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Citations for Agilent :
1 - 50 of 1893 citations for Chromosome 11 Open Reading Frame 53 C11ORF53 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Pathology 2020Quote: ... Mutations required for domain excision and/or punctual changes within P1 nucleotidic sequence were introduced in the P1 open reading frame using the QuikChange® II XL Site-Directed mutagenesis kit (Agilent-Stratagene) and related primers (Appendix Table 1) ...
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bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
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bioRxiv - Plant Biology 2019Quote: The open reading frame of cDNA encoding full-length HaHB4 cloned in the BamHI/SacI sites of pBluescript SK- (Stratagene, Upsala, Sweden) was used as template in a PCR reaction with oligonucleotides H4-F (5’ ATGTCTCTTCAACAAGTAACAACCACCAGG-3’ ...
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bioRxiv - Pathology 2020Quote: ... Mutations required for domain excision and/or punctual changes within P1 nucleotidic sequence were introduced in the P1 open reading frame using the QuikChange® II XL Site-Directed mutagenesis kit (Agilent-Stratagene) and related primers (Appendix Table 1) ...
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bioRxiv - Biophysics 2021Quote: ... with second antibody Rb-aMs/biotin (DAKO) for 1h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Gap43 antibodies were visualized with biotin-conjugated rabbit anti-mouse antibodies (Dako) followed by streptavidin-conjugated Cy3 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... 53 using the AdEasy Adenoviral Vector System (Agilent). Vector purification was performed via ultracentrifugation using two sequential cesium chloride gradients ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a mouse secondary antibody (rabbit polyclonal anti-mouse biotin antibody, E0413, Dako, 1:200). Histopathological studies of GBM xenograft mice were conducted by Division of Neuropathology ...
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bioRxiv - Cancer Biology 2021Quote: ... biotin (DakoCytomation), and an additional serum-free protein block (DakoCytomation ...
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bioRxiv - Immunology 2024Quote: ... HRP-conjugated or biotin-conjugated secondary antibodies and ABComplex alkaline phosphatase (Dako) were used as secondaries ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr4 CEP 613kb green for the centromere of chromosome 4 (#G101066G, Dako), FISH RAMP1 red for the RAMP1 gene (#G110996X-8 ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr2 CEP 448kb green for the centromere of chromosome 2 (#G101089, Dako), SureFISH PADI2 red for the PADI2 gene (#G110996X-8 ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr1 CEP 541kb green for the centromere of chromosome 1 (#G101064G, Dako). Prior to use ...
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bioRxiv - Plant Biology 2022Quote: ... The open petri dish was placed inside a UV Stratalinker 2400 (Stratagene) and exposed to set UV light ...
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bioRxiv - Molecular Biology 2024Quote: The antigen-antibody complex was detected by the Labeled Streptavidin–Biotin (LSAB) staining method using a biotinylated goat anti-rabbit antibody (DakoCytomation), subsequently conjugated with streptavidin-horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2021Quote: ... avidin-biotin blockade was performed using the avidin-biotin blocking kit (DAKO, Glostrup, Denmark). To eliminate nonspecific protein interactions with the primary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Analysis and peak integration were performed using Open LAB CDS Chem station software (Agilent). Lysate of cord blood RBCs and adult peripheral blood RBCs from healthy donors were used as controls.
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bioRxiv - Neuroscience 2020Quote: ... we used the endogenous biotin blocking procedure (Avidin/Biotin Blocking Kit, Dako, code No. X0590) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and raw frame data were integrated using CrysAlisPro (Agilent Technologies, 2010). The structures were solved by direct methods with SHELXT 55 and refined by full-matrix least-squares techniques against F2 (SHELXL-2018/3 ...
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bioRxiv - Biochemistry 2021Quote: ... Readings were taken every hour using a UV/vis spectrophotometer (Cary 60, Agilent).
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bioRxiv - Microbiology 2023Quote: ... and OD600 readings were taken using a Synergy LX plate reader (Agilent BioTek).
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bioRxiv - Microbiology 2023Quote: ... and OD600 readings were taken using a Synergy LX plate reader (Agilent BioTek).
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bioRxiv - Bioengineering 2023Quote: ... and luminescence intensity reading was obtained using Synergy H1 microplate reader (Agilent Technologies). Similarly ...
