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Citations for Agilent :
501 - 550 of 954 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and 90 of human ATG12 were mutagenized into arginine using site-directed mutagenesis following the manufacturer’s instructions (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... target enrichment was performed using the Agilent SureSelect Human All Exon V6 design (Agilent, Santa Clara, CA, USA).
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bioRxiv - Genetics 2019Quote: ... Total human brain RNA was derived from post mortem cortex and provided by Agilent (Agilent Technologies, cat: 540143). For the preparation of mouse brain RNA ...
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bioRxiv - Developmental Biology 2019Quote: ... All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako, M0823, 1:100) and Alexa488-conjugated goat anti-mouse secondary antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: Two-channel microarray experiment was performed with two replicates using the Agilent Whole Human Genome Oligo Microarray (Agilent, catalog #G4851C Whole Human Genome Microarray 8×60K) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA of tumor and matched germline samples were captured using the SureSelect Human All Exon v7 (Agilent) or xGen Exome Research Panel v2 (IDT) ...
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bioRxiv - Biochemistry 2022Quote: Aliquots of 20 µL of human plasma were depleted on a 1260 Infinity Bio-inert LC system (Agilent) coupled to a depletion column (human 14 multiple affinity removal column ...
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bioRxiv - Genomics 2021Quote: ... Exome capture was performed using a SureSelect Human All Exon V6 capture kit (Agilent Technologies, Santa Clara, CA). Samples were sequenced on a HiSeq 4000 next-generation sequencing system (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide microarrays for human whole genome (G4858A design 072363, 8×60k chips SurePrint G3 unrestricted GE, Agilent Technologies) were used for global gene expression analysis ...
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bioRxiv - Biochemistry 2020Quote: ... 2009). MEKK1 542–888 construct (H. sapiens MEKK1 TOG) was amplified from the MegaMan human transcriptome library (Agilent). The TOG construct was cloned into pETNKI-his-3C-LIC-kan vector ...
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bioRxiv - Molecular Biology 2019Quote: ... The R1285Q mutant of human FANCI (FANCImut) was generated by using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent), and was cloned with a N-terminal cleavable Flag tag into the pCDNA 3.1 vector ...
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bioRxiv - Genetics 2019Quote: ... and exome DNA was enriched with an Agilent SureS elect Human All Exon V5 kit (Agilent Technologies, USA). Paired-end sequencing was performed on Illumina HiSeq 4000 (Supplementary Table 1 and 2).
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bioRxiv - Physiology 2021Quote: Mutations were introduced into human Cx50 cDNA in a SP64T vector with QuikChange Site-Directed Mutagenesis Kit (Stratagene) by using PCR primers that amplified the whole plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
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bioRxiv - Molecular Biology 2021Quote: ... for this we used 1 mL column of NHS-activated Sepharose coupled to rabbit anti-human TTR (Dako). The column was washed with PBS and then incubated with 500 μL of human plasma for 2 hours at RT ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and incubated with horse radish peroxidase (HRP) conjugated goat-anti-human IgG antibody (Dako, P0447) (diluted 1:1,600 in PBS/0/1% BSA ...
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bioRxiv - Genetics 2020Quote: ... The whole exome was captured by an Agilent SureSelect All Human exon v5.0 kit (Agilent, Santa Clara, US.), and sequenced on an Illumina Hiseq X-10 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Paired immunofluorescence staining was performed by applying a polyclonal rabbit anti-human CD3 antibody (1/50, A0452, Agilent) and rat IgG2a anti-human Foxp3 (clone PCH 101 ...
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bioRxiv - Neuroscience 2022Quote: ... CD20 (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:200 Clone KP1, Dako Omnis, Code Number: M0814) and CD68 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies for CD3 (Polyclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:200, Dako Omnis, Code Number: GA503), CD20 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Cancer Biology 2023Quote: ... which recognises residues 1978-2016 of human NaV1.5) and stained with EnVision+ Dual Link System/HRP (DAB+; Dako), following the manufacturer’s instructions and counterstained with Mayer’s haematoxylin ...
