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Citations for Lucigen :
501 - 550 of 1243 citations for DNA Damage 8 OHdG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from bulk cells transfected with CRISPR/Cas9 plasmids using the epicentre QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Cell samples were grown to mid-log in rich media and DNA was collected using a MasterPure Gram Positive DNA (Epicentre) purification kit with modifications as previously described [53] ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells from each of these 4 wells were dissociated and divided into 2 portions–one portion was used to collect genomic DNA using QuickExtract DNA Extraction solution (Lucigen) while 500 cells from the other portion was suspended in 25 ml of culture medium and 100 ul was seeded into each well of two 96-well plates for further culture ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA of Pkd1-/-or Pkd2-/- single cell clonal mNPC lines were extracted using QuickExtractTM DNA Extraction Solution (Lucigen, # QE09050). PCR primers were designed to flank the sgRNA targeting sites of Pkd1 or Pkd2 genes (listed below) ...
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bioRxiv - Genomics 2023Quote: ... the media was removed and genomic DNA was extracted using 30 uL/well of QuickExtract DNA Extraction Solution (Lucigen #QE09050) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Bioengineering 2019Quote: ... genomic DNA was extracted using QuickExtract Solution (Epicentre), and genomic loci were amplified by PCR utilizing the Phusion Hot Start Flex DNA Polymerase (NEB) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... QuickExtract DNA Extraction Solution was purchased from Lucigen Corporation (WI ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA samples were prepared using QuickExtract (Epicentre). The PCR products were column purified and analyzed with Surveyor Mutation Detection Kit (IDT) ...
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bioRxiv - Neuroscience 2020Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen) followed by PCR and Sequencing to determine efficient rescue.
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bioRxiv - Cancer Biology 2019Quote: ... DNA was isolated from clones with QuickExtract (Epicentre) for genotyping and replica plated for clonal expansion ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by chromosomal DNA isolation (Quick Extract /Lucigen), PCR amplification (Sup Table 8 ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: Genomic DNA was extracted with QuickExtract buffer (Lucigen) by adding 30 µL to a single well of a 96-well plate and incubating for 5 min ...
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bioRxiv - Immunology 2020Quote: ... We digested the DNA with Quickextract (Lucigen Corporation) at 68°C for 90 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient repair of the mutation.
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bioRxiv - Microbiology 2022Quote: ... and DNA was extracted using GELase treatment (Epicentre Biotechnologies ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using Quickextract solution (Lucigen). A ∼700 bp-1 kb region flanking the sgRNA annealing site was amplified by PCR 5-7 days after RNP nucleofection ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted using QE buffer (Lucigen) and PCR was performed using Q5 polymerase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted with QuickExtract (Lucigen #QE09050) for amplicon sequencing.
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bioRxiv - Evolutionary Biology 2023Quote: ... the QuickExtract™ DNA Extraction Solution method (Lucigen), or a salt extraction method (Aljanabi & Martinez ...
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bioRxiv - Genetics 2023Quote: ... 4 units of Ampligase® DNA Ligase (Epicentre), 0.2 ul of Ampligase® 10X Reaction Buffer ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was isolated using QuickExtract solution (Lucigen). Reactions were incubated for 10 min at 65 °C followed by 5 min at 98 °C and cooling to 4 °C ...
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bioRxiv - Genomics 2019Quote: ... The sample was placed on ice and preincubated with the CircLigase II reaction buffer on ice for 5 min (Nuclease-free H2O, 1 × CircLigase buffer, 2.5 mM MnCl2, 0.5 M Betaine, [all included in kit from CL9025K, Epicentre]). After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomal RNA was depleted from 1 µg of total RNA using the Ribo-Zero rRNA Removal Kit (Epicentre) for Plants and Bacteria ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
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bioRxiv - Developmental Biology 2023Quote: ... A piece of embryonic tail was taken from each embryo and DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from 2.5×105 Neuro2A cells with 250 μL of QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA) or from 30 mg of mouse liver using a EZ-10 Spin Column Animal Genomic DNA Miniprep Kit (Bio Basic ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA for CRISPR/Cas9 genotyping was isolated from cell lines and organoids by using Quick Extract DNA Extraction Solution (Lucigen, #QE09050). For organoids ...
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bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was extracted after 48-72 hrs incubation using 50 µL Quick Extract™ DNA Extraction Solution (Lucigen, Middleton, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Stranded RNA-seq libraries were prepared from 1 μg total RNA using ScriptSeq v2 Complete kit for bacteria (Epicentre) and sequenced on Illumina NovaSeq 6000 platform at GENEWIZ ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a rolling-circle amplification (RCA) reaction mixture (1 unit/μL phi29 polymerase (Lucigen, NxGen kit 30221), 1x phi29 polymerase buffer ...
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bioRxiv - Cell Biology 2019Quote: ... it was extracted (QuickExtract DNA extraction solution, Epicentre Biotechnologies), the region of interest was amplified by PCR (primers described in Supplemental Table 4) ...
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bioRxiv - Microbiology 2021Quote: ... swinhoei Y total DNA in the pWEB vector (Epicentre), was screened and a 3D growth-and-dilution library format as reported earlier45 ...
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bioRxiv - Genomics 2022Quote: ... 16 U of DNA Ampligase (Epicentre Technologies, Madison, WI), 0.016 mM dNTP ...
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bioRxiv - Genomics 2022Quote: ... 1x Ampligase DNA ligase buffer (Epicentre Technologies, Madison, WI), 0.5 μl of 5M Betain (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA for clone screens was extracted by QuickExtract (Lucigen) or ethanol precipitation.
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bioRxiv - Genetics 2020Quote: ... Worms were lysed in DNA Quick Lysate (Epicentre Technologies) for 1 hour at 60°C and the lysate was then used as a template for PCR with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: 30 µl of Quick Extract DNA Estraction (Lucigen, QE09050,) was added to 5.0 x 104 pelleted iPSC ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was extracted using the DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient mutation rescue.
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bioRxiv - Genomics 2023Quote: ... 1µl Fast-Link DNA ligase (Lucigen, cat. no. LK6201H), 3 µl 10× Fast link ligation buffer and 1mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: QuickExtract DNA Extraction Solution (Lucigen, cat. No. LGN-QE09050)
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... blunt-end ligation into the pZE21 MCS1 expression vector with constitutive promoter pLteo-1 (Lutz and Bujard, 1997) at the HincII site using the Fast Link ligation kit (Epicentre), and iv ...
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bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...