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Citations for Lucigen :
251 - 275 of 275 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 15-20 μg of genomic DNA (gDNA) was digested with ∼95 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Genomics 2024Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was then incubated at 37 °C for 1 h with 20 U of RNase R (Lucigen, cat n°RNR07250), to remove all the undesired products (i.e linear RNA or concatemer product) ...
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bioRxiv - Neuroscience 2024Quote: ... we pooled all mice into the same tube containing a hypertonic lysis buffer (10mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal, 0.2 U/μl Lucigen NxGen Rnase inhibitor). Nuclei were extracted using a glass dounce homogenizer (DWK ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... HBV plasmid (positive control) and HBV DNA from inoculum was digested with plasmid-safe DNase (Epicentre, E3101K, Madison, WI). According to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... adding additional ATP and DNase every 24 h (30 units per day) according to the manufacturer’s protocol (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,). ecDNA-enriched samples were used as template for phi29 polymerase amplification reactions (150043 ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37 °C for 5 min.The successful linearization of ecDNA was verified by verifying its sensitivity to exonucleaseATP-dependent DNase (Lucigen). After treatment with exonuclease ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA pellets were washed once in 80% ethanol and pellets were air-dried and dissolved in 11uL of water, which was then circularized with the addition of 9uL of CircLigase I mix (1M betaine, 1x CircLigase Buffer (Lucigen), 2.5mM MnCl2 ...
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bioRxiv - Microbiology 2024Quote: ... and approximately 80 µg of RNA in 1 mL volume was digested with 5 U of RNase I (Lucigen Cat#E0067-10D1) for 45 min at 25°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Molecular Biology 2022Quote: ... Fig 5B-D and Fig EV5 were obtained as follows: Total RNA was extracted from cells using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Epicentre), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (ThermoFisher Scientific) ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... during which additional ATP and DNase was added every 24LJh (30 units per day) according to the manufacturer’s protocol (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K). The eccDNA samples were cleaned by phenol ...
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bioRxiv - Molecular Biology 2024Quote: ... DNase I-treated and poly(A)-selected RNA was subjected to treatment with Terminator 5ʹ-Phosphate-Dependent Exonuclease (Epicentre; TER51020) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... first-strand cDNAs were synthesized from 1 µg of DNase-treated RNA using the MMLV Reverse Transcriptase Synthesis Kit (Lucigen) protocol with the Oligo(dT)21 primer ...
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bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
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bioRxiv - Genetics 2021Quote: ... a restriction digest is performed to exclusively linearize the parental plasmid followed by an overnight digestion with Plasmid-Safe™ ATP-Dependent DNase (Lucigen). Prior to MIJ ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was discarded and approximately 0.2 mL of the sample was incubated with DNases (Promega and Epicentre, Madison, WI, USA), RNase A (Ambion ...
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bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Microbiology 2023Quote: ... 1mL of cell culture was collected and used for genome DNA extraction using Lucigen MasterPure Complete DNA & RNA with additional RNase step (Lucigen, Mandel Scientific, Guelph, ON, Canada) following the manufactures’ manual.