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Citations for Lucigen :
951 - 1000 of 1243 citations for DNA Damage 8 OHdG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted using a MasterPure™ Yeast RNA Purification Kit (Lucigen MPY03100), the RNA concentration was determined on a Nanodrop 2000C (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the ScriptSeq™ Complete Kit (Epicentre, Madison, WI, USA) according to the manu-facturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted using the Ribo-Zero™ Kit (Epicentre, Madison, WI, USA). The viral dsRNA of these strains was extracted and prepared as previously described [22] ...
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions and stored in RNase-free Tris-EDTA buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Bioengineering 2023Quote: ... We synthesized mRNA using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, catalog #ASF-3507) as described previously.(79 ...
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bioRxiv - Developmental Biology 2024Quote: ... The dsRNA was synthesized using the AmpliScribe T7 High Yield Transcription Kit (Lucigen, USA). Primer sequences for the PCR amplification are provided in Supplementary Table S3.
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre, Madison WI) and RNA purity and concentration were confirmed using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Biochemistry 2019Quote: ... For RNase III tests 1 unit of enzyme (Epicentre) was added 10 min after addition of RNases I and H ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Electroporations were recovered in 1 mL recovery media (Lucigen) for 1 hour and subsequently grown overnight in LB + selection.
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bioRxiv - Biochemistry 2019Quote: ... and 1 μl of CircLigase I (Epicentre, Madison, WI). Ligation reactions were incubated at 60C for 2 h and the ligase was heat inactivated by incubating at 80C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μL of Endura electrocompetent bacteria (Lucigen 60242-1) were transformed with 150 ng of library DNA using a 0.1 cm Gene Pulser electroporation cuvette (Bio-Rad 1652083 ...
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bioRxiv - Systems Biology 2020Quote: ... ribosomal RNA (rRNA) was removed using the Ribo-Zero Magnetic Kit for bacteria (Epicentre, IL). Metatranscriptome library preparation was performed using the Ion Total RNA-Seq Kit v2 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... DNAse-treated RNA (5 µg) was mRNA enriched using a Ribo-Zero Magnetic Kit (Epicentre). After Illumina sequencing the reads were mapped to C ...
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bioRxiv - Molecular Biology 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Genomics 2020Quote: ... and rRNA depletion was performed using Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina).
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bioRxiv - Genomics 2019Quote: ... The resulting template was transcribed using the Lucigen AmpliScribe T7-flash Transcription Kit from Epicentre/Illumina (cat ...
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bioRxiv - Plant Biology 2019Quote: ... RNA-seq library construction was carried out with the ScriptSeq™ v2 kit (Epicentre SSV21124). Final libraries were amplified with 13 PCR cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from pelleted cells using a MasterPure Yeast RNA extraction kit (Lucigen, MPY03100), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... PP contamination was removed using the Plasmid Safe ATP-dependent DNase kit (Epicentre, WI, USA), and the products where cleaned and concentrated using the Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... and RNA was extracted from each replicate using the MasterPure Yeast RNA Purification Kit (Epicentre). Integrity ...
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bioRxiv - Microbiology 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Systems Biology 2020Quote: ... and removed stable RNAs by using the RiboZero Gram Positive ribosomal RNA depletion kit (Epicentre). We prepared the sequencing libraries using the TruSeq stranded Total RNA kit (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification and precipitation of RNA were performed using the MasterPure RNA Purification Kit from Epicentre by adding 200 μl of 2X T and C Lysis Solution and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Random knockout mutants were produced using the EZ::Tn5Tm
Tnp TransposomeTm kit (Epicentre) following the manufacturers recommendations ... -
bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the Quantseq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in two steps ...
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bioRxiv - Cell Biology 2019Quote: ... and in vitro transcription performed using the T7-Flash BiotinRNA Transcription Kit (Epicentre, biotin labelling) or TranscriptAid T7 High Yield Transcription Kit (ThermoScientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... shRNAs were synthesized with AmpliScribe™ T7-Flash™ Transcription Kit (Epicentre, Charlotte, NC, USA) for 15 h followed by 15 min treatment with 1 μl of DNAse I ...
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bioRxiv - Molecular Biology 2020Quote: Transposon mutant libraries were constructed using the EZ-Tn5
TnP Transposome Kit (Epicentre) according to the manufacturer’s instructions ... -
bioRxiv - Genetics 2020Quote: ... for qRT-PCR and northern blotting and MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) for RNA-seq ...
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bioRxiv - Genetics 2020Quote: ... for qRT-PCR and northern blotting and MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) for RNA-seq ...
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Intra-species transcriptional profiling reveals key regulators of Candida albicans pathogenic traitsbioRxiv - Genomics 2021Quote: ... RNA was harvested from cells using a Masterpure Yeast RNA Purification kit (Epicentre, Madison, WI) and treated with DNaseI (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and then ribosomal RNA was removed by a Ribo-Zero Magnetic kit (Epicentre Biotechnologies, USA). RNA quality was checked by NanoDrop (ThermoFisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... RNA isolation was performed according to the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... RNA isolation was performed according to the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific RNA-Seq libraries were synthesized using a Plant Leaf ScriptSeq Complete Kit (Epicentre). Libraries were sequenced for 2 × 100-bp reads on an Illumina HiSeq 2500 ...
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bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Microbiology 2022Quote: ... In vitro transcription was performed using AmpliScribe T7-Flash Transcription kit (Lucigen Corp., Middleton, USA), in the presence of 32P-labeled nucleotides ...
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bioRxiv - Microbiology 2023Quote: Directional libraries were prepared with the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) and single end sequencing was performed on a Hi-Seq 2000 platform at the Deep Sequencing Core Facility at the University of Massachusetts Medical School in Worcester ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA was depleted from 10μg total RNA using the Ribo-Zero Magnetic Kit (Epicentre). Ribo-depleted RNA was fragmented ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and ribosomal RNAs were depleted using the Gram-negative bacteria RiboZero rRNA Removal Kit (Epicentre). Final eluates were used as input for strand-specific RNA-seq library construction using the NEBNext RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... we utilized the ScriptCap m7G Capping System and ScriptCap 2’-O-methyltransferase kit (Epicentre Biotechnologies) with [α-32P] GTP for our experiments ...