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Citations for Agilent :
351 - 400 of 954 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: OCR in human liver cells was measured using XFp Extracellular Flux Analyzers (Agilent Seahorse Biosciences). The cells were plated into XFp cell culture mini plates for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: Libraries were generated using the Agilent SureSelect Human All Exon V6 kit (Agilent Technologies, USA) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... cDNA for Sp110 CARD (aa 6-110) were amplified from MegaMan Human Transcriptome Library (Stratagene). cDNA for the tandem SUMO interaction motifs of RNF4 (aa 38-129 ...
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bioRxiv - Immunology 2019Quote: ... and used as hybridization probe on Whole Human Genome Microarrays (Agilent Technologies, Palo Alto, CA). Only probes with signal values >60% quantile in at least one condition were considered for the differential gene expression (DGE ...
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bioRxiv - Neuroscience 2021Quote: ... CD20 (monoclonal mouse – anti-human CD20 IgG2a, clone L26, cat. no. M075501-2, Agilent Technologies), 1:800 ...
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bioRxiv - Immunology 2021Quote: ... beads were incubated with 5 μg/ml FITC-conjugated rabbit anti-human C1q (Dako, F0254).
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bioRxiv - Cancer Biology 2021Quote: ... and enriched with the human kinome DNA capture baits (Agilent Technologies, Santa Clara, CA, USA). Six libraries were pooled for each capture reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 ng of genomic DNA from the test samples and human reference DNA (Agilent Technologies) were differentially labeled with Cy5-dCTP and Cy3-dCTP by random priming ...
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bioRxiv - Biochemistry 2023Quote: ... genomic DNA libraries were prepared using the SureSelect Human All Exon V6 kit (Agilent Technologies). Exome libraries were subjected to next generation sequencing using DNBseq platform (MGI ...
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bioRxiv - Cancer Biology 2023Quote: ... and the SureSelect XT HS Human All Exon V7 target-enrichment probes (5191-4028, Agilent) for exome capture ...
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bioRxiv - Genetics 2023Quote: ... exome enrichment was performed using the SureSelect Human All Exon 50 Mb Kit V5 (Agilent). Sequencing was done on a HiSeq4000 (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... Immunodetection of TTR monomers was performed by employing rabbit anti-human TTR polyclonal antibody (Dako) as a primary and goat anti-rabbit antibody labelled with DyLight 680 (SERACARE ...
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bioRxiv - Immunology 2024Quote: ... WES libraries were prepared using the SureSelect Human All Exon V8 Kit (Agilent; 5191-6873) and sequenced on either a NextSeq 2000 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then co-transfected with two helper plasmids (pHelper and pAAV-RC; Stratagene) and one of the pAAV plasmids (pAAV-NT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The control plasmid was pCMV-3Tag-3A (pCMV) (Agilent Technologies, Santa Clara, CA, U.S.A.). HEK293 cells were seeded at a density of 1 × 104 cells per well in either 96- or 24-well plates and transfected 24 hours later using JetPRIME (Polyplus Transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... template WT plasmid was digested by the addition of 1 μL DpnI (Agilent Technologies) for 5 min at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were propagated in Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, CA), after growth in Lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2019Quote: ... All sequence changes to the plasmid were accomplished by site directed mutagenesis (QuikChange, Agilent). The histone H3 plasmid from this reference contains an H3-G102A mutation ...
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bioRxiv - Microbiology 2021Quote: ... The pShuttle-ACE2 plasmids were then electroporated 440 into BJ5183 AD-1 cells (Agilent), which were pretransformed with pAdEasy-1 to facilitate recombination with the pShuttle-CMV vector ...
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bioRxiv - Biochemistry 2021Quote: ... Sequence-verified plasmids were transformed into ArcticExpress (DE3) competent cells (Agilent Technologies, California, USA) for protein expression ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then co-transfected with two helper plasmids (pHelper and pAAV-RC; Stratagene) and one of the pAAV plasmids (pAAV-DCUg-NT ...
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bioRxiv - Cell Biology 2021Quote: ... This plasmid was used as template for site-directed mutagenesis (QuickChange kit, Agilent Technologies) with the following oligonucleotides:
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bioRxiv - Systems Biology 2021Quote: ... For plasmid propagation chemically competent Escherichia coli XL1-Blue (Agilent Technologies, Santa Clara, CA) cells were used which were grown in lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was transformed into Escherichia coli strain BL21-Codon-plus(DE3)-RIL (Stratagene). Bacteria was grown in LB broth in a shaker at 37°C until reaching the log phase (A600nm between 0.4 and 0.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The GYG1p.Y195F mutant was generated from this plasmid using the QuickChange mutagenesis kit (Stratagene). Codon optimised genes for GYS1 and aa 264/294-350 GYG1 (GYG1ΔCD ...
