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Citations for Agilent :
51 - 100 of 1893 citations for Chromosome 11 Open Reading Frame 53 C11ORF53 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... An indirect biotin avidin diaminobenzidine (DAB) system (Dako, Glostrup, Denmark) was used for detection ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with HRP-conjugated streptavidin–biotin complex (DAKO). Substrate was developed with DAB (DAKO)and pictures were produced using a Nikon Eclipse E800microscope with a Nikon DXM1200 digital camera (Nikon ...
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bioRxiv - Immunology 2020Quote: ... were introduced into the BG505 SOSIP.v5.2 pPPI4 vector 53 using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). The R500A and Q658K mutations were added to the BG505 SOSIP.v5.2.N241.N289 vector ...
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bioRxiv - Microbiology 2022Quote: ... 25 μl of ds-cDNA was brought to a final volume of 53 μL in elution buffer (Agilent Technologies) and sheared on a Covaris LE220 (Covaris ...
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Immunology 2020Quote: ... Hierarchical tree analysis was performed using Genespring GX 11 (Agilent Technologies, Waldbronn, Germany).
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bioRxiv - Microbiology 2020Quote: ... the sections were incubated with a streptavidin-biotin-HRP complex (DakoCytomation) and the brown color was developed with diaminobenzidine tetrahydrochloride (DAB ...
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bioRxiv - Molecular Biology 2019Quote: ... The C-terminal hexahistidine tag was brought in frame by QuikChange II XL site-directed mutagenesis (Agilent #200521) using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... A 140 bp region of alpha satellite sequence from chromosome 4 and a 349 bp region of HSATII from chromosome 7 were independently cloned into pTargeT™ DNA backbone via StrataClone TA cloning (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Genomics 2020Quote: ... integrity and purity of total RNA were validated by both Nanodrop readings (NanoDrop Technologies Inc.) and BioAnalyzer 2100 (Agilent Technologies). DNA contaminants were removed using a DNAse RNase-free kit (Ambion) ...
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bioRxiv - Bioengineering 2020Quote: ... inserts were transferred to a new clear bottom plate where fluorescence was immediately measured using a bottom reading fluorescence plate reader (BioTek, Agilent) at 485/535 nm (Ex/Em) ...
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bioRxiv - Microbiology 2023Quote: ... The optical density was measured at 540 nm and subtracted from readings at 450 nm using the BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Concentrations were calculated from standard curves run in parallel.
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bioRxiv - Biochemistry 2024Quote: ... The signals were obtained with 8 cm-1 spectral resolution (unless specified otherwise) by performing 35 consecutive readings per measurement in 4,000-650 cm-1 range using MicroLab PC software (Agilent Technologies) and further processed with Spectragryph (F ...
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bioRxiv - Cell Biology 2019Quote: SUMO E1 enzymes Uba2 and Aos1 (from vectors pET11a-UBA2 and pET28a-AOS1, [53]) were co-expressed in BL21 CodonPlus (DE3) RIL (Agilent Technologies) in the presence of 0.05 mM IPTG at 18 °C for 20 h ...
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bioRxiv - Cancer Biology 2020Quote: ... endogenous biotins and avidins were blocked with corresponding blocking solutions from Dako® ...
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bioRxiv - Neuroscience 2020Quote: ... and immunoreactivities were visualized by the biotin–streptavidin technique (ABC kit; Dako) with 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin-containing handles were made using a PCR on pBluescript SK+ (Stratagene) with GoTaq G2 DNA polymerase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... MIC values were determined at 18 hours post-inoculation by reading optical density at 600 nm (OD600) on a Synergy Neo2 Multi-Mode Plate Reader (Agilent BioTek), and inhibition of growth defined as OD600<0.5 above background ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were amplified with 11 PCR cycles and the library size was analyzed by Agilent Tape Station (G2938-90014 ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes with cells were then gently cut out of the Transwell frame with a surgical scalpel blade and mounted with Dako Mounting Medium (Agilent). Images were obtained at 20x and 63x magnification using a Zeiss LSM-780 confocal microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... then spectrometry readings were collected every 5 min for 1 h using a BioTek Epoch microplate spectrophotometer (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cancer Biology 2019Quote: ... the slides were incubated with biotin coupled goat anti-rabbit (1:500, Dako). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Biotin-labeled FcRs were then incubated with streptavidin-PE (Prozyme, Hayward, CA, USA) for 10 min and excess streptavidin was quenched with 20 μM biotin (Avidity ...
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bioRxiv - Plant Biology 2023Quote: ... The slides were then incubated with biotin-conjugated anti-rabbit Ig (Dako E0432) for 30 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... on the DS-11 FX instrument (Denovix) and evaluation of DNA integrity on FEMTO Pulse (Agilent), 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode ...
