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Citations for Lucigen :
251 - 287 of 287 citations for Hrms Rapid Pcb Screening Calibration Solution Cs0.05 Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The electroporated progenitors were subjected to adipogenic differentiation for 8 days and the genomic DNA was isolated using QuickExtract DNA Extraction Solution (Lucigen). PCR reactions were conducted with 50 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche ...
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bioRxiv - Microbiology 2023Quote: ... following an adapted version of the manufacturers’ protocol including an additional step of adding 0.5 µL of Ready-Lyse™ Lysozyme Solution (Lucigen). Next ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using the Promega Maxwell Tissue DNA Kit with the addition of Readylyse Lysozyme Solution (Epicentre) and Lysostaphin (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA of Pkd1-/-or Pkd2-/- single cell clonal mNPC lines were extracted using QuickExtractTM DNA Extraction Solution (Lucigen, # QE09050). PCR primers were designed to flank the sgRNA targeting sites of Pkd1 or Pkd2 genes (listed below) ...
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bioRxiv - Genomics 2023Quote: ... the media was removed and genomic DNA was extracted using 30 uL/well of QuickExtract DNA Extraction Solution (Lucigen #QE09050) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution (corresponding to 10 µg of total RNA) was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 minutes at a temperature of 25°C ...
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bioRxiv - Genetics 2021Quote: ... and four CS (line 403 subclones 1, S7, S8, and S9) iPSC lines was extracted using quick extract DNA extraction solution (Epicentre #QE09050), amplified by PCR ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed once with DPBS and genomic DNA (gDNA) was extracted with 50 µL/well of Quick-Extract solution (Lucigen, QE09050). Lysates were pipetted up and down thoroughly ...
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bioRxiv - Genomics 2022Quote: ... and diluted at 1:100 ratio into 250 ml LB supplemented with kanamycin (50 μg/mL) and 1x copy number induction solution (Lucigen, CCIS125). Payload DNA was isolated from E ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was spun down at 5,000 x g for 5 minutes and the pellet was resuspended in 100 µl of QuickExtract™ DNA Extraction Solution (Lucigen) and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre ...
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bioRxiv - Cancer Biology 2021Quote: ... One of the plates was used for quick genomic DNA isolation for each of the colonies using QuickExtract DNA extraction solution (Lucigen # QE09050). 5μl of this lysate was used to set up a quick genomic PCR screening for IL-1β knockout clones using checking primers ...
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were counted and 1e6 cells for each sample were collected and washed with PBS before RNA were extracted by QuickExtract RNA Extraction Solution (Lucigen, QER090150). SuperScript IV VILO Master Mix (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... A piece of embryonic tail was taken from each embryo and DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from 2.5×105 Neuro2A cells with 250 μL of QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA) or from 30 mg of mouse liver using a EZ-10 Spin Column Animal Genomic DNA Miniprep Kit (Bio Basic ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA for CRISPR/Cas9 genotyping was isolated from cell lines and organoids by using Quick Extract DNA Extraction Solution (Lucigen, #QE09050). For organoids ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was extracted after 48-72 hrs incubation using 50 µL Quick Extract™ DNA Extraction Solution (Lucigen, Middleton, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Clones with the best response to TMP were expanded and lysed for gDNA extraction and purification using the QuickExtract DNA extraction solution (Lucigen, Middleton, WI). Genomic ecDHFR was amplified (forward ...
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bioRxiv - Molecular Biology 2020Quote: ... The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.
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bioRxiv - Genomics 2022Quote: ... Genomic DNA from edited and unedited HSPC samples was harvested 48h post-editing using QuickExtract DNA Extraction Solution according to manufacturer’s recommendations (Lucigen Corp., Teddington, UK) and diluted to 4.55 ng/uL in IDTE pH 8.0 (IDT ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR screening of the colonies was performed using genotyping oligos listed in Supplementary Table 1 using Quick Extract DNA Extraction Solution (Lucigen Catalog Number: QE09050) according to manufacturer’s protocol in a PCR machine and DreamTaq Green Polymerase (Thermo Fisher Scientific Catalog Number ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: The genomic DNA of individual control or Dll-KO embryos (sorted manually based on injection marker or on reporter expression) were extracted with 20 μl of Quick Extract ™ DNA Extraction Solution (Lucigen, Cat# QE09050) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... samples were centrifuged and resuspended in TE buffer and incubated overnight at 37°C after addition of the Ready-Lyse lysozyme solution (Lucigen, Middleton, WI, USA). gDNA extraction (Lucigen ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA from cell lines growing on 96-well plates was extracted by 25 µl QuickExtract DNA extraction solution (Epicentre #QE09050, Madison, WI, USA), further diluted to 200 µl with H2O ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... filled with 1 ml LB supplemented with 25 μg/ml chloramphenicol and 2 µl/ml CopyControl fosmid autoinduction solution (Epicentre Biotechnologies, Cat. No. AIS107F). The plates were grown at 30 °C shaking at 700 RPM for 16 h covered by an AeraSeal gas-permeable sheet (EXCEL Scientific Cat ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...