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Citations for Agilent :
151 - 200 of 5950 citations for Rat Mesoderm Specific Transcript Homolog Protein MEST ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... α-FLAG MAb (rat) (M2) was purchased from Agilent (Santa Clara, CA). α-HA MAb (mouse) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with rat anti-mouse Ki67 (1:50, DAKO #M7249) for 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... for 1.5h at RT and HRP-conjugated rabbit anti-rat (Agilent, P0450) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The non-specific binding of antibodies was blocked using 10% Normal Goat Serum (DAKO, Glostrup, Denmark) in PBS for 20 minutes at RT ...
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bioRxiv - Biochemistry 2021Quote: ... followed by first strand cDNA synthesis primed with gene-specific oligonucleotides using high-fidelity Accuscript (Agilent). DNA fragments were amplified in two rounds of PCR as previously described (46 ...
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bioRxiv - Microbiology 2020Quote: ... annealed with gene-specific primer and reverse-transcribed using AffinityScript Multiple Temperature Reverse Transcriptase (#600107, Agilent) with following conditions ...
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bioRxiv - Microbiology 2020Quote: ... annealed with gene-specific primer and reverse-transcribed using AffinityScript Multiple Temperature Reverse Transcriptase (#600107, Agilent) with following conditions ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared and barcoded with the SureSelect Strand Specific RNA Library Prep system (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Compound-specific carbon stable isotope compositions of sterols were measured on a gas chromatograph (Agilent 6890) coupled with a Thermo Finnigan Combustion III interface to a Finnigan Delta Plus XL isotope ratio mass spectrometer (GC-IRMS) ...
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bioRxiv - Cancer Biology 2021Quote: ... and specific primers (Table 1) were performed using an AriaMX Real-time PCR system (Agilent Technologies). Relative expression of mRNAs was determined after normalization against housekeeping genes TBP ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... protein block (Dako) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Primary rat neurons or human i3Neurons were plated on Seahorse XFp plates (Agilent) at a density of 40,000 cells/well ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were then stained with polyclonal rabbit anti-rat IgG (1:200, Dako), or EnVision rabbit (Dako ...
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bioRxiv - Microbiology 2021Quote: ... A rat anti-FLAG monoclonal antibody (200474) was purchased from Agilent (Santa Clara, CA). MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I ...
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bioRxiv - Neuroscience 2023Quote: ... Primary α-DAT was detected by using secondary rabbit anti-rat (1;1,000; Dako, P0450). Upon visualization by using the ECL Advanced Chemiluminescence kit (GE Healthcare Life Sciences® ...
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 was detected using a rat an,-Ki67 monoclonal an,body (Dako M7249) at a 1:25 dilu,on followed by bio,nylated goat an,-rat an,body secondary (Jackson ImmunoResearch 112-065-167 ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Neuroscience 2020Quote: Site-directed insertions were made in GluN1 (GluN1-a) (NCBI Protein database accession no. P35439) or GluN2A (Q00959) subunits with the QuikChange site-directed mutagenesis kit (Agilent) with XL1-Blue super-competent cells ...
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bioRxiv - Microbiology 2020Quote: ... A construct expressing the MBP-MogRQN→AA protein was created by site-directed mutagenesis of pMAL-p5x-mogR using the QuikChange II Site-Directed Mutagenesis Kit (Stratagene) with the same primers as for pHT304-Pxyl-MogRQN→AA ...
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bioRxiv - Cell Biology 2022Quote: ... sor1-D303A and sor1-Y305A variants proteins) were introduced into the cloned sor1 gene using the QuikChangeII kit (Agilent Technologies) and inserted into the genome by transformation.
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bioRxiv - Cell Biology 2021Quote: ... The mutants were created by the same method for site-directed mutagenesis using specific primers designed from Agilent QuickChange Primer design web tool ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand specific RNA sequencing was performed on quality controlled high RIN value (>7) RNA samples (Bioanalyzer Agilent Technologies). In brief ...
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bioRxiv - Synthetic Biology 2022Quote: Specific biosensors identified during the selection and subsequent CleaveSeq analysis were resynthesized on an oligonucleotide array (Agilent, G7220A). The array contained 1,730 sequences each padded to a length of 158 nt ...
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bioRxiv - Immunology 2023Quote: ... The LCMV-specific antigen tetramer was prepared by conjugating the biotinylated LCMV-NP to SA-PE (Prozyme PJRS25) at a concentration ratio of 4:1 respectively ...
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bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were prepared according to the instruction of the Agilent protein 80 kit and analyzed on a 2100 Bio-analyzer device (Agilent technologies). The ATPase activity of FSS-PscN was determined by measuring the release of total phosphate at 37°C for 30 minutes which was detected by the malachite green method (Casabona et al. ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) followed by Protein block (X090930-2, Agilent Technologies Inc., Santa Clara, CA). Primary antibodies were applied at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... was performed by LC/MS/MS by an MRM approach using the specific transition of cGMP (m/z 346.0 → 152.0) (1200 HPLC system (Agilent Technologies) coupled to an LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... PLK1 mutant plasmids were generated using specific primers (sequences available upon request) following the manufacturer’s instructions (QuickChange II, Stratagene). shPLK1 UTR ...
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bioRxiv - Microbiology 2022Quote: ... Specific antigen-antibody reactions were visualized by means of 3,3’-diaminobenzidine tetrahydrochloride staining using the Dako Envision system (Dako Cytomation ...
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bioRxiv - Molecular Biology 2022Quote: ... Extracted ion chromatograms of the compound-specific mass transitions were integrated using MassHunter software (Agilent, Santa Clara, CA, USA). Absolute concentrations were calculated based on an external calibration curve.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the assay medium was prepared by supplementing Seahorse XF Base medium (pH 7.4) with a specific combination of 10 mM glucose (100X stock, Agilent), 1 mM pyruvate (100X stock ...
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bioRxiv - Pathology 2023Quote: ... Specific antibody binding was detected using a second antibody and the LSAB system (Dako Corporation, Carpinteria CA, USA, K690) for 30 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific process was as follows: Chromatographic elution was performed on an Agilent 1290 infinity system (Agilent technologies, USA) equipped with a sample manager coupled to a mass spectrometry with an electrospray ion source in both positive and negative ion modes ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Cancer Biology 2020Quote: ... dry protein A cartridges (Agilent) were primed in PBS at 300 μL/min before loading 1 mg of antibody at 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Protein block (DAKO) for 10min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... BSA and Protein block (Dako). Sections were stained with primary antibodies against GFP (ab290 ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Molecular Biology 2019Quote: ... The original eGFP-C2-DEF6 plasmid (5) was used to generate the mutant DEF6 proteins by mutagenesis PCR (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent Technologies, #210516). All mutations were verified by sequence analysis (see Suppl ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...