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Citations for Agilent :
201 - 250 of 1356 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... and immunolabelled for human CD31 (Dako, JC70A, 1:50), VE-Cadherin (1:100 ...
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bioRxiv - Immunology 2023Quote: ... human albumin (diluted 1:1000; Dako, Carpinteria, CA, USA), complement C3 (diluted 1:1000 ...
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bioRxiv - Genomics 2020Quote: ... HindIII-based Hi-C libraries from our previous study (Wutz et al., 2017) were captured using SureSelect target enrichment system (Agilent Technologies) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... the eight genes flanked with the same promoters and terminators were cloned into the 2μ-based high-copy plasmids pESC-URA-USER and pESC-HIS (#217451, Agilent Technologies), resulting in pESC-URA-USER harboring CYP79A2 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Each Hi-C library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment ...
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bioRxiv - Evolutionary Biology 2021Quote: Evolved viral genomes of the passage 12 from each lineage and genotype were amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were subjected to C18 desalting using an Agilent 1260 Infinity LC system equipped with an MRP-C18 Hi-recovery column (Agilent, USA) before SpeedVac drying.
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bioRxiv - Microbiology 2022Quote: ... and analysed by HPLC Shimadzu, Prominence-I (Milton Keynes, UK) with an ion exchange column Hi PlexH (300 × 7.7 mm) (Agilent, Cheadle, UK) and 0.6 mL min-1 flow rate at 55 ºC (oven temperature ...
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bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl C18 Cartridges (Agilent) were used as solid phase ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit anti-human tau was from Dako (1:8000, A0024). Other tau antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) for human CD45 antibodies (IR75161-2, Agilent Technologies) was performed on FFPE blocks of engrafted tumors to identify cases of lymphomagenesis ...
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bioRxiv - Immunology 2022Quote: ... the plates were incubated with either anti-human-HRP (Dako), anti-rat-HRP (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... (30) using the SureSelectXT Human All Exon 50 Mb (Agilent) bait set ...
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bioRxiv - Cancer Biology 2020Quote: ... monoclonal mouse anti-human alpha smooth muscle actin (M0851, Dako), monoclonal mouse anti-human CD68 (M0814 ...
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bioRxiv - Cancer Biology 2021Quote: ... or 1:400 diluted Rabbit anti-human calcitonin (Dako, Denmark) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were analyzed against the Stratagene Universal Human Reference (Agilent). Raw fluorescence intensities were quantified and normalized (Lowess normalization ...
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bioRxiv - Cell Biology 2021Quote: ... IgA heavy chain (rabbit anti-human, Dako, A0262 1:1000) and secretory component/pIgR (goat anti-human ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-SMA (1:67, human, Dako, M0851, Santa Clara, CA) with secondary donkey anti-mouse Cy3 (1:200 ...
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bioRxiv - Immunology 2020Quote: ... 0.006 g/L polyclonal rabbit anti-human CD3 antibody (Dako A0452 ...
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bioRxiv - Cancer Biology 2020Quote: ... whole Human Genome 44 K arrays (Agilent Technologies, product G4112A) were used for stroma and epithelial expression profiles ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal CD31 anti-human clone JC70A (M0823, Agilent Dako, Santa Clara ...
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bioRxiv - Genetics 2022Quote: ... including A sample (Universal Human Reference RNA, Agilent Technologies, Inc.) and B sample (Human Brain Reference RNA ...
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bioRxiv - Immunology 2023Quote: ... stimulation with rabbit anti-human IgE (Dako, Carpinteria, CA, USA) and fMLP (N-Formylmethionine-leucyl-phenylalanine ...
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bioRxiv - Immunology 2023Quote: ... and mouse anti-human CD68 (Dako #M0814, 1:400 dilution), following by the secondary antibody incubation and chromogenic detection with DISCOVERY Purple and Yellow kits (Ventana-Roche Diagnostics ...
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bioRxiv - Cancer Biology 2024Quote: ... The Agilent SureSelect Human All Exon v6 kit (Agilent Technologies) was used for whole exome sequencing ...
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bioRxiv - Genetics 2024Quote: ... The exomes of the subjects were captured by Agilent SureSelect Human All Exon V6 Enrichment kits (Agilent, CA, USA) and then sequenced on a HiSeq X-TEN system (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Biochemistry 2020Quote: All mutations in C100 FLAG were introduced by site-directed mutagenesis (QuickChange Lightning Site Directed Mutagenesis kit, Agilent) in pET 22b vector ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Developmental Biology 2020Quote: ... Sox11-FLAG mutants were created by site-directed mutagenesis via polymerase chain reaction with 2XPfu DNA polymerase (Agilent). Primers can be found in Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... and p-dsbS(S239A)- flag were obtained using the QuikChange II site-directed mutagenesis kit (Stratagene, catalog#: 200518). For generating p-dsbR(D51A)-flag ...
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bioRxiv - Cell Biology 2023Quote: ... an asparagine at position 1326 of hsNUDT9H-FLAG (DsRed) was replaced with aspartate using QuikChange mutagenesis system (Agilent). Defined oligonucleotides in sense and antisense configuration were obtained from (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: ... containing an AgeI restriction site in the FLAG sequence created by site-directed mutagenesis (QuikChange Mutagenesis kit, Agilent) using oligonucleotides detailed in Reagents and Tools table.
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bioRxiv - Immunology 2021Quote: The KA and LALA mutations were introduced to the Fc fragment of 2219 by QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) according to the instruction manual ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were first loaded onto a C18 trap column (5 µm, 5 × 0.3 mm, Agilent Technologies) and then eluted into a C18 analytical column (75 μm × 150 mm ...
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bioRxiv - Cell Biology 2020Quote: ... The EXD2-M126A variant was created by site-directed mutagenesis of pDEST-EXD2_iso1-BirA*-FLAG construct using Quick Change Lightning kit (Stratagene).
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Immunology 2020Quote: ... The pcDNA3.1 Flag-STING-C206S construct was generated using the Quikchange Lightning Site-Directed Mutagenesis kit from Agilent (#210518) with the following primer (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... The Flag-tagged PARP1 R138C mutant was generated by the site-directed mutagenesis Kit (Agilent, La Jolla, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, Flag-NLS-linker-Cas9 (Baker et al., 2016) was co-electroporated with a pAAV backbone-based plasmid (Stratagene) containing two U6-promotor-driven gRNAs targeting HPRT (GACTGTAAGTGAATTACTT) ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...