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Citations for Agilent :
101 - 150 of 500 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Human PKM2 wild-type construct was purchased from Addgene (44242) and sub-cloned to pCMV-Tag2B plasmid (Agilent Technologies). PKM2 S437Y mutant was generated by site-directed mutagenesis and sub-cloned to the same plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... All fragments were sub-cloned into the pUC19 plasmid (Stratagene) using restriction enzymes ...
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bioRxiv - Plant Biology 2020Quote: ... and then cloned into the pBC SK+ plasmid vector (Stratagene). The resulting construct was used to produce the translation initiation codon mutations by PCR ...
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bioRxiv - Genetics 2022Quote: ... All plasmid samples were isolated from the SURE strain (Stratagene) by the plasmid maxi-kit (Qiagen Inc.)
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bioRxiv - Microbiology 2022Quote: ... Flanks were then ligated into a pBSK bluescript plasmid (Stratagene) and 50 μg of DNA was co-transfected into 3D7 WT (FP2a ...
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bioRxiv - Microbiology 2022Quote: ... We purchased the pISRE-Luc and pNFκB-Luc plasmids (Stratagene Products Division ...
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bioRxiv - Cell Biology 2023Quote: Plasmid constructs were transformed into Escherichia coli BL21 DE3 (Stratagene) stains ...
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bioRxiv - Microbiology 2023Quote: ... Expression plasmids were transformed into BL21 (DE3) RIL cells (Agilent) and plated onto MDG media (1.5% Bacto agar ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into BL21-CodonPlus (DE3)-RIL (Stratagene) cells and expressed in 12 L of LB after induction with 0.5 mM IPTG at 18 °C for 16 hr ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was transformed into E.coli BL21(DE3) cells (StrataGene) and protein expression induced with 0.5 mM IPTG at 37°C for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 36 ng/well of pEGSH-LUC luciferase reporter plasmid (Agilent Technologies), and 0.9 ng/well of pRL-CMV Renilla luciferase reporter plasmid (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... along with 60 ng/well of pERV3 receptor plasmid (Agilent Technologies) containing a modified ecdysone receptor (VgEcR ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pET42a(+)-pbp2E114Q was constructed using QuikChange site-directed mutagenesis (StrataGene) with primers YQ1 and YQ2 and plasmid template pET42a(+)-pbp2.
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670.) pcDNA Plk4(Sak ...
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bioRxiv - Biochemistry 2020Quote: ... The mDHFR plasmid was transformed into BL21-Gold(DE3) (Agilent Technologies) host cells ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were then transformed into BL21-Gold (DE3) cells (Agilent Technologies). Cells were grown overnight in 5 mL of LB medium with 25 µgmL-1 kanamycin and used to seed 500 mL cultures for growth at 37°C ...
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bioRxiv - Genetics 2022Quote: ... All plasmid constructs were isolated from the E.coli SURE strain (Stratagene) and (GAA)100 repeats were confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: ... The PCR amplicon was cloned in the pGEMT-easy plasmid (Stratagene) for further use ...
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bioRxiv - Neuroscience 2022Quote: ... and subcloned into the pAAV plasmid (AAV Helper-Free System, Agilent). A plasmid map for the scIgG-306C7B3 expression construct is shown in the Supplementary Materials ...
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bioRxiv - Immunology 2023Quote: Antibody heavy chain plasmids were mutated using the QuikChange Lightning (Agilent) kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression plasmid was transformed into BL21-CodonPlus Competent Cells (Agilent), and protein expression was done by the addition of IPTG (1 mM final concentration ...
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bioRxiv - Biochemistry 2023Quote: ... for plasmid amplification or BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for protein expression in bacteria ...
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bioRxiv - Biochemistry 2023Quote: ... All recombinant plasmids were transformed into XL1-Blue competent cells (Agilent) and sequenced for verification.
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bioRxiv - Microbiology 2023Quote: ... The plasmids were transformed into BL21-codon plus RIL cells (Agilent) and were plated onto an LB agar plate with ampicillin (100 μg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... and cloned plasmids were transformed into XL-1 Blue cells (Agilent). Transformed cells were plated on Luria–Bertani (LB ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibodies used were goat anti-rabbit and rat anti-rabbit IgG-HRP (Dako, Santa Clara, CA, USA). Proteins were detected by chemiluminescence using the Clarity™ Western ECL Substrate coupled to a ChemiDoc XRS+(Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid for overexpression of the M495L mutant was prepared by Quickchange (Stratagene) mutagenesis of the pET28b-MtbRho plasmid encoding WT MtbRho (kindly provided by Dr ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cloned into pSC-A-amp/kan Strataclone plasmids (Agilent) to generate probes for wholemount in situ hybridization experiments (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... The resulting plasmid was transformed into XL10 gold competent E.coli cells (Agilent). Plasmids for transfection were prepared by Midi-preps (QIAGEN ...
