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Citations for Lucigen :
151 - 200 of 304 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Biochemistry 2021Quote: ... from which the genomic DNA was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for subsequent sequencing analysis.
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cell lines using QuickExtract DNA Extraction Solution (Epicentre, #QE09050). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA of expanded clones was isolated using QuickExtract DNA extraction solution (Lucigen, QE0905T). PCR was performed by amplifying a 241bp area surrounding the mutation site ...
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bioRxiv - Neuroscience 2021Quote: ... the genomic DNA was extracted using the QuickExtract DNA Extraction solution (Epicentre, Madison, USA), according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2020Quote: ... which were washed with PBS and resuspended in QuickExtract DNA Extraction Solution (Epicentre, QE09050). Cells were incubated at 68 °C for 15 min and 95 °C for 8 min and the integrated synthetic VL-Ck-2A-VH antibody region was PCR-amplified with flanking primers 5’-CATGTGCCTTTTCAGTGCTTTCTC-3’ and 5’-CTAGATGCCTTTCTCCCTTGACTC-3’ that were specific for the 5’ and 3’ homology arms ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA of grown clones was harvested using QuickExtract™ DNA Extraction Solution (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA (gDNA) was extracted by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was isolated from ear biopsy samples utilizing QuickExtractTM DNA Extraction Solution (Epicentre, QE09050). The tissue was incubated in 20µl QuickExtractTM at 65°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (QE0905T; Lucigen), and PCR products containing the site of Cas9 targeting site were generated using the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from puromycin-resistant clones using QuickExtract DNA extraction solution (Lucigen), PCR amplified and tested on agarose gel for accurate deletion.
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA of 3T3 cells was isolated with QuickExtractTM DNA Extraction Solution 1.0 (QE09050, Lucigen). Presence of the homozygous deletion was confirmed by PCR using specific primers ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from transfected cells using the QuickExtractTM DNA Extraction Solution (Lucigen) and mutations were detected by the SURVEYOR assay (Integrated DNA technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (Lucigen QE0905T), and PCR products containing the site of Cas9 cleavage site were generated using the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA from single clones was extracted using QuickExtract DNA extraction solution (Lucigen; QE0905T) according to the manufacturer’s suggestion ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by addition of 50 μl QuickExtract DNA solution (Epicentre, QE09050) to the cells ...
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bioRxiv - Microbiology 2024Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Molecular Biology 2021Quote: ... after which genomic DNA was isolated using QuickExtractTM DNA Extraction Solution (Epicentre, Madison, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was harvested using QuickExtract solution (screening of CRISPR/Cas9 clones; Epicentre EPI-QE0905T) or NucleoSpin Tissue kit (routine genotyping ...
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bioRxiv - Microbiology 2021Quote: ... To extract DNA 10 ul of culture was added to 500 ul QuickExtract Solution (Epicentre) and treated 65 °C for 6 min followed by 98 °C for 2 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... and lysed in containing 200 μl of QuickExtract™ DNA Extraction Solution (Lucigen Cat. # QE09050) per well of 48-well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... The genomic DNA from individual cell clones was extracted using QuickExtract DNA Extraction Solution (Lucigen) and screened by PCR for the deletion amplicon using the DNA primers listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... and DNA was extracted using the QuickExtract DNA Extraction Solution (Epicentre, Madison, WI, USA, #QE09050) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Collagenase and EDTA solutions were prepared with RNAse inhibitor added (1:100 ratio, Lucigen, 30281). Isolation buffer is composed of 70 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and positional insertion of mGFP/mScarlet-I cassette ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). Primers specific to the blasticidin resistance cassette and IRAK1/IRAK4 gene loci were used to verify the insertion (see Table S5 and S6 for IRAK4 and IRAK1 respectively) ...
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bioRxiv - Immunology 2020Quote: ... DNA was isolated from the clones using the QuickExtract DNA Extraction Solution (Lucigen, Middleton, USA). Upon amplification and sequencing (Eurofins Genomics ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen, Middelton, WI, USA). 50 ng of genomic DNA were used to amplify the region that spans the cutting site of each gRNA using KAPA2G Fast ReadyMix (Kapa Biosystem ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... The CRISPR/Cas9-edited mutants were validated by DNA extraction using QuickExtract solution (Lucigen, QER090150) followed by sanger sequencing prior cell line expansion28,38 ...
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bioRxiv - Biochemistry 2022Quote: ... the media was removed by aspiration and 100μl of Quick Extraction solution (Epicentre, Madison, WI) was added to lyse the cells (65°C for 20 min and then 95°C for 20 min) ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and correct insertion of mGFP/mScarlet-i cassette ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from single cell clones using QuickExtract DNA Extraction Solution 1.0 (Lucigen) and the region of interest was amplified by two consecutive PCRs ...
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bioRxiv - Synthetic Biology 2024Quote: ... and cells were resuspended in 50 µl of QuickExtract DNA Extraction Solution (Lucigen, Catalog #QE09050). Genomic DNA was extracted by treating the cells to the following protocol ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from iPSCs and NPCs using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: Genomic DNA was isolated from candidate cell lines using the QuickExtract DNA Extraction Solution (Lucigen, #QE09050) essentially as described ...
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bioRxiv - Synthetic Biology 2020Quote: ... and genomic DNA was extracted using 50 μL of Quick Extract DNA Extract Solution (Lucigen, QE09050) per well according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets were collected 72-96 hours after electroporation and resuspended in QuickExtract solution (Lucigen, QE09050) and subjected to genomic DNA extraction while incubating for 10 min at 65 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans Fosmid Library (Source BioScience) and were amplified in bacteria using CopyControl Induction Solution (Lucigen, #CCIS125) and purified using a DNA midi prep kit (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...
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bioRxiv - Synthetic Biology 2022Quote: ... with QS solution (1.5 M NaCl, 100 mM MOPS, 15% v/v isopropanol) and PlasmidSafe (Epicentre) were used in lieu of the Qiagen Large Construct Kit and Qiagen exonuclease ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre Cat. # QE09050) to the cells ...