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Citations for Agilent :
201 - 250 of 501 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
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bioRxiv - Microbiology 2020Quote: ... High concentration plasmid solutions were produced using XL1-Blue Competent Cells (200249, Agilent technology) and plasmid extraction was performed using Qiagen Plasmid Maxikit (12163 ...
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bioRxiv - Cell Biology 2022Quote: ... Amplified cDNA was cloned into pSC plasmid (StrataClone Blunt PCR Cloning Kit, STRATAGENE, 240207). Then ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
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bioRxiv - Physiology 2023Quote: Plasmid constructs used for transient transfection were amplified using XL10-gold ultracompetent cells (Agilent Technologies Inc. ...
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bioRxiv - Biophysics 2023Quote: ... The resultant recombinant plasmids were transformed into ultra-competent ArcticExpress (DE3) cells (Agilent Technologies). ArcticExpress cells are derived from BL21(DE3) ...
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bioRxiv - Biochemistry 2023Quote: ... The point mutation plasmids were generated using QuikChange II kit (Agilent, cat no. 200521) with corresponding primers ...
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bioRxiv - Neuroscience 2023Quote: ... and the constructed AAV-vector plasmid were transfected into AAV-293 cells (#240073, Agilent) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: The bacterial expression plasmid (pk19pA-Apex2-6xHis) was transformed into Bl21DE3 Gold (Stratagene# 230132) according to manufacturer instruction ...
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bioRxiv - Biochemistry 2023Quote: ... each plasmid was transformed into BL21-CodonPlus(DE3)-RIPL competent cells (Agilent Technologies, #230280). A single colony was picked and inoculated in 1 mL of terrific broth (TB ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression plasmids were transformed into Escherichia Coli strain BL21-Codonplus(DE3)-RIL (Stratagene). The expression and purification of each protein was performed as described previously.77 Briefly ...
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bioRxiv - Biochemistry 2024Quote: The codon-optimized plasmid for hPLIN1 was expressed in Escherichia coli ArcticExpress (DE3) (Agilent) while codon optimized plasmids hPLIN2 and hPLIN3 were expressed in E ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Cancer Biology 2021Quote: ... The other plasmids were obtained by PCR using the QuickChange Site-Directed Mutagenesis Kit (Stratagene) using specific oligonucleotides as follows ...
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bioRxiv - Genetics 2021Quote: ... SCN5A individual zones on small plasmids were mutagenized using the QuikChange Lightning Multi kit (Agilent) with primers designed using the online QuikChange Primer Design tool ...
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bioRxiv - Genetics 2021Quote: ... while the longer “plasmid” PCR amplicons were generated using Herculase II Fusion DNA Polymerase (Agilent). Primer sequences used for chimeragenesis are listed in Table S8.
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bioRxiv - Neuroscience 2022Quote: ... the plasmid was amplified using chemically competent cells deficient for recombinase activity (Agilent Technologies #200152), purified (Macherey-Nagel #740416.10) ...
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bioRxiv - Cell Biology 2019Quote: The pHMT-KTN1 plasmid was transformed into BL21-CodonPlus (DE3)-RIPL competent cells (Agilent Technologies). Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210) ...
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bioRxiv - Cell Biology 2021Quote: ... Modifications of these plasmids were made using the QuikChange Lightning site-directed mutagenesis kit (Agilent). The C-termini were tagged with mGFP or mCherry to facilitate protein localization ...
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bioRxiv - Microbiology 2021Quote: ... we also assessed the quality of our plasmid DNA preparations using a 2100 Bioanalyzer (Agilent).
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bioRxiv - Molecular Biology 2020Quote: ... p53/pET28a plasmid was then templated in Quickchange Lightning Site-Directed mutagenesis kit (Agilent Technologies) to create the desired mutations in p53-(1-73 ...
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bioRxiv - Biochemistry 2022Quote: ... Point mutants were introduced using whole plasmid amplification with Pfu Ultra II (Agilent, 600670-61) and complementary primers ...
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bioRxiv - Biochemistry 2022Quote: ... and cloned into a plasmid vector using the StrataClone blunt PCR cloning kit (Agilent; 240207). Colonies were selected and grown in 4ml 2xTY media supplemented with Ampicillin (10μg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR products were cloned into a pCMV-Tag2 plasmid (Stratagene, San Diego, CA, USA) digested with BamHI and SalI containing FLAG-tag and S-tag sequences ...
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bioRxiv - Plant Biology 2019Quote: ... the pET21 plasmid containing PL1 was transformed into BL21 DE3 pLyS cells (Agilent, Edinburgh, UK). PL1 expression was induced at mid-log phase by supplementing the media with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Cell Biology 2019Quote: ... K-R mutant plasmids were generated using Quikchange II XL site-directed mutagenesis kit (Agilent), with primers listed in Table S5 ...
