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Citations for Agilent :
351 - 400 of 501 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: The plasmid encoding NusG-F165A was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit (Stratagene), using vector pET11A_nusG (Burmann et al. ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... amino acids Lys345 Lys346 to Gln 345 Gln 346) was developed by site-directed mutagenesis of the pGEX6p2-Cx43 CT plasmid (Agilent technologies ...
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bioRxiv - Cell Biology 2019Quote: ... The KEN-box mutant was generated in the pMT-Cyclin B1-GFP plasmid using a site-directed mutagenesis kit (Agilent), following the manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... All GFP-Parkin variants were generated using PCR mutagenesis on the GFP-Parkin WT plasmid (addgene#45875) according to the manufacturer’s protocol (Agilent Technologies). Constructs were verified by Sanger sequencing.
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bioRxiv - Bioengineering 2019Quote: Variant CvFAPG462V was generated by site-directed mutagenesis of the wild-type construct in pETM11 using the QuikChange whole plasmid synthesis protocol (Stratagene) with CloneAmp HiFi PCR premix (Clontech) ...
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bioRxiv - Molecular Biology 2019Quote: ... and A110C mutations were introduced into the HHT2 gene of the pRM200 plasmid (CEN4/ARS1/TRP1/HHT2-HHF2) (Mann and Grunstein, 1992) by site directed mutagenesis (QuikChange, Agilent). The WT or mutagenized pRM200 plasmids were then introduced into the RMY102 strain by standard yeast transformation procedures ...
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bioRxiv - Neuroscience 2019Quote: ... the mouse cDNA sequence for Ntrk2 (GenBank accession number X17647, nucleotides 188-722) was amplified by PCR and cloned into pBluscript II KS (-) plasmid (Stratagene). Antisense probes were synthesized with T3 RNA polymerase (Promega #2083 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Biochemistry 2020Quote: E.coli expression plasmids encoding GST-PEX14-NTD with amino acid substitutions were constructed via Site Directed Mutagenesis using QuikChange II (Stratagene). ß-tubulin (amino acids 388–444 ...
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bioRxiv - Biophysics 2021Quote: The triplex-forming regions were incorporated into the parent plasmids by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene) following the manufacturer’s protocol ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The purified PCR products were subsequently used as megaprimers to generate the library of mutants in the plasmid pZE-ProQ-3×FLAG by following GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... were created by point mutations on the pcDNA3.1-Cyb5r3 (WT) plasmid using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200521). Mutagenesis primers were designed using Agilent’s online program ...
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bioRxiv - Microbiology 2020Quote: Huh7.5 cells were seeded 16h prior to transfection with pEGFP-N3-VSV-Gts and p7-encoding plasmid using GeneJammer transfection reagent (Agilent). Medium was changed 4h post-transfection and cells were incubated overnight at 40°C ...
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bioRxiv - Microbiology 2020Quote: ... was generated by introducing mutations in the 3’ splice site of the pPOLI-WSN-M plasmid using QuikChange II site-directed mutagenesis protocol (Agilent). pPolI-WSN-M-M2SMRtoAAA (Mut1) ...
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bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
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bioRxiv - Microbiology 2020Quote: ... ftsL30-121 and ftsW were introduced into some plasmids by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies).
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bioRxiv - Genomics 2021Quote: ... and electroporated in five separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent). After a 1 hr outgrowth in Luria Broth (LB ...
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bioRxiv - Molecular Biology 2021Quote: ... Correspondent seed-sequence mutated Dual-pMIR-report plasmids were obtained using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies). Low-passage COS7 cells were grown in DMEM (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... MinD and ClpX mutant proteins were constructed by site-directed mutagenesis of plasmids containing minD or clpX using the QuickChange II XL Site-directed mutagenesis kit (Agilent) and confirmed by sequencing prior to purification ...
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bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...
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bioRxiv - Microbiology 2022Quote: All of the indicated mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) (66) ...
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bioRxiv - Developmental Biology 2022Quote: ... The pETM11-Sumo3-Tn5E54K,L372P plasmid was obtained from EMBL-Heidelberg and transformed into Escherichia coli BL21 (DE3) Gold cells (Agilent). Bacterial cultures were grown at 30◦C to a density of OD600=0.6-0.8 and protein expression was induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG) ...
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bioRxiv - Neuroscience 2021Quote: ... was constructed by inserting the mKO1 gene (MBL Lifescience, Tokyo, Japan) and the WPRE sequence into an AAV backbone plasmid (pAAV-CMV; Stratagene).
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bioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ...
