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Citations for Agilent :
401 - 450 of 6679 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... The IHC staining was performed with a diaminobenzidine (DAB) detection kit (Dako). The multiplexed IF staining was performed following the instruction of Opal 7-color IHC kit (NEL811001KT ...
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bioRxiv - Microbiology 2019Quote: ... and retention time (RT) were imported into a personal compound database and library (PCDL Manager, version B.07.00, Agilent Technologies) used in data processing workflow.
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bioRxiv - Immunology 2019Quote: ... Quantification of the purified RT-PCR products was also performed using an Agilent 2100 Bioanalyzer (Agilent Technologies) according to the Agilent High Sensitivity DNA kit instructions.
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bioRxiv - Biochemistry 2019Quote: ... Plates were sealed using optically clear films and transferred to an Agilent Mx3005p RT-PCR machine (Agilent). The fluorescence intensity was measured during a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR reaction targeting RdRP gene was performed in triplicate in a Mx3005P QPCR System (Agilent) using PrecisionFAST qPCR Master Mix (PFAST-LR-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... For detection the EnVision detection system (K500711-2, Dako) was used.
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bioRxiv - Developmental Biology 2020Quote: ... The signals were visualized using a REAL Streptavidin Alkaline Phosphatase kit (Dako), while Ki-67 staining was visualized with DAB ...
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bioRxiv - Genetics 2022Quote: PCR products were cloned using StrataClone PCR Cloning Kit (Agilent, Waldbronn, Germany). Positive white colonies were randomly picked and verified with colony PCR ...
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bioRxiv - Physiology 2020Quote: ... Real-time qPCR amplification was performed using SYBR GreenER™ qPCR SuperMix Universal (Thermo) or miRNA QPCR Master Mix (Agilent).
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bioRxiv - Neuroscience 2019Quote: ... Real-time verification of the DBS stimulation parameters was monitored using a digital oscilloscope (Agilent 54622D oscilloscope, Agilent Technologies, Amstelveen). Animals requiring stimulation intensities above 100 µA were excluded from the analysis ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed using a Thunderbird SYBR qPCR Mix (Toyobo) and the analysis was carried out with a real-time thermal cycler (Stratagene Mx3000p; Agilent). Primers for quantitative RT-PCR are as follows ...
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bioRxiv - Developmental Biology 2020Quote: ... Gene-specific RT-PCR was performed using the SYBR green master mix (Applied Biosystem) and Agilent MX3005P Multiplex QPCR Real-time Thermal Cycler (Stratagene). The amplification protocol consisted of an initial cycle of 1 min at 95°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time qPCR amplification was performed using SYBR GreenER™ qPCR SuperMix Universal (Thermo) or miRNA QPCR Master Mix (Agilent). The miR-467a primer (GTA AGT GCC TAT GTA TATG ...
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bioRxiv - Immunology 2019Quote: ... Microplates were incubated for 30 min at 37 °C in a non-CO2 incubator and subjected to real-time analysis of OCR using an XF96 Extracellular Flux Analyzer (Agilent). For the mitochondrial stress assay analysis ...
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bioRxiv - Immunology 2021Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using the real-time flux analyzer Seahorse XF24 (Agilent) according to a method modified from Van den Bossche et al ...
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bioRxiv - Neuroscience 2022Quote: Mitochondrial and glycolytic function was analyzed with the Seahorse Cell Mito Stress Test and Seahorse Real-Time ATP Rate Assay on a Seahorse XFe24 Extracellular Flux Analyzer (Agilent). Neurons were treated on day 11 of culture with 500 μL of fresh neuronal media plus 500 μL of astrocytic conditioned media of varying treatment ...
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bioRxiv - Cancer Biology 2022Quote: Mitochondrial oxygen consumption rate (OCR) was measured in real-time using the XFp Extracellular Flux Analyzer (Seahorse Bioscience, MA, USA). Cells were seeded in 6-well plates at density 80 000 cells/well and treated with Tranilast (100 µM ...
