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Citations for Agilent :
51 - 100 of 7386 citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... NDE1 and RASAL2 were PCR-cloned into pCMV2B (Flag) and pCMV3B (Myc) constructs (Stratagene). Expression constructs for SCRIBBLE ...
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bioRxiv - Bioengineering 2020Quote: ... anti-SOX17, and anti-SOX9 (MerckMillipore, AB5535, 1:300) antibodies and visualization with EnVision FLEX Mini kit (DAKO).
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with peroxidase-conjugated donkey anti-rabbit and anti-mouse secondary antibodies from a kit (Dako) for 1 hr ...
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bioRxiv - Immunology 2019Quote: ... The sections were incubated in the kit polymer-HRP anti-mouse (Dako En Vision+ System-HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... namely N1 and N2 or the empty pCMV-taq2-FLAG (Stratagene, Santa Clara, CA, USA) vector (VC ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining visualisation was performed by incubating with a cocktail of anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Envision Kit, Agilent) for 60 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
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bioRxiv - Molecular Biology 2021Quote: ... A 2x FLAG tag was introduced upstream of the 2x myc tag by quikchange (Quikchange, Stratagene). Addgene plasmid #25361 was found to have the Arg50His variant not present in consensus Uniprot sequence (Q5S007 ...
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bioRxiv - Cancer Biology 2022Quote: ... For secondary antibody staining, either ImmPRESS kits (anti-goat, MP-7405; anti-rat, MP-7444) or Envision+ Reagents (Dako: rabbit, K4002) were used ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Developmental Biology 2020Quote: ... Sox11-FLAG mutants were created by site-directed mutagenesis via polymerase chain reaction with 2XPfu DNA polymerase (Agilent). Primers can be found in Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... an asparagine at position 1326 of hsNUDT9H-FLAG (DsRed) was replaced with aspartate using QuikChange mutagenesis system (Agilent). Defined oligonucleotides in sense and antisense configuration were obtained from (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with peroxidase-conjugated donkey anti-rabbit secondary antibody from a kit (Dako) for 1 hr ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, Flag-NLS-linker-Cas9 (Baker et al., 2016) was co-electroporated with a pAAV backbone-based plasmid (Stratagene) containing two U6-promotor-driven gRNAs targeting HPRT (GACTGTAAGTGAATTACTT) ...
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bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP ...
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bioRxiv - Biochemistry 2021Quote: Rat Kir6.1 and N-terminal FLAG-tagged (DYKDDDDK) SUR2B were first cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses in HEK293 cells according to manufacturer’s instructions63,64 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting DNA fragments were cloned in frame with the FLAG tag using the oligonucleotides that are indicated in Supplementary file 2 into the pESC-URA vector (Agilent), in which the GAL10 and GAL1 promoters were replaced with the Cup1 promoter ...
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bioRxiv - Cell Biology 2021Quote: ... The jordan and shaker-2 non-synonymous substitutions were separately introduced into pFastbac1 M15-2IQ-EGFP-FLAG by site-directed mutagenesis (QuikChange II, Agilent) and verified by Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-NBR1 was generated by clone the NBR1 sequence from GFP-NBR1 into pCMV-Tag 2C vector (Agilent Technologies, #211172). pGEX6P was purchased from Millipore Sigma ...
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bioRxiv - Biochemistry 2024Quote: Genes encoding rat Kir6.2Q52R and N-terminal FLAG-tagged (DYKDDDDK) hamster SUR1 were cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by an anti-rabbit IgG antibody (MP-7401 ImmPress Reagent Kit) and DAB+ chromogen (Agilent Technologies). Slides were counterstained with hematoxylin Gill’s Formula (H-3401 ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFPC1-FLAG-R258Q hSYNJ1-145 kDa and pEGFPC1-FLAG-R839C hSYNJ1-145 kDa [25] were re-engineered by site directed mutagenesis (Agilent QuikChange 200517) to delete the EGFP using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... coli-optimized synthetic gene encoding the E2 ORF from HPV-16 (GenBank: AAD33255.1) was cloned into pCAL-N-FLAG (Agilent Technologies, CA, USA), which contains a vector-encoded N-terminal calmodulin bind site (CBS) ...