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bioRxiv - Neuroscience 2021Quote: ... The 481 bp ywhaz amplicon was generated by PCR reaction and cloned into a plasmid using the StrataClone PCR cloning Kit (Agilent). The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... Mutagenesis was carried out to introduce the H840A mutation into eSpCas9(1.1) variant using pJSC114 plasmid (addgene, 101215) and QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518). eCas9 nickase was expressed using the mutagenesis-modified pJSC114 plasmid ...
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bioRxiv - Plant Biology 2022Quote: ... Mutations were inserted into MP and GFP:MP plasmid with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) using primers in Table S5.
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bioRxiv - Cancer Biology 2022Quote: ... V37W) from pLOC-NT5C2 plasmid by site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent Technologies) according to manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2019Quote: ... Catalytically inactive FnCas9 double mutants were generated on pET-His6-FnGFP-TEV-LIC (this paper) plasmid backbone by QuickChange II site directed mutagenesis kit (Agilent) following manufacturer’s protocol with some modifications.
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bioRxiv - Microbiology 2019Quote: ... We used pMotB.com plasmid as the PCR template for these substitutions using a site-directed mutagenesis kit (QuikChange, Stratagene Inc.) yielding plasmids MotB(D24E ...
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bioRxiv - Microbiology 2019Quote: ... site directed mutagenesis on the plasmid-borne cqsR was performed using the QuikChange™ XL Site-Directed Mutagenesis Kit (Agilent) per manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... The product was purified by gel extraction and used as a megaprimer for amplification of the pYES-αSyn-EGFP plasmid by the EZClone method (Agilent). Following DpnI digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... In order to generate the mutant expression plasmids for BMAL2 we used the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (210515, Agilent). The bHLH mutant was generated by converting an arginine to alanine (OaBMAL2_R88A ...
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bioRxiv - Genomics 2021Quote: The different Sen1 HD versions were fused to a C-terminal His8-tag coupled to Vibrio cholerae MARTX toxin cysteine protease domain (61)and expressed from the T7 promoter (see plasmids in table S6) in Escherichia coli strain BL21 (DE3) CodonPlus (Stratagene). Overexpression was induced by growth in auto-inducing medium (62 ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids with mutant YBEY alleles (pcDNA4-YBEY-R55A and pcDNA4-YBEY-H128A) were generated by QuickChange site-directed mutagenesis (Agilent) with the following pairs of primers ...
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bioRxiv - Biochemistry 2021Quote: ... 67)) plasmid as PCR template Bri2 BRICHOS D148N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
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bioRxiv - Biochemistry 2021Quote: ... All reactions were carried out using a TGradient (Biometra) thermocycler and resulting mutant plasmids transformed into electro competent BL21-Gold (DE3) cells (Agilent). The integrity of all constructs and PK mutations were confirmed by sequencing (Genewiz).
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bioRxiv - Biophysics 2020Quote: A Pol I expression vector was generated from the plasmid pXS67 (Yale Coli Genetic Stock Center, Strain CJ803) by site-directed mutagenesis using a QuikChange kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... first an AgeI cut-site was knocked into the pcDNA3.1-Myoferlin-HA plasmid immediately prior to the transmembrane domain by site-directed mutagenesis (Agilent: 210518). Second ...
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bioRxiv - Genomics 2020Quote: BG4-encoding plasmid (kindly provided by Professor Shankar Balasubramanian, University of Cambridge, UK) was transformed into BL21(DE3) competent cells (Stratagene) which were cultured in TY medium (1.6% tryptone peptone ...
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bioRxiv - Microbiology 2021Quote: ... Site-specific mutants in ftsA were constructed by site-directed mutagenesis of ftsA expression plasmids using the QuikChange II XL mutagenesis system (Agilent) and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein mutants were obtained by PCR-based site-directed mutagenesis with the pET-M11-RRM12 (12-235) plasmid as a template according to the QuikChange protocol (Stratagene) and the primers listed in Table S1 ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Genetics 2020Quote: Single variants were introduced by mutagenesis of the Tile 3-KCNH2 plasmid (Table S1) using the Quikchange Lightning Multi kit (Agilent), with 1 primer per variant ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid pAscA was constructed by amplifying the DNA encoding AscA containing PciI and BamHI sticky ends and ligating into plasmid pTrc99a (Stratagene) between the NcoI and BamHI sites ...
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bioRxiv - Biochemistry 2022Quote: Human ALDH9A1 mutants were generated from the template plasmid (EX-Z3075-M29) described above using a QuickChange XL II Site-Directed Mutagenesis Kit (Agilent). Primers were designed with the corresponding mutations as prescribed by the QuickChange Primer Design tool (https://www.agilent.com/store/primerDesignProgram.jsp ...