Labshake search
Citations for Agilent :
251 - 300 of 1283 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... at 4°C overnight followed by incubation with Dako EnVision+System-HRP Labelled Polymer Anti-Mouse (Aglient Dako, Cat # K400111-2) for 60 min at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by incubation for 45 minutes at room temperature in 1:100 dilution of rabbit anti-goat immunoglobulins/HRP (Dako, P0160 or ready-to-use goat anti-rabbit HRP labelled polymer (Dako, K4003). The slides were washed twice in 1x Wash buffer and the color was developed with 3,3-diaminobenzidine tetrahydrochloride (DAB ...
-
bioRxiv - Developmental Biology 2020Quote: ... The sections were washed in TBS and Tween-20 and stained for 1 hour with HRP-labelled secondary antibody (Dako K4003). The staining was developed with diaminobenzidine (DAB ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The fluorescently labelled tissues were placed cut-face-down onto a microslide and were covered in Dako mounting medium (Dako#S302380) to prevent tissue desiccation ...
-
bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
-
bioRxiv - Cancer Biology 2022Quote: ... immunohistochemistry was performed by using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Negative controls were obtained after omission of the primary antibody or incubation with an irrelevant antibody.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... at 4 °C overnight and immunostained using the streptavidin-biotin peroxidase technique (Envision universal peroxidase kit; Dako Cytomation, Milan, Italy). After incubation ...
-
bioRxiv - Cancer Biology 2021Quote: Biopsy sections were deparaffinized and antigens were retrieved by boiling in 10mM sodium citrate buffer pH6 for 40 minutes followed by endogenous biotin blocking (Agilent) and normal goat serum blocking (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2021Quote: ... incubated with Dako biotin (cat. no. X0590) and peroxidase (cat. no. S200389) blocking reagents (both Agilent, Santa Clara, CA, USA), and incubated with primary antibodies (diluted in TBS-T-BSA ...
-
bioRxiv - Cancer Biology 2020Quote: EGFR-KD from Expi293 cell lysates was bound to streptavidin resin and eluted in biotin buffer according to manufacturer’s recommendations (InterPlay Mammalian TAP System; Agilent Technologies). Typical protein yield was between 100-400 ng/µl ...
-
bioRxiv - Molecular Biology 2024Quote: The antigen-antibody complex was detected by the Labeled Streptavidin–Biotin (LSAB) staining method using a biotinylated goat anti-rabbit antibody (DakoCytomation), subsequently conjugated with streptavidin-horseradish peroxidase (HRP ...
-
bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
-
bioRxiv - Genomics 2019Quote: Same amounts of early and late-labeled DNA were loaded on mouse or human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed as previously described (Hadjadj et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Same amounts of early and late-labeled DNA were loaded on human DNA microarrays (SurePrint G3 Human CGH arrays, Agilent Technologies, G4449A). Hybridization was performed as previously described (39) ...
-
bioRxiv - Cell Biology 2020Quote: ... For the anti-human Fibronectin immunostaining (Dako, 1:1000) a single immunostaining was performed with a goat anti-rabbit secondary antibody and Alexa-568 phalloidin ...
-
bioRxiv - Molecular Biology 2021Quote: ... anti-human Chorionic Gonadotropin (hCG, A0231, Dako, 1:300), anti-ENDOU (HAP067448 ...
-
bioRxiv - Genetics 2019Quote: ... using the Cy3-labeled Universal Human Reference cRNA (Agilent). Genes that showed significant change of expression (normalized to DMSO control-treated samples ...
-
bioRxiv - Immunology 2019Quote: ... and rabbit anti-human myeloperoxidase (A039829-2, Dako, USA) at 1:300 ...
-
bioRxiv - Developmental Biology 2019Quote: ... or mouse anti-human CD31 (Dako, M0823, 1:50) overnight at 4°C and then goat anti-mouse Alexa488 (ThermoFisher Scientific ...
-
Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... and with rabbit anti-human IgM (1:250, Dako). Incubation with primary antibodies was followed by a biotinylated goat-anti-rabbit antibody for 30 min followed by the avidin–biotin–peroxidase complex (Vectastain Elite ABC Kit ...
-
bioRxiv - Cancer Biology 2021Quote: ... A Universal Human Reference (UHR) (Chem-Agilent, Catalog #740000) was sequenced with each batch of samples to allow for assessment and removal of technical artifacts (due to ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNA samples were performed to Microarray analysis by Agilent SurePrint G3 human gene expression Microarray 8X60K (Agilent Technologies). After hybridization ...
