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Citations for Agilent :
301 - 350 of 500 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The 5’mitoUTR was cloned into the TFAM-mScarlet/pWPXL or the 3’mitoUTR_TFAM-mScarlet_pWPXL plasmid using megaprimers following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... was used as a template for generating expression plasmids with FH variants using the QuikChange II kit (Agilent). Sanger sequencing was used to confirm sequence identity for all plasmids ...
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bioRxiv - Genomics 2024Quote: ... the pETM11-Sumo3-Tn5(E54K,L372P) plasmid was transformed into Escherichia coli BL21(DE3) codon + RIL cells (Stratagene). Cells were grown in TB-FB media (1.2 % Bacto-Tryptone ...
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bioRxiv - Biophysics 2024Quote: The WT-CtNAT10-pFastBacHT A plasmid was used to prepare all CtNAT10 mutants using the QuikChange protocol (Stratagene) 53 ...
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bioRxiv - Biophysics 2024Quote: ... All GαS variants were obtained from this plasmid by site-directed mutagenesis using the QuikChange II kit (Agilent) and primers listed in Table S2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression plasmids were constructed with the obtained PCR product using the SureVector system (Agilent, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... we introduced the corresponding single nucleotide mutation in the 3xFLAG donor plasmid using QuikChange Site-Directed Mutagenesis Kit (Agilent). Then this donor plasmid was used for the same process used to generate the BAG3WT-FLAG line25 ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid point mutations were generated using Pfu Ultra polymerase by Quick Change PCR mutagenesis (Agilent Technologies, Santa Clara, CA). The resulting DNA constructs and strains were verified by DNA sequencing.
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bioRxiv - Microbiology 2020Quote: ... Unit genome-length fragments (approximately 2,600 nucleotides) were excised and ligated into the pBLUESCRIPT-KS+ (pKS+) plasmid vector (Stratagene), previously cleaved with the same enzyme ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid with smc3-K112,113R (AMp1392) was generated by site-directed mutagenesis (using QuikChange XL Site-directed mutagenesis kit, Agilent). Both plasmids were integrated into the LEU2 locus in the pCLB2-3HA-SMC3 strain background.
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bioRxiv - Immunology 2022Quote: ... Amber (TAG) codons were introduced site-specifically in the 2B4 TCR and CD3 plasmid using Quikchange mutagenesis kit (Agilent).
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bioRxiv - Microbiology 2022Quote: Single-base alterations to plasmid sequences were performed using a PCR technique based on QuikChange site-directed mutagenesis (Stratagene).
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bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis of the human yb-1 coding gene was carried out directly on the pET22b-YB-1_1-180 expression plasmid by using the “Quikchange II XL site-directed mutagenesis kit” from Stratagene and appropriate oligonucleotides (Eurofins Genomics) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we deleted two existing Bsa-I sites from the plasmid by site-directed mutagenesis (Agilent, Santa Clara, CA, #200521). In the clonal strain production ...
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bioRxiv - Microbiology 2021Quote: ... Cassettes and the template plasmid pM07704 were amplified by polymerase chain reaction (PCR) with Herculase II DNA polymerase (Stratagene). Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline ...
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bioRxiv - Microbiology 2021Quote: ... Storz and pRprAmut2 was constructed by amplification of the pRprA plasmid with mutagenic primers using the Pfu enzyme (Agilent), followed by DpnI digestion and transformation into the cloning strain NEB5-alpha F’Iq ...
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bioRxiv - Microbiology 2019Quote: ... and pGal4-UAS-Luc plasmids (0.3 µg/well; provided by Dr. Yves Jacob) or IFN-β-pGL3 (0.3 µg/well; Stratagene) together with pRL-CMV reference plasmid (0.03 µg/well ...
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bioRxiv - Immunology 2019Quote: ... the parental Env-encoding plasmid was altered by site-directed mutagenesis using the QuikChange site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... The region containing random fragments of the VWF plasmid was made double stranded using primers P9/P10 (annealing temperature = 65.0°C) and Herculase II (Agilent) with 16-20 rounds of PCR amplification (Figure S1B) ...
