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Citations for Lucigen :
1 - 50 of 275 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Neuroscience 2022Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... For RNAse-free IP conditions TBS-0.05% NP-40 was supplemented with 1U/ul NxGen RNAse inhibitor (Lucigen). For nuclease treated samples ...
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bioRxiv - Microbiology 2020Quote: ... PCR-free library preparation (Lucigen) and NextSeq 500 sequencing (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (ii) Shotgun PCR-free library preparation kit (Lucigen) or (iii ...
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bioRxiv - Genomics 2024Quote: ... using the Shotgun PCR Free Library Kit (Lucigen) and the NovaSeq™ 6000 Sequencing device (Illumina® ...
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bioRxiv - Microbiology 2023Quote: ... Free nucleic acids were digested using OmniCleave Endonuclease (Lucigen, USA) for 30 minutes ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Microbiology 2020Quote: ... Free mRNA and rRNA ends were then ligated into circular forms with a circRNA ligase (Lucigen); it favors ligation events between proximal RNA ends11 and it has limited activity at high temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 20 U RNase R and 10× RNase R buffer (Epicentre) was added according to manufacturer’s protocols and was heated to 37 for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase R (Lucigen) digestion was performed to degrade linear RNA ...
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bioRxiv - Genomics 2023Quote: ... RNase inhibitor (Lucigen) was added at 0.4 U/µl throughout the incubation until the rolling cycle amplification (RCA ...
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bioRxiv - Genetics 2022Quote: ... RNase Inhibitor (Lucigen) and NxGen T7 RNA polymerase (Lucigen ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 1U of RNase H (Hybridase Thermostable RNase H, Epicentre), purified using RNA Cleanup XP beads (Agencourt) ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 1U of RNase H (Hybridase Thermostable RNase H, Epicentre), purified using RNA Cleanup XP beads (Agencourt) ...
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bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...
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bioRxiv - Plant Biology 2021Quote: ... a PCR-free library was prepared with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified genomic DNA from each individual was used to prepare paired-end libraries (Lucigen Shotgun library preparation, PCR free) and sequenced on an Illumina HiSeq X PE 150 bp platform to 10-20X coverage at the Centre d’ Expertise et de Services ...
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bioRxiv - Physiology 2024Quote: ... RNase inhibitor (30281, Lucigen), Maxima H-RTase (EP0751 ...
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bioRxiv - Neuroscience 2022Quote: RNAse R (Lucigen, #RNR07250) treatment was performed on 1 μg total RNA for 10 minutes at 37°C using 5U of enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase A (0.1mg; Lucigen) was added ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μl RNase H buffer and 2 μl hybridase thermostable RNase H (Lucigen) preheated to 45° were added ...
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bioRxiv - Microbiology 2022Quote: ... DNA-Seq library preparation and sequencing were performed by the Genome Quebec Company (Genome Quebec Innovation Center, McGill University, Montreal, Canada) using the Shotgun PCR-free library preparation kit (Lucigen) and the NovaSeq™ 6000 Sequencing system (Illumina® ...
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bioRxiv - Microbiology 2023Quote: ... and purified DNA-free RNA samples were subjected to ribosomal depletion with Ribo-Zero™ Magnetic Kits (Epicentre®, Singapore), all according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... DNase I (Epicentre) digestion was performed according to the manufacture’s instruction to minimize contamination with plasmid DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 18.75 U RNase R (Lucigen), 4.375 U RNase T (NEB) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Step 2: Riboshredder RNase (Epicentre) was used in place of RNase A ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNase inhibitor (Epicentre), 1X NEB buffer 3.1 (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNase inhibitor (Epicentre), and nuclease-free water (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase H (10U; Lucigen, H39500) was added and the mixture incubated at 45°C for 30 min in 20 μl containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Immunology 2024Quote: ... for which RNase H (Epicentre) treatment was used ...
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bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM KCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.25 units RNase I (Epicentre) was added to 5ul of sample in a total volume of 50 ul in IP buffer supplemented with 0.1% Igepal ...
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bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM LiCl ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL RNAse A (Lucigen) was added to the cells and incubated for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... RNase-H master mix was prepared for each depletion (3µl Hybridase Thermostable RNase H; Epicentre), 0.5µl 1M Tris-HCl pH=7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Total RNAs (450 ng) were treated with DNase (Baseline-ZERO DNase, Epicentre) and ribosomal RNA was removed using the Illumina Ribo-Zero Magnetic Kit (Bacteria) ...
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bioRxiv - Microbiology 2024Quote: ... Baseline ZERO DNAse (Lucigen), Benzonase (Sigma-aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 4 μl Actinomycin D (500ng/μl ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 2 μl Maxima H- RTase (EP0751 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen) and 31 μl water ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 U/ml Rnase Inhibitor (Lucigen), 2.5 μM Template Switch Oligo ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl RNase R mixture (Epicentre) was added to the sample before incubation at 37°C for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl of RNase inhibitor (Lucigen) and 450 μl of IP buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 U NxGen RNase Inhibitor (Lucigen), and 40 U Superscript IV RT (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2U/uL RNAse inhibitor (Lucigen). The tissue was then triturated 15 times through a fire-polished Pasteur pipette and then passed through a 40 uM filter to remove cell debris and large chunks ...