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bioRxiv - Biochemistry 2021Quote: ... The pEYKBA plasmid was mutagenized by site-directed mutagenesis using the QuikChange II kit (Agilent) to create point mutations in the kinase domain of Bcr-Abl ...
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bioRxiv - Microbiology 2021Quote: All transformations for cloning or plasmid amplification were performed in Escherichia coli XL10 Gold (Agilent) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Adenoviral plasmid was linearized by PacI digest and transfected into AD-293 cells (Agilent Technologies) to produce recombinant viral particles ...
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bioRxiv - Molecular Biology 2022Quote: ... Various point mutant plasmids were prepared using QuikChange II XL site-directed mutagenesis kit (Agilent). QIAprep spin miniprep kit was utilized to make recombinant plasmids following vendor’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... We purchased the pISRE-Luc and pNFκB-Luc plasmids (Stratagene Products Division, Agilent Technologies, Inc.). To create pIFNB1-Luc ...
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bioRxiv - Cancer Biology 2023Quote: ... was used to generate expression plasmids with FH variants using a QuikChange II kit (Agilent). Sanger sequencing was used to confirm sequence identity.
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bioRxiv - Cell Biology 2023Quote: ... The same plasmid was subjected to site-directed mutagenesis using QuikChange Lightning kit (Agilent Technologies) to generate the TagRFP-tagged constitutively active odr-3 mutant (ODR-3Q206L).
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bioRxiv - Microbiology 2023Quote: ... expression plasmids described above were transformed into BL21(DE3) or BL21(DE3)-RIL cells (Agilent), plated on MDG media plates (1.5% Bacto agar ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid pEGFP-Dynamin 2.WT was constructed using a Quik Change II XL (Agilent) site-directed mutagenesis kit to change the GCC (alanine ...
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bioRxiv - Cell Biology 2024Quote: ... Site-directed mutations in the plasmids were generated using the QuikChange mutagenesis protocol (Agilent Technologies). The probes (full length of EGFP ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µg of plasmid ASAP3 and ASAP3-R3 were added into the solution of GeneJammer (Agilent) transfection reagent in Opti-MEM (3 µL in 200 µL) ...
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bioRxiv - Immunology 2021Quote: Spike-expressing plasmid constructs were generated using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent) on a previously described Wuhan-hu-1 template34 ...
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bioRxiv - Microbiology 2021Quote: ... Mutations were introduced into the pMicC plasmid using a QuikChange Lightning site-directed mutagenesis kit (Stratagene). Oligonucleotides used in this study are listed in Table S2.
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bioRxiv - Plant Biology 2021Quote: ... Plasmids used for rice transformation were created by site-directed mutagenesis (Quikchange lightning technology, Agilent technologies) to introduce point mutations in the genomic sequence of RGA5 already cloned in pAHC17 ...
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bioRxiv - Immunology 2021Quote: ... ORF9 mutant plasmids were generated using site directed mutagenesis (QuikChange II Site-Directed Mutagenesis Kit, Agilent). GST-fusion bacterial expression vectors for FLAG-ORF9 ...
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bioRxiv - Molecular Biology 2022Quote: ... Longer inserts flanked by longer homology arms were cloned into pBluescript II KS-plasmid (Agilent Technologies). All TALEN and Cas9 target sequences and donor sequences for homologous recombination are listed in Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... AAV vectors were generated by triple transfection of 293T/17 cell line using Polyethylenimine (PEI) and plasmids pAAV-hsynapsin-HA-ΔN-DGKκ or pENN.AAV.hSynapsin.EGFP.RBG together with pHelper (Agilent) and pAAV2/9 or pAAV2/Rh10 (provided by J.Wilson and J.Johnston at Penn Vector Core) ...
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bioRxiv - Cell Biology 2020Quote: ... All mutations in the plasmids were generated by site-directed mutagenesis using a QuikChangeII kit (Agilent) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... All mutations in the plasmids were generated by site-directed mutagenesis using a QuikChangeII kit (Agilent) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: All AAV6 vectors were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), which contains inverted terminal repeats (ITRs ...
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bioRxiv - Cell Biology 2021Quote: ... The pEGFP-C2-Myo15-2(jd) plasmid was generated using site directed mutagenesis (QuikChange II, Agilent) to introduce the jordan (c.4940A>G ...
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bioRxiv - Neuroscience 2022Quote: ... together with 40 ng/μl of a transfection reporter plasmid encoding humanized Renilla GFP (hrGFPII; Stratagene) and 0.01% Fast Green (AppliChem).