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bioRxiv - Cancer Biology 2023Quote: ... Quick Amp Labeling Kit and SurePrint G3 Human Gene Expression 8×60Kv3 Microarray (Cat. No. G4851C, Agilent Technologies) was used corresponding to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Positive control PhIP-seq designed to capture mouse and human GFAP began by incubating 0.1µg/mL polyclonal anti-GFAP antibody (Dako) with the murine PhIP-seq library.
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The sequence library was prepared using a Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced using a 2500 Illumina with 125-bp paired-end reads (Illumina ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the homology arms containing plasmid was mutated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biophysics 2020Quote: ... a point mutation was introduced into the WT plasmid using the QuikChange site-directed mutagenesis method (Agilent). HEK-293 cells were transiently transfected with NaChBac WT or T220A cDNA using Lipofectamine™ 2000 transfection reagent (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The required vector was transformed into Escherichia coli BL21 (DE3) cells containing the RIL Camr plasmid (Stratagene) for expression analysis.
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bioRxiv - Microbiology 2022Quote: ... and were used to PCR amplify around the pMMB67EH plasmid using PfuUltra II high-fidelity polymerase (Agilent). PCR products were purified (QIAGEN ...
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bioRxiv - Biophysics 2019Quote: ... Histone expression plasmids were from Narlikar lab and BL21(DE3)pLysS competent E.coli cells were from Agilent Technology (USA) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... point mutations were introduced in the pIRES2-mCherry-YFPCFTR plasmid using site-directed mutagenesis (Quikchange protocol, Stratagene).
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bioRxiv - Cell Biology 2019Quote: Generation of pGDB-SDEL plasmid was done using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) on the pGDB plasmid with primers P1 and P2 per the manufacturer’s protocol36.
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bioRxiv - Microbiology 2021Quote: ... berghei gDNA using primers 9483/9484 and 9485/9486 respectively and introduced sequentially into pBSKS+ plasmid (Stratagene) at XhoI/BamHI and EcoRI/SacII restriction sites respectively ...
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bioRxiv - Biophysics 2022Quote: ... Site-directed mutagenesis were introduced into plasmids using QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) and confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: Plasmids were isolated from 250 mL cultures of Escherichia coli (XL10-Gold Ultracompetent Cells, Agilent Cat. 200314) and 60 μg of each plasmid was used to transfect ring stage parasites ...
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bioRxiv - Neuroscience 2022Quote: ... The pT7-7 plasmid was also modified using site directed mutagenesis (#200523, QuikChange II, Agilent, Waldbronn, Germany) to incorporate a cysteine residue at position 141 to permit dye-labelling of the aSyn protein ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Plasmid and cDNA libraries were pooled and quality was evaluated using capillary gel electrophoresis (Agilent Bioanalyzer 2100). Sequencing was performed on an Illumina HiSeq 1500 instrument using a single-index ...
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bioRxiv - Developmental Biology 2023Quote: ... and the fragments were incorporated into a pBluescript II SK (−) vector plasmid (Agilent Technologies Japan, Hachioji, Japan). Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... The mutation A243V was introduced into the GluN2A plasmid using the QuickChange II XL kit from Agilent. All portions of the resulting construct that were subject to PCR were confirmed by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... I5S mutant (mt) RIIα-mTb-V5 plasmid was generated by site directed mutagenesis (QuikChange II XL, Agilent) based on the wild type (WT ...
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bioRxiv - Microbiology 2023Quote: Plasmids were isolated from 250 mL cultures of Escherichia coli (XL10-Gold Ultracompetent Cells, Agilent Cat. 200314) and 60 µg of pDC2-MORC-HA or linearized pKD-MORC-HA-TetR-DOZI ...
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bioRxiv - Cell Biology 2024Quote: The Nir1-LNS2 plasmid was transformed into competent BL21 (DE3) RIPL cells (Agilent Technologies, Cat. No. 230280) for protein overexpression ...
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bioRxiv - Microbiology 2023Quote: ... Site-directed mutagenesis on the pmPol I-LASV Sag plasmid was performed using QuikChange II kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 39494) with the following dual-color design ...
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bioRxiv - Genomics 2019Quote: ... 37,608 individual biotinylated RNAs targeting the ends of 22,076 promoter-containing human HindIII restriction fragments) were prepared according to the manufacturer’s instructions (SureSelect Target Enrichment, Agilent Technologies). The Hi-C library DNA was denatured for 5 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...