Labshake search
Citations for Lucigen :
201 - 250 of 352 citations for 1 2 Bis Pentabromophenyl Ethane Unlabeled 25 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 1× CircLigase II buffer (Lucigen, CL9021K), and 2.5 mM MnCl2 was added to 16 µL of cDNA and incubated at 60°C for 100 min followed by 80°C for 10 min ...
-
bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
-
bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
-
bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
-
bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
-
Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
-
bioRxiv - Evolutionary Biology 2023Quote: A transposon mutant library for the WT strain was generated by electroporation using the EZ-Tn5
2> Tnp transposome kit (Lucigen Inc.). The library consisted of approximately 40,000 insertion mutants ... -
bioRxiv - Genetics 2022Quote: ... Supernatants were discarded and the remaining 0.2 mL were digested with DNases (Promega and Epicentre, USA), RNase A (Ambion ...
-
bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
-
bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
-
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.
-
bioRxiv - Developmental Biology 2023Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
-
bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
2> Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Developmental Biology 2024Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 u/µl NxGen RNase inhibitor (Lucigen) and 5% (v/v ...
-
bioRxiv - Systems Biology 2022Quote: ... coli cells (Lucigen cat. no. 60242-1) using prechilled 1 mm electroporation cuvettes (BioRad cat ...
-
bioRxiv - Bioengineering 2019Quote: ... and 1 μL 10X ampligase buffer (Lucigen). Circularization was achieved under the following conditions ...
-
bioRxiv - Microbiology 2019Quote: ... 1 μl of RiboGuard RNase Inhibitor (Epicentre) was added to the eluted RNA to protect against ribonucleases ...
-
bioRxiv - Genomics 2019Quote: ... 1 μL ScriptSeq Index PCR Primer (Epicentre), 1 μL Reverse PCR Primer (Epicentre) ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 1:200 diluted endonuclease (Lucigen: OC7850K) was immediately added to the pulverized tissue at a constant ratio of 15μL/mg of tissue ...
-
bioRxiv - Genomics 2021Quote: ... 10uL 10x OmniAmp buffer (Lucigen, F883707-1), 8uL dNTP (NEB ...
-
bioRxiv - Genomics 2021Quote: ... 1 U/μL Rnase Inhibitor (Lucigen, #F83923), 2.5 μM Template-Switching Oligo primer ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 x Ampligase® Reaction Buffer (Lucigen), 10 U Ampligase (Lucigen) ...
-
bioRxiv - Genomics 2022Quote: ... 1 U/μL RNase Inhibitor (NxGen, Lucigen), 1 U/μL reverse transcriptase (ThermoFisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... in 1× RNase R buffer (#RNR07250; Epicentre) in a 10 µl reaction volume at 37 °C for 10 min followed by heat inactivation at 95 °C for 3 min ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...
-
bioRxiv - Molecular Biology 2022Quote: RNA was extracted from 1.5 mL of logarithmically growing cells (OD600 ~0.6-0.8) using the Masterpure Yeast RNA Purification kit (Epicentre). Residual genomic DNA was removed using the Turbo DNA-free kit (Ambion) ...
-
bioRxiv - Genomics 2022Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30°C with shaking at 220 RPM ...
-
bioRxiv - Genomics 2023Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30 °C with shaking at 220 RPM ...
-
bioRxiv - Biochemistry 2020Quote: ... pNT-15 was constructed using NEB HiFi DNA Assembly with a gBlock® from IDT (Coralville, IA) and cloning vector pSMART-HCKan (Lucigen, #40704-2). All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
-
bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
-
bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
-
bioRxiv - Systems Biology 2021Quote: ... We then added 3.8 mL of 1x BMg/THS buffer and 0.8 μL of ReadyLyse lysozyme solution (Lucigen), 40 μL of 10 mg/mL RNase A ...
-
bioRxiv - Cell Biology 2023Quote: Cells plated on glass coverslips were incubated with 5 µg/mL Brefeldin A (BFA) (Epicentre Biotechnologies, Madison, WI) for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: ... ratio overnight at 16 °C followed by purification and then electroporation into phage-display competent TG-1 cells (#60502-1, Lucigen). The library was plated onto 2xYT agar plates containing 100 µg/mL carbenicillin and 2% glucose at 37 °C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... GU332722.1) or Asia II 1 (GenBank accession no. GU332721.1) was synthesized using the AmpliScribe T7- Flash Transcription Kit (Epicentre, ASF3507), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
-
bioRxiv - Microbiology 2020Quote: Samples were analyzed directly or mixed 1:1 with one of the following buffers: Quick Extract DNA Extraction Solution (Lucigen), Virotype Tissue Lysis Reagent (INDICAL BIOSCIENCE GmbH ...
-
bioRxiv - Molecular Biology 2020Quote: ... Comparable results to commercial RNA extraction kits have been obtained using a 5-min direct detection preparation method of nasopharyngeal samples following 1:1 dilution with the Quick Extract DNA extraction Solution (Lucigen) (6) ...
-
bioRxiv - Genomics 2022Quote: ... 4 μl of T5-Tn5 transposome and 4 μl of T7-Tn5 transposome were mixed to form 8 μl of assembled Tn5 transposome before it was mixed with 1 μl cDNA (containing ~500 pg cDNA) and 1 μl reaction buffer (TNP92110, Lucigen). The reaction mixture was incubated at 37°C for 1 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology) ...
-
bioRxiv - Genomics 2020Quote: ... 1 ul Hybridase Thermostable RNase H (Lucigen, H39500) and 1 ul 10 X digestion buffer (500 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... using Endura™ ElectroCompetent Cells (Lucigen 60242-1). Resulting pooled DNA libraries were used to produce lentiviral particles and infect primary cortical neurons.
-
bioRxiv - Plant Biology 2021Quote: ... coli C41 (DE3) cells (Lucigen, Cat# 60442-1) and purified using Ni-NTA agarose (Qiagen ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology ...
-
bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
-
bioRxiv - Cancer Biology 2024Quote: ... To obtain ribosome footprints 0.12 ml of total extracts containing 300 µg of total RNA were treated with RNAse I (Epicentre) (25U/1 mg of total RNA) ...
-
bioRxiv - Biochemistry 2019Quote: ... For RNase III tests 1 unit of enzyme (Epicentre) was added 10 min after addition of RNases I and H ...