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bioRxiv - Neuroscience 2022Quote: ... and the plate was incubated at 37°C for 30 minutes before reading at 562 nm on a Synergy plate reader (Agilent, Santa Clara, CA). Using the resulting protein concentration ...
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bioRxiv - Biophysics 2023Quote: ... we constructed the 5’-biotin handles by a PCR from a pBluescript SK+ (Stratagene) using 5’-biotin primers JT337 and JT338 (Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-biotin handles were constructed by a PCR reaction from a pBluescript SK+ (StrataGene) using 5’-biotin primers JT337 (Bio-AGAATAGACCGAGATAGGGTTGAGTG ...
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bioRxiv - Immunology 2024Quote: ... C4b deposition was measured using a biotin-tagged anti-C4c polyclonal Ab (Agilent, Q0369) followed by HRP-streptavidin and TMB One ...
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bioRxiv - Microbiology 2023Quote: ... of which 11-15 samples were also run on the Bioanalyzer using the HS DNA chip (Agilent).
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bioRxiv - Molecular Biology 2020Quote: ... Amplified libraries were quantified using fluorometry with DS-11 (DeNovix, United States) and Bioanalyzer 2100 (Agilent, United States). Diluted libraries were clustered on a pair-read flowcell and sequenced using a NovaSeq 6000 system (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplified libraries were quantified using fluorometry with DS-11 (DeNovix, United States) and Bioanalyzer 2100 (Agilent, United States). Diluted libraries were clustered on a pair-read flowcell and sequenced using a NovaSeq 6000 system (Illumina ...
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bioRxiv - Genomics 2019Quote: ... 11 a 2100 Bioanalyzer instrument was used with an RNA 6000 Pico Assay (Agilent, Santa Clara, CA, USA). We fluorometrically determined the concentration of total RNA with a Quant-iT RiboGreen RNA Assay Kit (Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... Total RNA was quantified by nanodrop (DeNovix DS-11) and TapeStation 4200 (RNA Screen Tape; Agilent Technologies, Inc) or 2100 Bioanalyzer (Total RNA Nano ...
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bioRxiv - Genomics 2024Quote: ... measured on DS-11 FX (Denovix) and size distribution checked using FEMTO Pulse (Agilent, M5330AA & FP-1002-0275.) HMW DNA was sheared with Megaruptor 3 (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... and a commercially available tyramide-based avidin-biotin amplification system (Catalyzed Signal Amplification System, DakoCytomation). IRDye 680RD streptavidin (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by labeling with streptavidin biotin/horseradish peroxidase (LSAB2 System HRP, Dako, Santa Clara, USA) and Diamino-benzidine (DAB ...
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bioRxiv - Genomics 2022Quote: ... RNA was quantified using DeNovix DS-11 Spectrophotometer while the RNA integrity was checked on a Bioanalyzer 2100 (Agilent). Only samples with RIN value above 8.0 were used for transcriptome analysis ...
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bioRxiv - Immunology 2020Quote: ... as described previously (11) or by using Protein G cartridges on the AssayMAP Bravo (Agilent Technologies, Santa Clara, USA) Briefly ...
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bioRxiv - Genomics 2021Quote: ... RNA was further quantified using DeNovix DS-11 Spectrophotometer while the RNA integrity was checked on Bioanalyzer 2100 (Agilent). Only samples with RIN value above 8.0 were used for transcriptome analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was further quantified using DeNovix DS-11 Spectrophotometer while the RNA integrity was checked on Bioanalyzer 2100 (Agilent). Only samples with RIN value above 8.0 were used for transcriptome analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... libraries were amplified for 13 cycles using 2 µl each primer per 50 µl reaction before two rounds of AMPure bead purification at 0.9x and elution in 11 µl 0.1x TE prior to quality control using a Bioanalyzer HS DNA ChIP (Agilent) and quantification using a KAPA Library Quantification Kit (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... libraries were amplified for 9 cycles using 2 µl each primer per 50 µl reaction before two rounds of AMPure bead purification at 0.9x and elution in 11 µl 0.1x TE prior to quality control using a Bioanalyzer HS DNA ChIP (Agilent) and quantification using a KAPA Library Quantification Kit (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... libraries were amplified for 13 cycles using 2 µl each primer per 50 µl reaction before two rounds of AMPure bead purification at 0.9x and elution in 11 µl 0.1x TE prior to quality control using a Bioanalyzer HS DNA ChIP (Agilent) and quantification using a KAPA Library Quantification Kit (Roche).
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... or manually using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, DakoCytomation). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Immunology 2023Quote: ... Tetramers were assembled by conjugating the biotin-labeled monomers with PE-labeled streptavidin (Agilent, #PJRS27-1) according to NIH Tetramer Facility protocols ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then diluted 1:11 in ultrapure water before analysis with ICP-OES (Vista-PRO CCD Simultaneous ICP-OES; Agilent). Calibration was carried out using standards of Zn ...