purification Kit (Lucigen # MPY80200) with a NextSeq500– 1flowcell mid output (130M fragments) ...
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions and stored in RNase-free Tris-EDTA buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using the MasterPureTM Complete DNA and RNA Purification Kit (Lucigen, USA), including an additional bead-beating step using a FastPrep-24TM ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). Quantitative PCR (qPCR ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using Lucigen MasterPure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). DNA concentration was measured by bio-spectrophotometer absorbance readings and diluted to desired target concentrations.
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Bioengineering 2023Quote: ... We synthesized mRNA using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, catalog #ASF-3507) as described previously.(79 ...
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre, Madison WI) and RNA purity and concentration were confirmed using a Bioanalyzer 2100 (Agilent ...