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bioRxiv - Genomics 2021Quote: ... coli were generated by introducing the corresponding mutations in plasmid pDL893 using the QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Biochemistry 2021Quote: Individual point mutations were introduced into AsCap5 plasmid by Quick-change mutagenesis according to the direction provided by Agilent. Oligonucleotides employed for creating mutants were ordered from IDT ...
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bioRxiv - Neuroscience 2021Quote: ... Fusion products were digested with NotI and ligated to a NotI cleaved pAAV-MCS plasmid backbone (#240071-5, Stratagene). The final rAAV/SORL1 KO-Neo plasmid construct was verified by NotI digestions and sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, Flag-NLS-linker-Cas9 (Baker et al., 2016) was co-electroporated with a pAAV backbone-based plasmid (Stratagene) containing two U6-promotor-driven gRNAs targeting HPRT (GACTGTAAGTGAATTACTT) ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent), 0.2 μM oligonucleotide encoding the change ...
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bioRxiv - Microbiology 2022Quote: ... the vector pEC1_ptpA previously constructed [22] was used as template to generate the suicide plasmid pEC1_ptpA_D120A derivative by using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies) with the primer #1559 (5’-GGAAGAGAGTGATGTACCAGCTCCATACTACACGAATAATT-3’) ...
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bioRxiv - Cancer Biology 2022Quote: ... PLK1 mutant plasmids were generated using specific primers (sequences available upon request) following the manufacturer’s instructions (QuickChange II, Stratagene). shPLK1 UTR ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Molecular Biology 2023Quote: ... megaprimers were synthesized and cloned into the TFAM-mScarlet_pWPXL plasmid following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... The pHD1344tub(PAC)-Nterm3V5-A3 plasmid was mutagenized using the QuickChange Lightning site-directed mutagenesis kit (Agilent, product #210518) and primers listed in Table S2 ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Neuroscience 2023Quote: ... nucleotides 214–990) was amplified by PCR and cloned into the pBluscript II KS (−) plasmid (Stratagene, La Jolla, CA). Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... COS1 cells were transfected with pAdTrack-CMV expression plasmids (Table S1) using the GeneJammer transfection reagent (#204130, Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The PAM sequences within each donor plasmid were mutated using site directed mutagenesis with Pfu Turbo (Agilent, Cat# 600250) to avoid targeting of the donor plasmid by Cas9 nuclease ...
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... a codon-optimized gene block was cloned into the 6xHis_1B LIC plasmid (gift from Scott Gradia, UC Berkeley) and transformed into Arctic Express bacteria (Agilent). Expression was induced with 0.3 mM IPTG for 16 hr at 20°C ...
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bioRxiv - Neuroscience 2023Quote: AAV plasmids carrying cDNA for hIL-6 downstream of a CMV promoter were co-transfected with pAAV-RC (Stratagene) encoding the AAV genes rep and cap ...
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bioRxiv - Molecular Biology 2023Quote: ... The reference plasmids containing PA tag sequence were constructed in pBluescript II SK (+) vector (Agilent, Palo Alto, CA, USA). One μg of guide RNA inserted vector and 1.0 μg of reference plasmid were co-transfected into EGRG01 embryonic stem (ES ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Genetics 2024Quote: ... the E171Q variant was introduced into an SCN5A expression plasmid (pCDNA3.1) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent). HEK293T cells were grown at pH 7.4 in filtered sterile DMEM nutrient medium (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids for His6-ZZ-TEV-MZT2 and GST-GCP2-NHD were co-transformed into BL21-CodonPlus (DE3)-RIL (Stratagene) cells ...
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bioRxiv - Microbiology 2024Quote: ... R80A and H71R) or pLRGatewayIRESeYFP-Vpr plasmids were synthesised using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions and primers listed in Table 2 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids coding for hSSB1 variants were generated from the Y85C substitution-corrected pET29a-hSSB1 vector using the QuikChange (Agilent) mutagenesis kit ...
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... variant hLPYK proteins were created by mutating the coding region of the pLC11 plasmid (originally a kind gift from Dr. Andrea Mattevi 28) using QuikChange (Stratagene) and primers designed to individually obtain all 19 substitutions at each position ...
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bioRxiv - Biochemistry 2022Quote: ... The various mutant forms of this plasmid were made with the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent using the appropriate primers each time (Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid encoding NusG-F165A was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit (Stratagene), using vector pET11A_nusG (Burmann et al. ...