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Citations for Lucigen :
801 - 850 of 1243 citations for DNA Damage 8 OHdG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Total RNA was extracted using MasterPure yeast RNA purification kit (Epicentre). To completely eliminate DNA contamination ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared with the ScriptSeq v2 kit (Epicentre) and subjected to paired-end sequencing (2 x 100 bp ...
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bioRxiv - Microbiology 2019Quote: ... and the Ribo-Zero rRNA removal kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: ... Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre). RNA fragmentation ...
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bioRxiv - Cancer Biology 2019Quote: ... then 0.5 μg were treated with Ribozero rRNA removal kit (Epicentre) and EtOH precipitated ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA libraries were then prepared using ScriptSeq v2 Stranded Kit (Epicentre) following the manufacturer’s protocol except fragmentation was done at 85°C for 8min ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was prepared using the MasterPure RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... rinsed once with 1x phi29 polymerase buffer (Lucigen, NxGen kit 30221) in nuclease-free water ...
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bioRxiv - Microbiology 2022Quote: ... 16S and 5S rRNA using the RiboZero rRNA Removal Kit (Epicentre) (two times) ...
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bioRxiv - Microbiology 2022Quote: ... sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre), DNase treated ...
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bioRxiv - Genomics 2022Quote: ... rRNA was removed using a Ribo-Zero kit (Epicentre, Madison, WI) and mRNA was randomly fragmented ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the ScriptSeq v2 kit (EpiCentre). Total RNA was depleted using RiboZero Gold (EpiCentre ...
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bioRxiv - Molecular Biology 2024Quote: ... after ribodepletion with the Ribo-Zero Magnetic Kit for yeast (Epicentre) and sequenced on an Illumina HiSeq 4000 instrument.
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bioRxiv - Molecular Biology 2019Quote: ... 1 µl 50 mM MnCl2 was added to the samples together with 1 µl CircLigase (EPICENTRE). Reaction was carried out for 1h at 60°C and the enzyme was inactivated by further incubation for 10 min at 80°C ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting ligation mixture was electroporated into TG-1 phage-display competent cells (#60502-1, Lucigen) and plated onto 2xYT agar containing 100 µg/mL carbenicillin and 2% (w/v ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Genomics 2020Quote: ... clones were genotyped using PCR primers outside of the gRNA targeted region (Table S6) to identify clones homozygous for the mariner deletion (DNA QuickExtract Lucigen #QE09050). Clones passing initial genotyping were then further expanded ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed once with DPBS and genomic DNA (gDNA) was extracted with 50 µL/well of Quick-Extract solution (Lucigen, QE09050). Lysates were pipetted up and down thoroughly ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was spun down at 5,000 x g for 5 minutes and the pellet was resuspended in 100 µl of QuickExtract™ DNA Extraction Solution (Lucigen) and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre ...
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bioRxiv - Neuroscience 2019Quote: ... The genotype of Tet2flox/flox;Cx3Cr1WT/WT and Tet2flox/flox;Cx3Cr1Cre/WT mice was determined by analysis of DNA extracted from the fingers using a QuickExtract™ (Epicentre) and amplified with MyTaq™ Red DNA Polymerase (Bioline) ...
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bioRxiv - Neuroscience 2019Quote: ... The deletion of the Tet2 gene was determined by analysis of DNA extracted from isolated primary microglia using a QuickExtract™ (Epicentre) and amplified with MyTaq™ Red DNA Polymerase (Bioline).
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Genetics 2021Quote: ... cloni 10G supreme (#60081-1; Lucigen). Each library was grown overnight in 500 ml of standard Luria Broth (LB ...
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bioRxiv - Genomics 2019Quote: ... 1 μL Reverse PCR Primer (Epicentre), 1 ng of cDNA library ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 4 μl Actinomycin D (500ng/μl ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 2 μl Maxima H- RTase (EP0751 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen) and 31 μl water ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 U/ml Rnase Inhibitor (Lucigen), 2.5 μM Template Switch Oligo ...
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bioRxiv - Genomics 2021Quote: ... 6uL 100mM MgSO4 (Lucigen, F98695-1), 10uL Betaine (Lucigen ...
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bioRxiv - Genomics 2021Quote: ... 1 ml of recovery media (Lucigen) was added ...
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bioRxiv - Genomics 2021Quote: ... 1 ml of recovery media (Lucigen) was added ...
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bioRxiv - Systems Biology 2023Quote: ... cloni 10G supreme (#60081-1; Lucigen).
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bioRxiv - Systems Biology 2023Quote: ... cloni 10G supreme (#60081-1; Lucigen). To estimate the approximate complexity of the resulting libraries ...
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bioRxiv - Biophysics 2023Quote: ... coli competent cells (Lucigen, #60442-1) were transformed with the plasmid and then 2 L Terrific Broth supplemented with ampicillin was inoculated and grown at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CircLigase II buffer (Lucigen, CL9021K), and 2.5 mM MnCl2 was added to 16 µL of cDNA and incubated at 60°C for 100 min followed by 80°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
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bioRxiv - Biochemistry 2022Quote: ... Clones with the best response to TMP were expanded and lysed for gDNA extraction and purification using the QuickExtract DNA extraction solution (Lucigen, Middleton, WI). Genomic ecDHFR was amplified (forward ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). The genotyping primers were as described previously29,30,92
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bioRxiv - Molecular Biology 2020Quote: The >200nt RNA fraction was ribo-depleted using non-overlapping DNA oligonucleotides that are complementary to rRNA 18s and 28s followed by digestion with Hybridase™ Thermostable RNaseH (Lucigen #H39500), as previously described (79) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 22 Alanine production plasmids were constructed using codon optimized (Codon Optimization Tool from the IDT) synthetic DNA and the pSMART-HCKan vector (Lucigen, Middleton, WI). Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.
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bioRxiv - Genomics 2022Quote: ... Genomic DNA from edited and unedited HSPC samples was harvested 48h post-editing using QuickExtract DNA Extraction Solution according to manufacturer’s recommendations (Lucigen Corp., Teddington, UK) and diluted to 4.55 ng/uL in IDTE pH 8.0 (IDT ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was randomly sheared through a syringe needle and was end-repaired and cloned into pWEB::TNC (Epicentre Technologies, Madison, WI), followed by packaging into MaxPlax lambda packaging extracts ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). For embryonic genotyping during primary culture preparation ...
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bioRxiv - Genetics 2021Quote: ... and libraries prepared using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre), PCR amplified in 9 cycles ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were then synthesized using the AmpliScribe T7-Flash Transcription Kit (Lucigen) and purified by ethanol precipitation or with RNeasy MinElute Cleanup Kit (QIAGEN) ...
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bioRxiv - Genomics 2020Quote: ... RNA extraction was done using the ‘MasterPure Yeast RNA Purification Kit’ (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The AmpliScribe™ T7 High Yield Transcription Kit was purchased from Epicentre and the HiScribe T7 In Vitro Transcription Kit was obtained from New England Biolabs ...
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bioRxiv - Immunology 2019Quote: ... and depleted of ribosomal-RNA (Ribo-Zero™ rRNA Removal Kit, Epicentre) after purification ...