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bioRxiv - Biophysics 2022Quote: ... Channel mutations were introduced into this plasmid using the QuickChange Lightning kit from Agilent. All portions of the resulting construct that were subject to PCR were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2021Quote: ... Mutated plasmids were generated by Quick-Change Site-directed Mutagenesis (Table 1) (Agilent Technology) based on the secondary structure model of the 5’UTR of A3G mRNA (40 ...
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bioRxiv - Biochemistry 2021Quote: pGEX4T-3 expression plasmids were transformed into BL21-Codon Plus RIPL competent cells (Agilent). Protein expression was induced at 20°C for 3.5 hr ...
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bioRxiv - Synthetic Biology 2020Quote: Mutagenesis was performed on the pET21b(+)-TrpR-Wt plasmid using the QuikChange technique (Stratagene) following the manufacturer’s protocol.
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bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
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bioRxiv - Microbiology 2020Quote: ... High concentration plasmid solutions were produced using XL1-Blue Competent Cells (200249, Agilent technology) and plasmid extraction was performed using Qiagen Plasmid Maxikit (12163 ...
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bioRxiv - Cell Biology 2022Quote: ... Amplified cDNA was cloned into pSC plasmid (StrataClone Blunt PCR Cloning Kit, STRATAGENE, 240207). Then ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
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bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
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bioRxiv - Physiology 2023Quote: Plasmid constructs used for transient transfection were amplified using XL10-gold ultracompetent cells (Agilent Technologies Inc. ...
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bioRxiv - Biophysics 2023Quote: ... The resultant recombinant plasmids were transformed into ultra-competent ArcticExpress (DE3) cells (Agilent Technologies). ArcticExpress cells are derived from BL21(DE3) ...
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bioRxiv - Biochemistry 2023Quote: ... The point mutation plasmids were generated using QuikChange II kit (Agilent, cat no. 200521) with corresponding primers ...
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bioRxiv - Neuroscience 2023Quote: ... and the constructed AAV-vector plasmid were transfected into AAV-293 cells (#240073, Agilent) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: The bacterial expression plasmid (pk19pA-Apex2-6xHis) was transformed into Bl21DE3 Gold (Stratagene# 230132) according to manufacturer instruction ...
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bioRxiv - Biochemistry 2023Quote: ... each plasmid was transformed into BL21-CodonPlus(DE3)-RIPL competent cells (Agilent Technologies, #230280). A single colony was picked and inoculated in 1 mL of terrific broth (TB ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression plasmids were transformed into Escherichia Coli strain BL21-Codonplus(DE3)-RIL (Stratagene). The expression and purification of each protein was performed as described previously.77 Briefly ...
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bioRxiv - Biochemistry 2024Quote: The codon-optimized plasmid for hPLIN1 was expressed in Escherichia coli ArcticExpress (DE3) (Agilent) while codon optimized plasmids hPLIN2 and hPLIN3 were expressed in E ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries for whole-exome sequencing were prepared using the SureSelect Human All Exon V7 kit (Agilent) and the Illumina TruSeq Exome kit ...
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bioRxiv - Cell Biology 2020Quote: ... The samples are hybridized on the microarray slide (4×44K Human Genome CGH Microarray, Agilent Technologies). The slides were scanned using an Agilent dual laser DNA microarray scanner G2566AA ...
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bioRxiv - Cell Biology 2020Quote: ... and prediluted polyclonal Guinea Pig anti-human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) as second and third primary antibodies for 1h at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... These data were obtained with Agilent SurePrint G3 Human GE v2 8×60K microarray (Agilent Technologies) from peripheral blood samples (all samples had RNA integrity number (RIN ...
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bioRxiv - Cancer Biology 2021Quote: The sequencing libraries were prepared and captured using SureSelect Human All Exon V4 kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... whole-exome DNA was capture using the SureSelect Human All Exon Kit V5 or V6 (Agilent) and high-throughput sequencing was conducted using the Illumina X10 with a coverage more than 100 X ...
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bioRxiv - Genomics 2020Quote: ... LECs were washed twice in DPBS and stained with mouse anti-human Ki-67 antibody (Dako) diluted 1:800 in FACS buffer (DPBS with 1mM EDTA and 2% FBS ...
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bioRxiv - Systems Biology 2019Quote: ... plasma samples were assayed using quantitative western blotting using a polyclonal antibody to human RBP4 (Dako) and human TTR (Dako ...