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Citations for Agilent :
601 - 650 of 1271 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 4% PFA and sequentially stained with mAbs to NPM (anti-human/mouse nucleophosmin (NPM) (clone 376, dilution 1:50, Dako) and anti-mouse MPO (Millipore) ...
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bioRxiv - Genomics 2021Quote: ... the initial concentrations of purified SARS-CoV-2 and the universal human reference RNA (UHRR, Agilent Technologies, product number 740000) were determined by ddPCR as described above ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... exome enrichment was performed using the SureSelect Human All Exon 50 Mb Kit V5 (Agilent Technologies, Santa Clara, CA, USA). Sequencing was done on a HiSeq4000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-GFAP immunohistochemistry (Polyclonal Rabbit Anti-Human, Proteinase K enzymatic antigen retrieval, dilution 1:1000, DAKO Omnis, Code Number: GA524) was employed to assess reactive astrogliosis ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 minutes with the rabbit anti-human HES1 antibody diluted 1:100 in a low background diluent (Dako). A linker rabbit was used to amplify the signal ...
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bioRxiv - Genomics 2023Quote: ... Hybrid capture of exonic regions was performed using Agilent SureSelect in-solution capture reagents and SureSelect XT Human All Exon V4 probes (Agilent). Captured libraries were sequenced using 100 bp paired-end runs on Illumina HiSeq 2500 ...
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bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... exome capture was performed on 200 ng DNA using a customised version of the Agilent Human All Exome V5 Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cytokeratin 5/6 (mouse monoclonal) at 1:100 to 1:200 dilution for zebrafish tissue and 1:100 dilution for human tissue (Dako), GATA 3 (SAB2100898 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed using the Exome Cancer Test v2.0 (EXaCT-2) assay that was developed with Agilent based on SureSelect Human All Exon V6 (Agilent Technologies) (manuscript in preparation) ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Immunology 2024Quote: To accurately investigate the bioenergetic function of bovine and human MØ with an extracellular flux analyser (Seahorse Bioscience, Inc, USA), both cell types were characterized according to the manufacturer’s recommended basal and test conditions (23).
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Bound ACPA IgG was detected by incubation with horseradish peroxidase-labelled rabbit anti-human IgG (P0214; Agilent Dako, CA, USA) diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Patient M03’s DNA was captured with the SureSelect Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced on a NextSeq500 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA genomic libraries were prepared from fresh tissue (tumor and nontumor) and FFPE tumor tissue DNA prior to exome capture (SureSelect XT Human All Exon v5, Agilent). Libraries were sequenced on a HiSeq2000 instrument (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Whole exome sequencing on genomic DNA of the patient was performed using the SureSelect Human All Exon V6 kit (Agilent) on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5% (v/v, no unit definition available) AffinityScript Multiple Temperature Reverse Transcriptase (Agilent). RNA was incubated at 65°C for 5 min and was then left at RT for 10 min for primer annealing ...
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bioRxiv - Cell Biology 2019Quote: Cleaved Caspase-3 staining required 5 minute treatment with Target Retrieval Solution (DAKO S1700), 1 hour room temperature incubation with Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 µm-thick paraffin sections were deparaffinized and autoclaved in target retrieval solution (DAKO) for 10 min at 121°C ...
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bioRxiv - Biochemistry 2020Quote: ... The non-specific binding was blocked for 30 min with 5% rabbit serum (DakoCytomation) for the primary mouse serum(1:600) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were blocked and incubated first with anti CD3 antibody (Dako, Supplementary Table 5) and subsequently with horseradish peroxidase conjugated anti rabbit antibody (Dako) ...
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bioRxiv - Cancer Biology 2022Quote: 5 × 104 cells were plated on XF24 cell culture plate (Agilent, no. 100777-004) with DMEM supplemented with 1 % FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Luminescence was determined using the BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent). Cell viability analysis was performed on GraphPad Prism 9 and IC50 values were determined using a nonlinear regression curve fit ...
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bioRxiv - Synthetic Biology 2022Quote: ... Both oligo libraries (Round 5, and Round 6 + mutational scanning) were purchased from Agilent.
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bioRxiv - Plant Biology 2019Quote: ... connected to a Zorbax SB-C18 analytical guard column (5-µm, 4.6 × 12.5mm; Agilent), 0.1% formic acid (v/v ...
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bioRxiv - Microbiology 2019Quote: ... equipped with a C18 reversed-phase column (4.6 × 250 mm, 5 μm; Agilent Technologies) was used for HPLC analysis ...
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bioRxiv - Cell Biology 2019Quote: ... The sections were stained as follows: 1) blocking endogenous peroxidases for 5 min (DAKO Real Peroxidase Blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent, 200474-21) and anti-Rat (Licor ...
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bioRxiv - Microbiology 2021Quote: ... Agent SB-Aq reverse-phase column (250 mm, 4.6 mm i.d., 5 mm; Agilent) was used to perform HPLC at 30°C ...
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bioRxiv - Cancer Biology 2022Quote: ... using the following protocol: Endogenous Enzyme Block for 5 min (FLEX Peroxidase Block, Dako), primary antibody (HER2 A0485 and Melan-A IR633 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with a HP-5 Ultra Inert column (Agilent, 30 m × 0.25 mm, 0.25 µm). 1μl of each sample was injected using Agilent ALS 7694 autosampler in split mode with a 5:1 ratio with helium as the carrier gas (250°C injector temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent images of severed axons were acquired with the Cytation 5 automated microscope (Agilent). Within one experimental replicate ...
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bioRxiv - Microbiology 2022Quote: ... and 20% glycerol at 30°C in a BioTek Cytation 5 imaging reader (Agilent) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were desalted using solid phase extraction C18 cartridges (Agilent, 5 μL bed volume). Then ...
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bioRxiv - Biophysics 2023Quote: ... we constructed the 5’-biotin handles by a PCR from a pBluescript SK+ (Stratagene) using 5’-biotin primers JT337 and JT338 (Table S1) ...
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bioRxiv - Cell Biology 2023Quote: ... both packed with a Zorbax 300SB 5 μm C18 phase (Agilent Technologies, Waldbronn, Germany). The mobile phase was composed of H2O/FA (100:0.1 ...
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bioRxiv - Genomics 2023Quote: ... cell counts were measured using a Cytation 5 Cell Imaging Multimode Reader (Agilent BioTek).
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bioRxiv - Genomics 2023Quote: ... 5 ng of each RNA sample was analyzed using a 5200 Fragment Analyzer (Agilent) with the High Sensitivity RNA Assay kit (DNF-472) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-biotin handles were constructed by a PCR reaction from a pBluescript SK+ (StrataGene) using 5’-biotin primers JT337 (Bio-AGAATAGACCGAGATAGGGTTGAGTG ...
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bioRxiv - Microbiology 2023Quote: ... The fixed plates were scanned using the high-content imaging system Cytation 5 (Agilent), with either the GFP channel for rVSV-S or the Texas red channel for rSARS-CoV-2 virus ...