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Citations for Agilent :
351 - 400 of 6626 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The REAL™ EnVision™ Detection system including anti-rabbit/mouse IgG secondary antibodies and peroxidase/diaminobenzidine (Dako, Agilent Technologies) was used to visualize the primary antibody‑binding cells according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The REAL™ EnVision™ Detection system including anti-rabbit/mouse IgG secondary antibodies and peroxidase/diaminobenzidine (Dako, Agilent Technologies) was used to visualize the primary antibody‑binding cells according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: RT-PCR was performed in a Stratagene™ Mx3005P qPCR instrument (Agilent Technologies, Waldbronn, Germany) using 10 μl QuantiTec SYBR Green PCR mix (Qiagen) ...
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bioRxiv - Biochemistry 2019Quote: DSF was performed in a 96-well plate using an Mx3005p RT-PCR machine (Stratagene). Each well (20 µl ...
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bioRxiv - Physiology 2022Quote: ... qPCR analysis was performed using the Stratagene MX3000P RT-PCR System (Stratagene, La Jolla, CA) in a 25-μL reaction mixture ...
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bioRxiv - Biochemistry 2020Quote: DSF was performed in a 96-well plate using an Mx3005p RT-PCR machine (Stratagene) with excitation and emission filters of 492 and 610 nm ...
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bioRxiv - Immunology 2022Quote: ... the sections were stained with DAB detection kit (Dako, Copenhagen, Denmark) and hematoxylin ...
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bioRxiv - Immunology 2019Quote: Real-time oxygen consumption rate (OCR) in macrophages was determined with an XFp Extracellular Flux Analyzer (Seahorse Bioscience). The assay was performed in XF Assay Modified DMEM using 1.6×105 cells/well and 3 wells per condition ...
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bioRxiv - Molecular Biology 2023Quote: Mitochondrial respiration (OCR and ECAR) was quantified in real-time using the Seahorse extracellular XFe96 flux analyzer (Agilent) for cells from primary skin fibroblast ...
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bioRxiv - Neuroscience 2022Quote: ... 3x 5 minutes) and incubated in the secondary antibody-horseradish peroxidase (HRP) complex as part of REAL EnVision detection system (Dako #K5007) for 1h at RT ...
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bioRxiv - Biochemistry 2020Quote: Human ISG15 cDNA was amplified by RT-PCR from universal human reference RNA (Agilent 740000-41), adding BamHI and NotI restriction sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Cell Biology 2019Quote: The mitochondrial OCR and ECAR were monitored in vivo in real-time using a Seahorse XF analyzer (XFe24, Agilent). Depending on the experiment ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed with a minimum of three biological replicates per gene using THUNDERBIRD SYBR qPCR Mix (TOYOBO) according to the manufacturer’s instructions and ran on Mx3000P Real-Time QPCR System (Agilent). Analysis was carried out using double CT method ...
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bioRxiv - Molecular Biology 2022Quote: Real-time cell metabolic profiling of gastric cell lines was performed with a Seahorse XF HS Mini Analyzer (Agilent). Cells were seeded at the following densities ...
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bioRxiv - Cancer Biology 2023Quote: xCELLigence experiments were performed using the Real-Time Cell Analyzer (RTCA) Dual Plate (DP) instrument according to the manufacturer’s instructions (Agilent.com). Briefly ...
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bioRxiv - Cell Biology 2023Quote: Oxygen consumption rates of primary neurons were measured in real-time in an XFe24 Extracellular Flux Analyzer (Seahorse Bioscience; Seahorse Wave Desktop software 2.6.1.56) ...
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bioRxiv - Microbiology 2021Quote: ... For secondary antibody incubation and signal detection LSAB and AEC Kits (DakoCytomation) were used following the manufacturers protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The IHC staining was performed with a diaminobenzidine (DAB) detection kit (Dako). The multiplexed IF staining was performed following the instruction of Opal 7-color IHC kit (NEL811001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... tissues were incubated with secondary antibodies (EnVision Chem Detection Kit, DaKo Cytomation) at room temperature for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... the sections were stained with a DAB detection kit (Dako, Copenhagen, Denmark) and hematoxylin ...
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bioRxiv - Microbiology 2019Quote: ... and retention time (RT) were imported into a personal compound database and library (PCDL Manager, version B.07.00, Agilent Technologies) used in data processing workflow.
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bioRxiv - Immunology 2019Quote: ... Quantification of the purified RT-PCR products was also performed using an Agilent 2100 Bioanalyzer (Agilent Technologies) according to the Agilent High Sensitivity DNA kit instructions.
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bioRxiv - Biochemistry 2019Quote: ... Plates were sealed using optically clear films and transferred to an Agilent Mx3005p RT-PCR machine (Agilent). The fluorescence intensity was measured during a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR reaction targeting RdRP gene was performed in triplicate in a Mx3005P QPCR System (Agilent) using PrecisionFAST qPCR Master Mix (PFAST-LR-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... For detection the EnVision detection system (K500711-2, Dako) was used.
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bioRxiv - Developmental Biology 2020Quote: ... The signals were visualized using a REAL Streptavidin Alkaline Phosphatase kit (Dako), while Ki-67 staining was visualized with DAB ...
