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Citations for Lucigen :
201 - 250 of 304 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre, Cat # QE09050) to the cells ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Bioengineering 2024Quote: ... DNA extraction from various hiPSC clones was carried out using the QuickExtract DNA Extract Solution (Epicentre), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from each CFU recovered using the QuickExtract DNA extraction solution (Epicentre, # QE09050) and each colony was screened for the presence of tbpA and lbpA genes ...
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bioRxiv - Molecular Biology 2024Quote: ... a fraction of the cells was used for genomic DNA extraction using QuickExtract DNA Solution (Lucigen). PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA was extracted from individual samples using 40 µL QuickExtract DNA extraction solution (Lucigen QE0905T). Embryos were incubated for 15 min at 25 °C ...
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bioRxiv - Genetics 2024Quote: ... they were further expanded and gDNA was harvested using Quick Extract DNA Extract Solution (Lucigen, QE9050). Excision and inversion clones were genotyped via ddPCR and Sanger sequencing (Table S66).
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA templates were prepared by lysing cells in QuickExtract DNA extraction solution (Lucigen, Cat No. QE0905T). Target regions were amplified by using specific PCR primers (ETV2_F ...
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bioRxiv - Cell Biology 2022Quote: ... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
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bioRxiv - Genomics 2022Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30°C with shaking at 220 RPM ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Genomics 2023Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30 °C with shaking at 220 RPM ...
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bioRxiv - Developmental Biology 2022Quote: Tissue samples (ear and tail clips) from resulting animals were lysed using QuickExtract DNA Extraction Solution (Epicentre) followed by PCR at the specific genomic location ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from individual female adult flies from stocks of interest using QuickExtract solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: One swab each was stored in 350 μl Tissue and Cell lysis solution (Lucigen, #MTC096H, Middleton, WI) lysis buffer with 100 μl glass beads (0.1 diameter ...
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bioRxiv - Developmental Biology 2022Quote: ... yolk sacs were dissected and a small fraction was placed in QuickExtract DNA Extraction Solution (Lucigen, QE09050) and processed according to manufacturer’s protocols.
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bioRxiv - Cell Biology 2022Quote: ... the individual clones were expanded and the DNA was isolated using the QuickExtract DNA Extraction Solution (Lucigen). A successful homozygous genomic modification was confirmed by PCR (data not shown ...
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bioRxiv - Biophysics 2023Quote: ... Genomic DNA was extracted 3 days post-transfection using QuickExtract DNA Extraction Solution 1.0 (Lucigen Corporation QE09050). To test the cutting efficiency ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from these clones using the QuickExtract DNA extraction solution (Catalog No. QE09050, Lucigen) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... gDNA isolation was performed from a portion of the clones via QuickExtract DNA Extraction Solution 1.0 (Epicentre). Genotyping primers were created by designing flanks around the mutation site ...
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bioRxiv - Molecular Biology 2024Quote: ... for sorting and then genomic DNA was extracted using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre), adhering to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... an aliquot of cells was used to extract the DNA by QuickExtractTM DNA Extraction Solution (Lucigen, QE09050). To assess the genomic editing efficiency ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... the genomic DNA derived from transfected single cell colonies was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for Sanger sequencing and the genomic DNA with biallelic editing was further subjected to whole-genome sequencing ...
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bioRxiv - Systems Biology 2021Quote: ... We then added 3.8 mL of 1x BMg/THS buffer and 0.8 μL of ReadyLyse lysozyme solution (Lucigen), 40 μL of 10 mg/mL RNase A ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from 10,000 – 50,000 target cells by resuspending them in a QuickExtract DNA Extraction Solution (LGN-QE09050, Lucigen). PCR amplification of the targeted junction from the wild-type and tagged cells was performed using primers designed to anneal outside of the homology arms of the specific gene ...
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bioRxiv - Microbiology 2020Quote: ... The samples were chilled on ice for 5 minutes and 175 μl of MPC protein precipitation solution (Lucigen, USA) was added to 300 μl of the lysed sample and vortexed vigorously for 10 seconds ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated for 10 min at 37°C in the presence of Ready-Lyse lysozyme solution (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA of the two parents and the F2 progeny was extracted with the QuickExtract DNA Extraction Solution (Lucigen, QE0905T) according to manufacturer’s protocol with modifications ...
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bioRxiv - Microbiology 2023Quote: ... The culture was diluted again to 400 mL of LB30 medium + 12.5 μg/mL of chloramphenicol + 1x Arabinose induction solution (Lucigen) for overnight ...
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bioRxiv - Genetics 2023Quote: ... and plasmid replication was induced by diluting 1:10 in LB + 12.5 µl/ml chloramphenicol and 1X CopyControl Induction Solution (Lucigen). The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
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bioRxiv - Immunology 2022Quote: Genotyping was performed by extracting genomic DNA from fin clips or larvae using the QuickExtract DNA extraction solution (Epicentre) and amplifying loci using EMBL in-house Phusion polymerase ...
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bioRxiv - Genetics 2023Quote: ... cells were washed once with 500 µl PBS and genomic DNA was harvested using QuickExtract DNA extraction solution (Epicentre) following manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... Supernatant were carefully discarded and the isolated hair cells were lysed by 5μl QuickExtract™ DNA Extraction Solution (Lucigen) and incubated in 65°C for 6 min then 98°C 3 min ...
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bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... During this process individual embryos were collected at 6 and 24 hpf in QuickExtract™ DNA Extraction Solution (Lucigen) for Indel mutations genotyping using MiSeq sequencing.
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bioRxiv - Neuroscience 2021Quote: ... 6µl 500X CopyControl Fosmid Autoinduction Solution was also added to induce the fosmid to high copy according to the CopyControl Fosmid Library Production Kits (EPICENTRE). The fosmid was then prepared using the standard Qiagen miniprep kit.
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bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
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bioRxiv - Genetics 2021Quote: ... DNA extraction from 2 x 2 x 2mm fin-tissue clips was performed in a 96-well PCR plate using 100μl of QuickExtract solution (Lucigen, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Cell debris was then pelleted and used for gDNA extraction with 10-20 µl QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from bulk cells transfected with CRISPR/Cas9 plasmids using the epicentre QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...