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Citations for Lucigen :
101 - 150 of 338 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... BACs were isolated from 50ml bacterial cultures using the BACMAX Bac extraction kit (Epicentre). Isolated BACs were sonicated (x10 cycles of 15 seconds ‘on’ 45 seconds ‘off’ at constant intensity with power set to ‘3’ ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10G isa highly competent DH10B derivative [54] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from candidate mutants with the MasterPure DNA purification kit (Epicentre) and PCR followed by gel electrophoresis confirmed correct integration of the NAT dominant resistance marker ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µl from ligation reaction was added onto 25 µl of electrocompetent cells (Lucigen) and cells were transformed by using Electroporator (Bio-Rad MicroPulser ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was prepared using the MasterPure™ Yeast DNA purification kit from Lucigen. To determine the MAT ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (Lucigen QE0905T), and PCR products containing the site of Cas9 cleavage site were generated using the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA from single clones was extracted using QuickExtract DNA extraction solution (Lucigen; QE0905T) according to the manufacturer’s suggestion ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Ampligase Thermostable DNA Ligase for LCR reaction was obtained from Lucigen (Middleton, USA).
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bioRxiv - Microbiology 2022Quote: ... DNA extraction from pellets was done using MasterPure™ DNA purification kit (Epicentre, US) according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from puromycin-resistant clones using QuickExtract DNA extraction solution (Lucigen), PCR amplified and tested on agarose gel for accurate deletion.
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bioRxiv - Neuroscience 2024Quote: ... DNA was isolated from ear biopsy samples utilizing QuickExtractTM DNA Extraction Solution (Epicentre, QE09050). The tissue was incubated in 20µl QuickExtractTM at 65°C for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from flash frozen pellets using 30 μl of QuickExtract (Lucigen # QE09050) and samples were heated to 60 °C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: The Escherichia coli strains TG1 and AXE688 (21, 22, 23) were purchased from Lucigen Corporation (Middleton ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from transfected cells using the QuickExtractTM DNA Extraction Solution (Lucigen) and mutations were detected by the SURVEYOR assay (Integrated DNA technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (QE0905T; Lucigen), and PCR products containing the site of Cas9 targeting site were generated using the following primers ...
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bioRxiv - Microbiology 2024Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Systems Biology 2024Quote: ... cDNA libraries were constructed by using the TotalScript RNA-Seq kit from Epicentre (discontinued), a transposon-based method of tagmentation (simultaneous tagging and fragmentation ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from pelleted cells using a MasterPure Yeast RNA extraction kit (Lucigen, MPY03100), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The dsDNA was purified from a 1% agarose gel and electroporated into E.coli SS320 (Lucigen) electrocompetent cells pre-infected with M13KO7 helper-phage (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... and RNA was extracted from each replicate using the MasterPure Yeast RNA Purification Kit (Epicentre). Integrity ...
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bioRxiv - Cancer Biology 2020Quote: ... The genomic DNA from individual cell clones was extracted using QuickExtract DNA Extraction Solution (Lucigen) and screened by PCR for the deletion amplicon using the DNA primers listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification and precipitation of RNA were performed using the MasterPure RNA Purification Kit from Epicentre by adding 200 μl of 2X T and C Lysis Solution and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Germany). MasterPure DNA purification kit (Cat. No. MCD85201) was obtained from Epicentre (Middleton, WI, USA). GoTaq Green Master Mix (Cat ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from at least 104 harvested cells using the QuickExtract protocol (Lucigen). The target locus was then amplified by PCR with forward and reverse primers where at least one primer annealed outside the integration site (Supplementary Table 1) ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 104 to 105 T cells was was extracted using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using primers F2(RB198)* and R2(RB199)* primers (Supplemental Table 1) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from co-cultures with the MasterPure complete DNA purification kit (Lucigen). The proportion of G ...
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bioRxiv - Microbiology 2020Quote: ... end-repaired DNA from PoV-01B (1-2 Kb) were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and positional insertion of mGFP/mScarlet-I cassette ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). Primers specific to the blasticidin resistance cassette and IRAK1/IRAK4 gene loci were used to verify the insertion (see Table S5 and S6 for IRAK4 and IRAK1 respectively) ...
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bioRxiv - Immunology 2020Quote: ... DNA was isolated from the clones using the QuickExtract DNA Extraction Solution (Lucigen, Middleton, USA). Upon amplification and sequencing (Eurofins Genomics ...
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Intra-species transcriptional profiling reveals key regulators of Candida albicans pathogenic traitsbioRxiv - Genomics 2021Quote: ... RNA was harvested from cells using a Masterpure Yeast RNA Purification kit (Epicentre, Madison, WI) and treated with DNaseI (Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from each of the four cell pools with 100 µL QuickExtract (Lucigen) per 1 million cells following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from cell pellets using the MasterPure yeast DNA purification kit (Epicentre). Melanin was removed from genomic DNA using the OneStepTM PCR Inhibitor Removal kit (Zymo Research) ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was isolated from sporulation cultures using MasterPure Yeast DNA Purification kit (Lucigen MPY80200). Processing of genomic DNA samples and Southern blot analysis used to visualize crossovers and noncrossovers at the HIS4LEU2 hotspot were done as in [165] ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA was depleted from 10μg total RNA using the Ribo-Zero Magnetic Kit (Epicentre). Ribo-depleted RNA was fragmented ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and correct insertion of mGFP/mScarlet-i cassette ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from single cell clones using QuickExtract DNA Extraction Solution 1.0 (Lucigen) and the region of interest was amplified by two consecutive PCRs ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from iPSCs and NPCs using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
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bioRxiv - Synthetic Biology 2020Quote: Golden gate assembly reactions were transformed into chemically competent Escherichia coli prepared from strain TG1 (Lucigen). Transformed cells were selected on Lysogeny Broth containing the antibiotics chloramphenicol ...
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bioRxiv - Microbiology 2021Quote: Ribosomal RNA was removed from strain B50 transcriptomes by using the Ribo-Zero Magnetic Kit (EPICENTRE Biotechnologies ...
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bioRxiv - Biophysics 2020Quote: Genomic DNA was isolated from candidate cell lines using the QuickExtract DNA Extraction Solution (Lucigen, #QE09050) essentially as described ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from bacterial cell pellets using MasterPure™ complete DNA purification kit (Lucigen). Oxford Nanopore Minion Sequencing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was isolated from PHH using a MasterPure Complete DNA Purification kit (Epicentre, Madison, WI; MC85200) according to the manufacturer’s instructions ...