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bioRxiv - Cancer Biology 2019Quote: ... biotin and peroxidase blocking reagents (Dako) prior to primary antibody incubation and then with Bio-Rad Amplification Reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... biotin and peroxydase blocking reagents (Dako) before saturation with TBS containing 0.1% Tween-20 and 5% BSA (TBST-BSA) ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation lasted 2h and was followed by avidin-biotin complexes for signal amplification (ABC kit, Dako). Immunoreactivities were visualized using 3,3’-diaminobenzidine as a chromogen ...
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bioRxiv - Cancer Biology 2020Quote: ... 11 μl of 10X GE Blocking agent (Agilent, Germany), 4 μl of 25% (v/v ...
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bioRxiv - Pathology 2024Quote: ... Labelings were visualized using secondary antibody coupled to streptavidin-biotin peroxidase complex and 3,3’-diamino-benzidine chromophore using the EnVision Flex system (#K8023, DAKO) or the VECTASTAIN Kit (#PK-7800 ...
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bioRxiv - Immunology 2021Quote: ... followed by avidin and biotin blocking (Dako) before staining with the second tetramer ...
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bioRxiv - Cell Biology 2023Quote: Three gRNA libraries with different frames were synthesized as oligo pools from Agilent. Each gRNA library was then PCR amplified from the initial oligo pool and cloned into the modified pSB700 vector using Golden Gate assembly as previously described31.
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bioRxiv - Genomics 2020Quote: ... DNA was eluted into 53 µl with 1 µl used for D1000 TapeStation analysis (Agilent) and 2 µl used for Qubit quantification (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Open dishes without a lid were placed on an ice-cold aluminium plate in Stratalinker 2400 (Stratagene) and 254 nm UV light was applied for 30 seconds to cross-link the protein-RNA interactions ...
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bioRxiv - Plant Biology 2021Quote: ... After reading absorbance at 535 nm on a PowerWave XS2 plate reader (Agilent, former BioTek), FeIIFerroZine concentrations were calculated using an extinction coefficient of 28.6 mM−1 cm−1 (Gibbs ...
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bioRxiv - Immunology 2023Quote: ... and concentration and purity of constructs were validated using absorbance readings at 280nm (Agilent Technologies), Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... OD600 readings were taken every 15 minutes in a Biotek Synergy H1 microplate reader (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... using a Bravo automated pipette liquid handler (Velocity 11/Agilent). Media from cell plates were aspirated using an ELX plate washer ...
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bioRxiv - Evolutionary Biology 2019Quote: FISH with a commercial PNA probe for TTAGGG sequence was performed on metaphase slides and slides with male pachytene chromosomes according to the protocol provided by manufacturer (DAKO). Comparative genome hybridization (CGH ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti- mouse biotin (Dako Denmark, Glostrup, Denmark) was used as a secondary antibody and samples were incubated for 20 min ...
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bioRxiv - Physiology 2023Quote: ... UV readings were performed by using a BioTek Epoch microplate spectrophotometer (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Cancer Biology 2020Quote: ... an avidin/biotin blocking system (Dako Cytomation, Carpinteria, CA), and an additional serum free protein block (Dako Cytomation ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with HRP-conjugated streptavidin-biotin (DAKO). The substrate was developed using diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in avidin-biotin complex (ABC, Dako) for 30 minutes and the antigen-antibody reaction was visualized using di-aminobenzidine (DAB ...
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bioRxiv - Immunology 2024Quote: ... a biotin-tagged polyclonal anti-C1q Ab (Agilent, A0136) was incubated for 1 hr at RT followed by HRP-coupled streptavidin 1:10000 and TMB One.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 90 minutes in a biotin conjugated secondary antibody (a goat anti-rabbit, a goat anti-mouse or a swine anti-rabbit as appropriate; Dako; diluted 1:500) and for 1h in a peroxidase conjugated extravidin (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... To obtain the inward open state the mutations E162Q and D344N were introduced using the quickchange site-directed mutagenesis kit (Agilent). Transformed Saccharomyces cerevisiae (strain DSY-5 ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT N-terminal point mutants were generated from pCR4 TOPO NOCT (Open Biosystems) and Quikchange site directed mutagenesis PCR (Agilent) to generate NOCT(1-431 ...
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bioRxiv - Neuroscience 2019Quote: ... the avidin-biotin-peroxidase complex (1:300; Dako, Glostrup, Denmark) was applied for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... biotin-conjugated donkey anti-rabbit IgG (E0432, Dako, 1:200), and Streptavidin-HRP (P0397 ...