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Citations for Agilent :
601 - 650 of 6626 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: The SrIRED gene was amplified with error-prone PCR using the GeneMorph II random mutagenesis kit (Agilent) following the instructions of the manufacturer using 2.4 µg template plasmid and 25 PCR cycles ...
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bioRxiv - Microbiology 2019Quote: ... and single-stranded cDNA was synthesized by AffinityScript RT-RNAse (Stratagene). cDNA synthesized without the RT/RNAse block enzyme mixture was used to control for genomic DNA contamination ...
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bioRxiv - Microbiology 2021Quote: ... a 10 min incubation at RT with peroxidase blocking buffer (Agilent) and a 30 min incubation at RT with Envision+System HRP Rabbit (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: ... The RT was performed using a thermociclator Stratagene Mx3005P (Agilent Technologies) with the following steps ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR reactions were carried out in AriaMX (Agilent, CA, USA). Reactions were set up in 1X PowerUP SYBR Green master mix (Cat No ...
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bioRxiv - Microbiology 2023Quote: ... a 10 min incubation at RT with peroxidase blocking buffer (Agilent) and a 30 min incubation at RT with Envision+System HRP Rabbit (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... Quadrupole time-of-flight mass-spectrometry (Agilent) of RNase treated and re-purified AbmR was completed at the University at Albany ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Biophysics 2021Quote: The error-prone PCR reaction for libraries “SH3_EP” was done using the GeneMorph II Mutagenesis Kit (Agilent Technologies) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Bands were excised and purified for cloning with the StrataClone PCR Cloning kit (#240205, Agilent, Santa Clara, CA), following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... Mutations were introduced using standard PCR or using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, 210515). Double A3H fusion constructs I-I ...
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bioRxiv - Biophysics 2021Quote: ... and L207W mutations were generated by performing site-directed mutagenesis PCR using Quik Change II Mutagenesis Kit (Agilent) with the following primers:
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bioRxiv - Synthetic Biology 2020Quote: ... The assembly products were then re-purified using the Monarch PCR & DNA Cleanup Kit and quantified by Agilent Bioanalyzer (DNA 1000).
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bioRxiv - Immunology 2021Quote: ... RACE products were subjected to gel-purification and sub-cloned using the Strataclone UA PCR cloning kit (Agilent). Insert sequences were determined by Sanger sequencing (Seqlab GmbH).
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bioRxiv - Immunology 2022Quote: ... sections were incubated with Dako REAL peroxidase blocking solution (Dako, K0672) to inactivate endogenous peroxidase and Fab fragment-goat-anti-mouse IgG (JacksonImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... overnight at 4°C in Dako REAL antibody diluent (Dako, S2022). To visualized the specific signal ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were treated with Dako REAL peroxidase block solution (Agilent; #S2023) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by colour development using DAB (DAKO REAL™ EnVision ™) as chromogenic substrate for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... Target detection was performed using DAB+chromogen (Agilent Technologies, Santa Clara, USA). Slides were counterstained with hematoxylin Gill’s Formula (H-3401 ...
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bioRxiv - Biochemistry 2021Quote: ... Peak detection and integration were performed using MassHunter (Version B07.01 SP2, Agilent).
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bioRxiv - Microbiology 2021Quote: ... An HRP-labelled polymer detection reagent (EnVision+ System - HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Neuroscience 2019Quote: ... the secondary antibody containing HRP rabbit/mouse serum (DAKO EnVision Detection Systems) was added and incubated for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... A DAB substrate buffer solution was subsequently added (DAKO EnVision Detection Systems) and incubated for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Signals were detected using Envision-plus detection system (Dako, Carpinteria, CA, USA) and visualized following incubation with 3,3′-diaminobenzidine.
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bioRxiv - Immunology 2023Quote: ... Signal detection was amplified using an Agilent GenPoint staining platform (Agilent Technologies) and visualized by DAB colorimetric reaction ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were dried at RT and mounted in Fluorescence Mounting Media (Dako). TUNEL staining was carried out on PFA-fixed sections using the In Situ Cell Death Detection Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... the ligated product was reverse transcribed using Affinity Script RT enzyme (Agilent) and a primer complementary to the ligated adapter ...