-
bioRxiv - Genomics 2022Quote: Universal Human Reference RNA (UHRR) was purchased from Agilent (product name ...
-
bioRxiv - Genetics 2022Quote: Preparation of the universal human reference RNA (Agilent, 74000) was done according to the manufacturer’s manual ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... A monoclonal mouse anti-human αSMA antibody (Dako, Denmark) was incubated for 2 hours at room temperature after antigen retrieval ...
-
bioRxiv - Cancer Biology 2020Quote: ... Library preparation (Agilent SureSelect Human All Exon V6 kit) and high throughout sequencing (Illumina NovaSeq 6000 ...
-
bioRxiv - Cell Biology 2021Quote: ... and IgA (rabbit anti-human, Dako, A0262, 1:75). For amplified targets the appropriate HRP-conjugated secondary was then incubated at 1:250 in PBS for 45 minutes-1hr followed by TSA fluorophore treatment (Akoya) ...
-
bioRxiv - Molecular Biology 2021Quote: ... anti-human Chorionic Gonadotropin (CBG, A0231, Dako, 1:1000), anti-ENDOU (HAP067448 ...
-
bioRxiv - Cancer Biology 2020Quote: ... tumor sections were stained for human Ki67 (Dako, #M7240) using the Target Retrieval Solution pH 9.0 (Dako ...
-
bioRxiv - Microbiology 2022Quote: ... Sections were stained with mouse anti human CMV (Dako, Agilent technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal CD31 anti-human clone JC70A (M0823, Agilent Dako ...
-
bioRxiv - Neuroscience 2022Quote: ... wild type human TTC3 in pCMV-Tag2 vector (Stratagene), mouse AUTS2-myc-DDK in pCMV6 (OriGene ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-human CD31 (Agilent, clone DAKO JC70A), mouse monoclonal anti-HLA class II-DR/DP/DQ (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... and immunolabelled for human CD31 (Dako, JC70A, 1:50), VE-Cadherin (1:100 ...
-
bioRxiv - Immunology 2023Quote: ... human albumin (diluted 1:1000; Dako, Carpinteria, CA, USA), complement C3 (diluted 1:1000 ...
-
bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...
-
bioRxiv - Microbiology 2022Quote: ... the plates were incubated with serially diluted serum samples for 2 h at room temperature and antibody binding detected using horseradish peroxidase–labelled rabbit anti-guinea pig antibody (Dako, Glostrup, Denmark) and 5,5’-dithiobis-(2-nitrobenzoic acid) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-mouse IgG (DAKO, cat. P0447) or HRP-labelled polyclonal goat anti-rabbit IgG (DAKO ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-rabbit IgG (DAKO, cat. P0448) or HRP-labelled polyclonal goat anti-mouse IgG (DAKO ...
-
bioRxiv - Cell Biology 2021Quote: ... The pST39-BLOC-1 plasmid encoding recombinant BLOC-1 was transformed into BL21gold(DE3)plysS cells (230134, Agilent). Several colonies from the plate were inoculated into a starter culture of 10 ml LB supplemented with 34 μg/ml chloramphenicol and 100 μg/ml ampicillin that was grown overnight at 37 °C with moderate shaking ...
-
bioRxiv - Neuroscience 2023Quote: Recombinant adeno-associated viruses (AAVs) with serotype 8 were produced using the AAV helper-free system (Agilent Technologies). Human embryonic kidney (HEK ...
-
bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
-
bioRxiv - Microbiology 2020Quote: ... the amount of dT10-PEG2-dT10-10% Biotin strands per NP was estimated using a Varian Cary 100 Bio UV-visible spectrophotometer (Agilent Technologies) and a quartz cuvette of 1 cm path length.
-
bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
-
bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
-
bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
-
bioRxiv - Cancer Biology 2021Quote: ... The amount of Bcl-xL siRNA and KLA in each nanocomplex was calculated by measuring the concentration of Bcl-xL siRNA or fluorescein isothiocyanate (FITC)-labelled KLA (KLA-FITC) in the supernatant before and after the coating using a spectrophotometer (Cary 60 UV-Vis, Agilent, Santa Clara, CA). The prepared RHAMMB-targeting nanocomplexes were stored in DEPC-treated water at 4 °C and used within 2 weeks ...