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Citations for Agilent :
1 - 50 of 1283 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected using HRP-labelled rabbit anti–human IgG (Dako) or anti-hamster IgG and TMB (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... and labelled proteins were probed with diaminobenzidine substrate (K3468, Dako) to reveal positive staining ...
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bioRxiv - Biochemistry 2023Quote: ... Same amounts of early and late-labelled DNA were loaded on human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed at 65°C as previously described43 ...
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bioRxiv - Cancer Biology 2021Quote: ... biotin (DakoCytomation), and an additional serum-free protein block (DakoCytomation ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
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bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... An HRP-labelled polymer detection reagent (EnVision+ System - HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were expressed using E.coli BL21 (DE3) RIL according to the manufacturer’s protocol (Stratagene). The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... or HRP-labelled antibodies (DAKO) (P0447 ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Cancer Biology 2021Quote: ... avidin-biotin blockade was performed using the avidin-biotin blocking kit (DAKO, Glostrup, Denmark). To eliminate nonspecific protein interactions with the primary antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Product titer was analyzed offline from spent media daily by protein A chromatography on the Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA). An XCell™ ATF system (Repligen ...
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bioRxiv - Biochemistry 2023Quote: Titer analysis of spent medium was analyzed offline daily by Protein A chromatography on an Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA) as well as with the BLItz biolayer interferometer system (ForteBio ...
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bioRxiv - Genomics 2019Quote: ... incorporating Cy3-labelled CTP (Agilent Technologies), and hybridized onto SurePrint G3 Rat GE 8 x 60K Microarrays v2 (AMADID 074036 ...
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bioRxiv - Biochemistry 2023Quote: ... CLEANEX and DOSY data were collected on 100 μM 15N-labelled protein at a proton Larmour frequency of 600 (Agilent) or 800 (Bruker ...
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bioRxiv - Neuroscience 2020Quote: ... we used the endogenous biotin blocking procedure (Avidin/Biotin Blocking Kit, Dako, code No. X0590) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... biotin and peroxidase blocking reagents (Dako) prior to primary antibody incubation and then with Bio-Rad Amplification Reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... biotin and peroxydase blocking reagents (Dako) before saturation with TBS containing 0.1% Tween-20 and 5% BSA (TBST-BSA) ...
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bioRxiv - Neuroscience 2020Quote: ... Envision + HRP labelled polymer anti-rabbit (DAKO) was used as a replacement of the secondary antibody to enhance the signal ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... then washed and incubated with Labelled polymer HRP-anti-Rabbit and Labelled polymer HRP-anti-mouse secondary antibody (Dako, Denmark) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Tissue samples were stained with hematoxylin and eosin or immunostained to detect SARS-CoV-2 antigen using VIDO-InterVac’s polyclonal rabbit anti-SARS-CoV-2 AS20-014 (1:400 dilution) and detected using an HRP-labelled polymer detection reagent (EnVision+ System – HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Biophysics 2021Quote: ... with second antibody Rb-aMs/biotin (DAKO) for 1h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... followed by avidin and biotin blocking (Dako) before staining with the second tetramer ...
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bioRxiv - Immunology 2021Quote: ... EnVision+ System-HRP Labelled Polymer Anti-rabbit (Agilent), ImmPRESS HRP anti-rat IgG ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with anti-rabbit or anti-mouse horseradish peroxidase- labelled secondary antibody (Dako EnVision+ System- HRP Labelled Polymer) followed by a chemiluminescent reaction (Clarity Western ECL Substrate ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti- mouse biotin (Dako Denmark, Glostrup, Denmark) was used as a secondary antibody and samples were incubated for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and Evision+System-HRP labelled polymer anti-Rabbit (Dako) was applied for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Envision+ System-HRP Labelled Polymer Anti-Rabbit antibodies (Dako) were incubated for 20 min at 32°C ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated with HRP labelled secondary antibodies (Dako) diluted in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... horseradish peroxidase (HRP)-labelled anti-rabbit antibody (Dako K4003) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation with EnVision+ HRP-labelled polymer (Agilent). For visualization ...
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bioRxiv - Microbiology 2019Quote: ... and peroxidase-labelled anti-rabbit EnVision™ secondary antibody (Dako). Following secondary antibody binding ...
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bioRxiv - Neuroscience 2021Quote: ... Labelled sections were mounted in Fluorescence Mounting Medium (#S3023, DAKO) and covered by long coverslips ...
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bioRxiv - Microbiology 2022Quote: ... HRP-labelled goat anti-mouse immunoglobulin (cat. n° P0447, Dako). The following antibodies were used for flow cytometry ...