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Citations for Lucigen :
251 - 300 of 352 citations for 1 2 Bis Pentabromophenyl Ethane Unlabeled 25 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... and 1 μl of CircLigase I (Epicentre, Madison, WI). Ligation reactions were incubated at 60C for 2 h and the ligase was heat inactivated by incubating at 80C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was prepared from 3 mL of turbid liquid culture with the MasterPureTM Gram Positive DNA Purification Kit (Lucigen). DNA was quantified by using a NanodropTM device (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 50 ml of culture was used for DNA extraction using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, WI, USA).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was discarded and approximately 0.2 mL of the sample was incubated with DNases (Promega and Epicentre, Madison, WI, USA), RNase A (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2020Quote: ... in combination with 1 µl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... in combination with 1 μl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Molecular Biology 2020Quote: ... The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.
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bioRxiv - Plant Biology 2020Quote: ... A mixture consisting of 1 μl 10x Circligase reaction buffer (Epicentre), 0.5 μl 1 mM ATP ...
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bioRxiv - Genomics 2021Quote: ... 1.5 μl ATP and 1 μl FastLink DNA ligase (Lucigen Corporation), followed by heat inactivation for 15 min at 70°C ...
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bioRxiv - Genomics 2022Quote: ... including a 1:4 ratio of biotin-16-UTP (BU6105H, Lucigen) to UTP at 5 mM ...
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bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Synthetic Biology 2021Quote: ... a highly competent derivative of DH10β was obtained from Lucigen (60107-1)53 ...
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bioRxiv - Neuroscience 2022Quote: ... together with 1 1l genomic DNA in QuickExtract DNA Extraction Solution (Lucigen). After droplet generation with the QX200 Droplet generator (Biorad) ...
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bioRxiv - Biochemistry 2022Quote: ... The electroporated bacteria were then cultured in recovery medium (Lucigen 80026-1) for 1 hour ...
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bioRxiv - Genetics 2019Quote: ... 1 μg of RNA was treated with 20 units of RNAseR (Epicentre) in a 25μl reaction at 37°C for 30 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were grown in E.Cloni 10G Chemically Competent Cells (Lucigen, 60107-1) and were verified by Sanger sequencing (Eton biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 2.5 U of CircLigase II ssDNA Ligase (Lucigen), and incubating the resultant mixtures at 40°C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... Ruptured cells were treated with 1 U/μl OmniCleave endonuclease (Lucigen, OC7850K) for 5 min at 37°C to release the DNA-bound protein pool ...
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bioRxiv - Genetics 2023Quote: ... Final assembled products were delivered to Endura Electrocompetent Cells (Lucigen, 60242-1) via electrotransformation (Bio-Rad Gene Pulser II) ...
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bioRxiv - Genomics 2021Quote: ... and used to electroporate 1-4 tubes of Endura DUO electrocompetent cells (Lucigen) at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) containing 50 U/μL Ready-Lyse Lysozyme (Lucigen #R1804M) and protease inhibitors ...
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bioRxiv - Genetics 2022Quote: Transposon mutagenesis was performed using the EZ-Tn5
1> transposon kit (Epicentre) and EK54/MA56/LP372 Tn5 transposase ... -
bioRxiv - Microbiology 2021Quote: ... the remainder of samples were lysed by adding 1 ul of ReadyLyse (EpiCentre) and incubating at room temperature for 12 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to end-repair with T4 PNK (Lucigen, 37C for 1 hr). End-repaired RNA was cleaned up with the RNA Clean and Concentrator kit (Zymo) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli genomic DNA in 15 μL of 1× Ampligase DNA Ligase Buffer (Epicentre) containing 250 ng of unshared E ...
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bioRxiv - Genetics 2022Quote: ... The ligated product was then electroporated into Enduratm electro-competent cells (Lucigen #60242-1) in 2 separate reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10G isa highly competent DH10B derivative [54] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1.5 μL of 5 U μL-1 Ampligase Thermostable DNA Ligase (Lucigen, USA). Water was added to a total volume of 25 μL ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
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bioRxiv - Biochemistry 2021Quote: ... The dsDNA was purified from a 1% agarose gel and electroporated into E.coli SS320 (Lucigen) electrocompetent cells pre-infected with M13KO7 helper-phage (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... The sample suspension was digested with 1 μL Ready-Lyse lysozyme (1000 U/ μL, Epicentre), and 3 μL proteinase K (20 mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Single cells were FACS sorted into 1 ul each of QuickExtract DNA Extraction Buffer (Lucigen). DNA was extracted by incubating cells for 10 minutes at 65°C followed by 5 minutes at 98°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Collagenase and EDTA solutions were prepared with RNAse inhibitor added (1:100 ratio, Lucigen, 30281). Isolation buffer is composed of 70 mM NaCl ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli 10G is a highly competent DH10B derivative [39] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Bioengineering 2023Quote: ... each reaction mixtures were transformed into Endura™ electro-competent cells (Lucigen, CAT. 60242-1) and transformed bacteria were selected on LB + Ampicillin plates ...
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bioRxiv - Microbiology 2020Quote: Transposon mutagenesis was performed using the EZ-Tn5™
1>Tnp Transposome™ Kit (Epicentre), according to manufacturer’s specifications ... -
bioRxiv - Plant Biology 2021Quote: ... The RNA (1-3 μg) was then treated with 5 units of RNase R (Lucigen. RNR07250) for one hour at 37°C ...