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Citations for Lucigen :
101 - 150 of 774 citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The WT NPSL2 DNA template was amplified via polymerase chain reaction (PCR) using a set of four primers (Table S4) with EconoTaq PLUS 2x Master Mix (Lucigen). All the primers were ordered from Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Single clones were expanded and genotyped for the desired knock-in by PCR on genomic DNA extracted with Quickextract solution (Lucigen). Clones showing biallelic knock-ins were expanded and frozen in LN2 at early passages ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Biophysics 2023Quote: The templates for preparation of the extended pre-miR-31 for DMS-MaPseq and FL pre-miR-31 for NMR studies were generated by overlap-extension (OE) polymerase chain reaction (PCR) using EconoTaq PLUS 2x Master Mix (Lucigen) with primers listed in Tables S6 and S7 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Biochemistry 2024Quote: DNA template for the SreA aptamer domain was generated by PCR amplification of the HH SreA pUC57 plasmid with EconoTaq PLUS 2x Master Mix (Lucigen) using primers that are complementary to the 5′ and 3′ ends of P1 (Table S3) ...
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bioRxiv - Genomics 2020Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and the libraries have been generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genomics 2020Quote: ... and NxSeq AmpFREE Low DNA Library Kit (Lucigen) was applied ...
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bioRxiv - Genetics 2022Quote: ... and the MasterPureTM Yeast RNA Purification Kit (Epicentre). cDNAs were synthesized with the FastQuant RT Kit (with gDNase ...
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bioRxiv - Cell Biology 2021Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
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bioRxiv - Microbiology 2020Quote: ... or MasterPure Gram Positive DNA purification kit (Lucigen). Sequencing libraries were prepared using NexteraXT (Illumina) ...
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bioRxiv - Cell Biology 2019Quote: ... using the ScriptSeq kit (Epicentre, CA, USA SS10906). Libraries were amplified via polymerase chain reaction for 12–15 cycles and sequenced in two lanes on the HiSeq 2000 platform at BGI Genome Center (Shenzhen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... repaired with End-IT DNA repair kit (Epicentre), A-tailed with Klenow enzyme (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... with NxSeq AmpFREE Low DNA Library Kits (Lucigen). After size selection by AMPureXP beads ...
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bioRxiv - Evolutionary Biology 2024Quote: The AmpliScribe T7 High Yield Transcription kit (Lucigen) was used for RNA in vitro transcription with a 2-hour incubation at 37°C using gblock sequences available in supplemental ...
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bioRxiv - Microbiology 2024Quote: ... RNA extracted using MasterPure RNA Purification Kit (Epicentre), and treated with TURBO DNase as described ...
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bioRxiv - Genomics 2022Quote: ... and three libraries for each sex were generated with the NEBNext Ultra RNA library prep kit combined with rRNA depletion via the Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies). Samples were sequenced on an Illumina HiSeq2500 platform at the Vienna BioCenter Core Facilities (VBCF ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Ribo Zero kit and samples were converted into Illumina sequencing libraries using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina). Libraries were sequenced using Illumina HiSeq (2 × 150 bp reads) ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using “Yeast Masterpure kit” (Epicentre, USA) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: Total DNA was extracted using a QuickExtract kit (Lucigen). Unless otherwise indicated ...
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bioRxiv - Genetics 2020Quote: ... and a MasterPure™ Yeast DNA Purification Kit (Lucigen). PE150 libraries of the evolved strains were prepared and sequenced by Novogen.
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bioRxiv - Molecular Biology 2020Quote: ... we used the MasterPure RNA purification kit from Epicentre instead of phenol/chloroform extraction to purify RNA after the dephosphorylation and ligation steps.
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using the MasterPure kit (Lucigen, MC85200), and stored at 4C until use ...
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bioRxiv - Genetics 2020Quote: ... DNA was isolated using the QuickExtract kit (Epicentre, #QE09050) and the target region in the last exon of ACTN2 (Primers ...
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bioRxiv - Genetics 2020Quote: ... using Masterpure complete DNA and RNA purification kit (Lucigen), genomic DNA for whole genome sequencing was isolated ...
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bioRxiv - Plant Biology 2020Quote: ... Ribo-Zero kit (Epicentre, an Illumina company, Madison, WI) was used to remove rRNA from the libraries ...
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bioRxiv - Microbiology 2021Quote: The ScriptSeq complete kit (Epicentre, Illumina, Madison, WI USA), a combined kit for the ribosomal (rRNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or the Epicentre MasterPure DNA Purification Kit (Epicentre Biotechnologies). All plasmid constructs were built with the Gibson Assembly Master Mix or the NEB Builder HiFi DNA Assembly (both New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
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bioRxiv - Molecular Biology 2023Quote: Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina) was used to remove ribosomal RNAs and to enrich mRNAs ...
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bioRxiv - Biochemistry 2023Quote: ... AmpliScribe™ T7-Flash™ Transcription Kit (ASF3507, Lucigen) was used according to the manufacturer protocol with 4 μL PCR products per 20 μL reaction ...
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bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide containing the Illumina P7 adaptor sequence was ligated to the 3’ end of the single stranded LAM PCR fragments using Circligase (Epicentre/Illumina, #CL9025K). A P5 adaptor was added by PCR ...
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bioRxiv - Neuroscience 2020Quote: ... using Inpp5e WT forward primer 5’-GAGAAGCTGATAGATGGCTAGG and Inpp5e WT reverse primer 5’-AACCAGAAGACCTCATCAAACC and EconoTaq PCR according to manufactures specifications (Lucigen corporation, WI) (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). The genotyping primers were as described previously29,30,92
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). For embryonic genotyping during primary culture preparation ...
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bioRxiv - Genomics 2022Quote: Shot-gun library was generated at the McGill Genome Center using the NxSeq® AmpFREE Low DNA Library Kit Library Preparation Kit (Lucigen Corp., WI, USA) according to the manufacturer’s recommendations and the library was ran on a HiSeq X for 2×150 cycles.
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were produced using the Art-Seq kit (Epicentre/Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Total DNA was purified using Masterpure DNA Purification kit (Epicentre), and prepared for TnSeq by PCR amplification of transposon:genome junctions and adapter ligation following the protocol in [53] ...
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Elizabethkingia anophelis response to iron stress: physiologic, genomic, and transcriptomic analysesbioRxiv - Microbiology 2019Quote: ... The Ribo-Zero rRNA removal kit (Gram-negative bacteria, Epicentre) was used to remove the ribosomal RNA species (23S and 16S rRNA ...
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bioRxiv - Microbiology 2019Quote: ... NxSeq DNA sample prep kit 2 (Lucigen, Middleton, WI, USA) was used as per manufacturer’s directions with either NEXTFlex 48 barcodes (BioO ...
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bioRxiv - Microbiology 2020Quote: ... with the Ribo-Zero rRNA removal kit for Bacteria (Epicentre) according to standard protocol ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen), and treated with TURBO DNase (ThermoFischer ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA was depleted using Ribo-ZERO magnetic kit (Epicentre). The transcripts were fragmented and used as templates to generate strand-specific cDNA libraries by TruSeq Stranded Total RNA LT Sample Prep kit (Illumina) ...