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bioRxiv - Molecular Biology 2022Quote: ... First strand of cDNA is synthesized using AffinityScript RT Enzyme (Agilent, 600107) according to the manufacturer’s instructions at 54? for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... for 1.5h at RT and HRP-conjugated rabbit anti-rat (Agilent, P0450) for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... Ligated product was reverse-transcribed using the Affinity Script RT enzyme (Agilent) and a primer complementary to the ligated product ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cloned into StrataClone PCR Cloning vector (Agilent, #240205), linearized with XbaI restriction enzyme (Takara ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We produced the promoter inserts by performing error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). We used the plasmid constructs with MG1655 promoter variants cloned into them as template DNA for the error-prone PCR aiming to achieve approximately 1.5 SNPs per promoter sequence ...
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bioRxiv - Developmental Biology 2019Quote: ... Arg-177-Ala mutants were generated in pCR II-TOPO TA vector using QuikChange site directed mutagenesis kit (Agilent). The wild type and the respective modifications were confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were cleaned up with SPRIselect beads and quantified using Qubit dsDNA HS assay kit (Agilent Technologies). The libraries were sequenced on a HiSeq2500 with paired-end 50-bp reads (Illumina).
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bioRxiv - Evolutionary Biology 2022Quote: ... We produced the promoter inserts by performing error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). We used 25 ng of the plasmid construct with the MG1655 lacZ promoter variant cloned into it as a template DNA for the error-prone PCR to achieve approximately 1.5 SNPs per variant sequence ...
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bioRxiv - Genomics 2022Quote: ... The bisulfite-converted library was PCR amplified and indexed using the SureSelect XT Mouse Methyl-Seq Kit (Agilent, #G9651A). Prepared libraries were run on an Illumina sequencing platform using a NextSeq High 150 bp paired-end mode.
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bioRxiv - Evolutionary Biology 2022Quote: ... PKR site-specific mutants and epteK3Δ227-508 were generated by PCR mutagenesis using the QuickChange Lightning mutagenesis kit (Agilent) and primers holding the desired mutations/deletions ...
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bioRxiv - Cell Biology 2020Quote: ... P190RhoGAP-A mutants were produced through PCR-based site-directed mutagenesis using the Quikchange kit (Stratagene, San Diego, CA). For the Y1087F mutation ...
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bioRxiv - Immunology 2020Quote: ... and subsequently mutagenized by error-prone PCR (ePCR) via the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Cat # 200550) with a target nucleotide mutation frequency of 0–4.5 mutations per kilobase of DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified PCR products were quantified using Agilent Bioanalyzer 2100 Instrument using the High sensitivity DNA Kit (Agilent, 5067-4626). Ion Torrent Emulsion PCR and enrichment steps were performed using Ion PGM HiQ View OT2 kit (Life technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were detected by polyacrylamide gene electrophoresis or the Agilent DNA 1000 kit (Agilent Technologies Inc, Germany). Samples from the Innsbruck cohort and the remaining non-HPV16/18 samples from the Oslo cohort (n=40 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were cloned into the StrataClone TA-cloning vector and transformed into StrataClone SoloPack competent cells according to the manufacturer’s protocol (StrataClone PCR Cloning Kit, Agilent), resulting in pFF-162 (SpoY) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were assessed for purity and concentration on the Bioanalyzer using the High Sensitivity DNA Kit (Agilent Technologies). Samples were multiplexed and sequenced on the Illumina HiSeq 2500 system (Genome Technology Core ...
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bioRxiv - Bioengineering 2024Quote: ... Error-prone PCR reactions were carried out following the protocol provided by GeneMorph II Random Mutagenesis Kit (200550, Agilent). Primers were designed to flank residue 1 to 119 of the binder ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then blocked in the Peroxidase Blocking solution (Dako REAL, S2023) for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then blocked in the Peroxidase Blocking solution (Dako REAL, S2023) for 8 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR reaction was performed using Brilliant PCR mix (Agilent Technologies, USA). The standard curve was drawn using serially diluted ΔintS genomic DNA ...
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bioRxiv - Neuroscience 2021Quote: ... and the PCR product was subcloned into Strataclone blunt PCR vectors (Stratagene) and subcloned further into pCM V-ONCM to generate the pCMV-SS-ONCM construct ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by colourimetric detection by diaminobenzidine substrate (Dako, Santa Clara, CA, USA, #K3468). Following this ...