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bioRxiv - Molecular Biology 2019Quote: Plasmids were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent #NC9122855), and single colonies were grown as overnight cultures in 5 mL terrific broth (TB ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids pET21b containing corresponding genes were transformed into BL21 (DE3) Gold (Agilent). For GyrA ...
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bioRxiv - Genetics 2020Quote: ... The plasmid was subcloned into pIRES-1a-hrGFP (Stratagene; San Diego, CA) plasmid ...
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bioRxiv - Microbiology 2021Quote: ... High concentration plasmid solutions were produced using XL1-Blue Competent Cells (Agilent) and plasmid extraction was performed using Qiagen Plasmid Maxikit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... The plasmids were transformed into Escherichia coli strain BL21 (DE3) RIL (Stratagene). Expression was induced at 16 °C overnight with 0.2 mM of IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... the pFR–Luc and pFA2– cJun plasmids from Stratagene (now, Agilent Technologies), and the pRL–SV40 plasmid from Promega ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... This plasmid was mutated with a Quikchange Lightning Multi-site kit (Agilent) to create C282Y ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutant plasmids for yeast promoter analyses were constructed by Quikchange mutagenesis (Stratagene) following adaptation for use of Phusion DNA polymerase (NEB ...
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Identification of a motif in Trm732 required for 2’-O-methylation of the tRNA anticodon loop by Trm7bioRxiv - Genetics 2021Quote: ... Plasmids expressing Trm732 and THADA variants were generated by quickchange PCR (Stratagene) or Q5 site-directed mutagenesis (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR products were cloned into pCMV-3XFlag plasmid (Agilent Technologies, USA). To generate a GFP-LC3-RFP autophagic flux reporter construct ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified DNA was cloned into the GST-pGEX-2TK plasmid (Stratagene). GST-FHA fragment was expressed into E.coli BL21PlysS at 30°C for 4h ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting plasmids were inserted into XL10-Gold ultracompetent cells (#200314, Agilent) via heat shock as indicated by the provider and extracted with standard Miniprep (#K0503 ...
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bioRxiv - Biophysics 2024Quote: Each GαS plasmid was transformed into BL21-CodonPlus (DE3)-RIL cells (Agilent) and the bacteria were grown at 30 °C in M9 medium (50 mM Na2HPO4 ...
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bioRxiv - Physiology 2023Quote: ... The DNA plasmids were treated with Dpn I restriction enzyme (Agilent Technologies) to digest the methylated parental DNA template and subsequently transformed into XL10-Gold competent cells (Agilent Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and incubated with biotinylated rabbit anti-rat IgG for 1h (1:200, DAKO Cytomation A/S, Denmark). Following avidin-biotin-peroxidase complex (Vector laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... After washing with TBST three times, membranes were exposed to appropriate secondary antibodies (anti-rabbit, anti-mouse, and anti-rat) coupled to HRP (Dako) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed and incubated with the corresponding secondary antibodies when needed (rabbit anti mouse, Abcam; rabbit anti rat, Vector; Goat anti-rabbit, Dako) and visualization systems (Bond Polymer Refine Detection ...
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bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
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bioRxiv - Cancer Biology 2023Quote: Dual indexed whole exome capture libraries were prepared from mouse and naked mole-rat skin biopsies and matched livers using SureSelect XT HS and XT Low Input Enzymatic Fragmentation protocol (Agilent). For mouse whole exome sequencing (WES ...
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bioRxiv - Immunology 2024Quote: ... In Nr4a1-/- and Nr4a1+/+ mouse thrombus sections neutrophils were visualized by anti-Ly6G (clone: 1A8; isotype: rat IgG2a) and MPO (rabbit polyclonal, #GA51161-2, DAKO; isotype: rabbit immunoglobulin fraction, DAKO). Alexa labeled secondary antibodies (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... In Nr4a1-/- and Nr4a1+/+ mouse thrombus sections neutrophils were visualized by anti-Ly6G (clone: 1A8; isotype: rat IgG2a) and MPO (rabbit polyclonal, #GA51161-2, DAKO; isotype ...