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bioRxiv - Immunology 2019Quote: ... The sections were incubated in the kit polymer-HRP anti-mouse (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark), as secondary antibody ...
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bioRxiv - Pathology 2021Quote: ... Slides were again washed in TBS-T two times followed by incubation with anti-rabbit EnVision detection kit (DAKO) for 1 hour in room temperature ...
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bioRxiv - Pathology 2023Quote: ... then washed in PBS and exposed to the detection kit (EnVision + System-HRP Anti-Rabbit, Dako Denmark A/S) which included 3,3′-diaminobenzidine tetrahydrochloride as chromogen and counterstained with Harris hematoxylin ...
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bioRxiv - Immunology 2023Quote: ... was amplified using primers listed in Table S2 and cloned into SrfI and EcoRI-linearized pCMVtag2B vector (N-terminal FLAG epitope-containing plasmid from Agilent Technologies, Cat# 211172) using Gibson Assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: ... The slides were washed the next day and then incubated with biotinylated anti-rabbit secondary antibody using a DAB kit (Dako) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was carried out with the LSAB2 System-HRP (anti-rabbit/mouse LSAB2 Kit Dako Cat#K0679, dilution 1:100) or the VECTASTAIN Elite ABC HRP Kit (Vector Labs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sections were washed and incubated with a secondary antibody (Polymer-HRP Anti-rabbit and DAB following kit protocol (DC EnVision + System, HRP; DAKO). Slides were counterstained with hematoxylin ...
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bioRxiv - Biochemistry 2022Quote: ... and they were also used as templates for constructing the subsequent METTL11A-FLAG and METTL11B-GFP mutants using the Quikchange site-directed mutagenesis protocol (Agilent Technologies, Santa Clara, CA). The following forward primers and their reverse complements were used ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse and anti-goat antibodies (Dako) were diluted 1:10,000 in 1% BSA/PBST ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-rat and anti-rabbit antibodies (Dako).
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bioRxiv - Microbiology 2021Quote: ... anti-Iba1 (Wako) or anti-GFAP (Dako), polyclonal chicken anti-GFAP (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... anti-mouse and anti-goat antibodies (Dako) were diluted 1:10,000 in 1% BSA/PBST ...
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bioRxiv - Neuroscience 2020Quote: ... the staining was revealed by 30 min incubation in a HRP-anti rabbit polymer system (DAKO REAL Envision™ Kit, #K400311-2, Agilent, France) followed by a DAB revelation of few seconds (DAKO DAB Kit ...
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bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... slices were then incubated with anti-cleaved caspase 3 antibody or anti-Ki67 antibody (Cell Signalling) and further processed with secondary antibody (LSAB2 horseradish peroxidase kit; Dako, Copenhagen, Denmark).
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bioRxiv - Cancer Biology 2023Quote: ... The Telomere PNA Kit/FITC kit from Dako (Agilent) was used to estimate Telomere length signal in samples ...
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bioRxiv - Developmental Biology 2021Quote: ... or anti-mouse (CST) or anti-goat (Dako) for 1 hr RT ...
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bioRxiv - Plant Biology 2021Quote: ... and anti-goat (for anti-CHS) immunoglobulins (Dako) were used as secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD20 and anti-CD19 mAbs (Dako, RTU). The positive controls included lymphoid tissue in the tonsil ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-rabbit and anti-mouse antibody (DAKO-Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit or anti-mouse immunoglobulins (1:3000, Dako), were applied for 1 h at room temperature and detected using SuperSignal chemiluminescent substrates (Pierce).
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN anti-mouse EMD Millipore MAB377, GFAP anti-mouse, PSD-95 anti-rabbit abcam ab18258, S100B anti-rabbit Dako Z0311, SYT-1 anti-goat LSBio LS-B5899), secondary antibodies (488 anti-mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... KIT (Dako/Agilent), p27Kip1(Fisher/BD Biosciences Pharmingen) ...
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bioRxiv - Cancer Biology 2020Quote: ... KIT (Dako/Agilent), p27Kip1(Fisher/BD Biosciences Pharmingen) ...