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bioRxiv - Genetics 2022Quote: PCR products were cloned using StrataClone PCR Cloning Kit (Agilent, Waldbronn, Germany). Positive white colonies were randomly picked and verified with colony PCR ...
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bioRxiv - Physiology 2020Quote: ... Real-time qPCR amplification was performed using SYBR GreenER™ qPCR SuperMix Universal (Thermo) or miRNA QPCR Master Mix (Agilent).
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bioRxiv - Neuroscience 2019Quote: ... Real-time verification of the DBS stimulation parameters was monitored using a digital oscilloscope (Agilent 54622D oscilloscope, Agilent Technologies, Amstelveen). Animals requiring stimulation intensities above 100 µA were excluded from the analysis ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed using a Thunderbird SYBR qPCR Mix (Toyobo) and the analysis was carried out with a real-time thermal cycler (Stratagene Mx3000p; Agilent). Primers for quantitative RT-PCR are as follows ...
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bioRxiv - Developmental Biology 2020Quote: ... Gene-specific RT-PCR was performed using the SYBR green master mix (Applied Biosystem) and Agilent MX3005P Multiplex QPCR Real-time Thermal Cycler (Stratagene). The amplification protocol consisted of an initial cycle of 1 min at 95°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time qPCR amplification was performed using SYBR GreenER™ qPCR SuperMix Universal (Thermo) or miRNA QPCR Master Mix (Agilent). The miR-467a primer (GTA AGT GCC TAT GTA TATG ...
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bioRxiv - Immunology 2019Quote: ... Microplates were incubated for 30 min at 37 °C in a non-CO2 incubator and subjected to real-time analysis of OCR using an XF96 Extracellular Flux Analyzer (Agilent). For the mitochondrial stress assay analysis ...
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bioRxiv - Immunology 2021Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using the real-time flux analyzer Seahorse XF24 (Agilent) according to a method modified from Van den Bossche et al ...
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bioRxiv - Neuroscience 2022Quote: Mitochondrial and glycolytic function was analyzed with the Seahorse Cell Mito Stress Test and Seahorse Real-Time ATP Rate Assay on a Seahorse XFe24 Extracellular Flux Analyzer (Agilent). Neurons were treated on day 11 of culture with 500 μL of fresh neuronal media plus 500 μL of astrocytic conditioned media of varying treatment ...
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bioRxiv - Cancer Biology 2022Quote: Mitochondrial oxygen consumption rate (OCR) was measured in real-time using the XFp Extracellular Flux Analyzer (Seahorse Bioscience, MA, USA). Cells were seeded in 6-well plates at density 80 000 cells/well and treated with Tranilast (100 µM ...
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bioRxiv - Cancer Biology 2022Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in real-time using Seahorse (XF24 and XFe96) extracellular flux analyzers (Agilent). Cells were seeded in XF24 (5×104 ...
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bioRxiv - Biochemistry 2023Quote: ... drugs were added at the indicated concentration in triplicate and the cell index was measured over a period of 48-96 h (Real Time Cell Analyzer, Agilent).
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bioRxiv - Pharmacology and Toxicology 2023Quote: Measurements of real-time of oxygen consumption rates (OCR) were determined in whole cells using a Seahorse XF96 extracellular flux analyser (Agilent) as described previously (78) ...
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bioRxiv - Cancer Biology 2023Quote: Oxygen consumption was measured using Oxygen consumption rate (OCR) was measured in real time using the XF96 extracellular flux analyzer (Agilent). AML cells were seeded on Cell-Tak (#10317081 ...
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bioRxiv - Plant Biology 2023Quote: The HRM analysis was carried out using MeltDoctorTM HRM Master Mix on AriaMx G8830A Real-Time qPCR System (Agilent Technologies). Each 20 µl reaction comprised of 10 µl Evagreen HRM master mix ...
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bioRxiv - Neuroscience 2023Quote: ... HLA-DR-and PLP-stained sections were incubated with HRP-labelled anti-mouse antibody (K5007, Dako Real EnVision detection system; Dako, Nowy Sącz, Poland) for 1 hour and immunostaining was visualized with 3’3-diaminobenzidine (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Biophysics 2023Quote: ... PCR site directed mutagenesis kit (Agilent Technologies) was used according to the instruction manual for mutagenesis PCR.
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative RT-PCR and relative quantification analyses were performed with the MX3000P system (Agilent, Santa Clara, CA, USA) using SYBR Green dye chemistry ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNAs were obtained and amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Detection was achieved using the Envision rabbit detection system (Dako) with diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed by using QuantiNova kit on Mx3500P PCR machine (Stratagene). The following oligonucleotide primers were used to detect target gene expression ...
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bioRxiv - Plant Biology 2021Quote: ... Chimeric PCR products were subcloned before sequencing using StrataClone PCR cloning kits (Agilent).
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was carried out using Thunderbird SYBR qPCR Mix (Toyobo, Japan) with a Stratagene mx 3000p real-time thermal cycler (Agilent, USA). Relative transcript levels were calculated against a standard curve with normalization to the expression of PLANT U-BOX PROTEIN1 (PUB1 ...