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bioRxiv - Cancer Biology 2022Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in real-time using Seahorse (XF24 and XFe96) extracellular flux analyzers (Agilent). Cells were seeded in XF24 (5×104 ...
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bioRxiv - Biochemistry 2023Quote: ... drugs were added at the indicated concentration in triplicate and the cell index was measured over a period of 48-96 h (Real Time Cell Analyzer, Agilent).
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bioRxiv - Pharmacology and Toxicology 2023Quote: Measurements of real-time of oxygen consumption rates (OCR) were determined in whole cells using a Seahorse XF96 extracellular flux analyser (Agilent) as described previously (78) ...
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bioRxiv - Cancer Biology 2023Quote: Oxygen consumption was measured using Oxygen consumption rate (OCR) was measured in real time using the XF96 extracellular flux analyzer (Agilent). AML cells were seeded on Cell-Tak (#10317081 ...
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bioRxiv - Plant Biology 2023Quote: The HRM analysis was carried out using MeltDoctorTM HRM Master Mix on AriaMx G8830A Real-Time qPCR System (Agilent Technologies). Each 20 µl reaction comprised of 10 µl Evagreen HRM master mix ...
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bioRxiv - Neuroscience 2023Quote: ... HLA-DR-and PLP-stained sections were incubated with HRP-labelled anti-mouse antibody (K5007, Dako Real EnVision detection system; Dako, Nowy Sącz, Poland) for 1 hour and immunostaining was visualized with 3’3-diaminobenzidine (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Cell Biology 2019Quote: ... and integrated lentiviral sgRNA sequences were amplified by a two-step PCR reaction (20 cycles and 14 cycles, respectively) as previously described (32, 41) using a Herculase II Fusion DNA Polymerase kit (Agilent biotechnologies). DNA was purified after each of the PCR reactions using a QIAquick PCR purification kit (Qiagen) ...
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bioRxiv - Biophysics 2023Quote: ... PCR site directed mutagenesis kit (Agilent Technologies) was used according to the instruction manual for mutagenesis PCR.
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative RT-PCR and relative quantification analyses were performed with the MX3000P system (Agilent, Santa Clara, CA, USA) using SYBR Green dye chemistry ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNAs were obtained and amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Detection was achieved using the Envision rabbit detection system (Dako) with diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed by using QuantiNova kit on Mx3500P PCR machine (Stratagene). The following oligonucleotide primers were used to detect target gene expression ...
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bioRxiv - Plant Biology 2021Quote: ... Chimeric PCR products were subcloned before sequencing using StrataClone PCR cloning kits (Agilent).
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bioRxiv - Cell Biology 2023Quote: ... at 32°C followed by EnVision Flex+ Mouse LINKER (Dako Omnis) for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was carried out using Thunderbird SYBR qPCR Mix (Toyobo, Japan) with a Stratagene mx 3000p real-time thermal cycler (Agilent, USA). Relative transcript levels were calculated against a standard curve with normalization to the expression of PLANT U-BOX PROTEIN1 (PUB1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Real-Time ATP production rate were measured using the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent Technologies, Santa Clara, CA). To measure glucose-dependent mitochondrial respiration ...
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bioRxiv - Cancer Biology 2021Quote: ... and Real-Time ATP production rate were measured using the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent Technologies, Santa Clara, CA). To measure glucose-dependent mitochondrial respiration ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time oxygen consumption rate (OCR) of these cells was measured using the Seahorse Extracellular Flux (XFe-96) analyzer (Agilent Technologies). Protein concentration of cells in each well was determined ...
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bioRxiv - Bioengineering 2022Quote: ... and lactate-linked proton efflux rate (PER) were obtained in real-time using the Seahorse XFe-96 Extracellular Flux Analyzer (Agilent Technologies)12,13,15 ...
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bioRxiv - Bioengineering 2022Quote: ... and lactate-linked proton efflux rate (PER) were obtained in real-time using the Seahorse XFe-96 Extracellular Flux Analyzer (Agilent Technologies)34–36 ...
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bioRxiv - Systems Biology 2023Quote: The kinetic data in this study are published and were acquired from xCELLigence assays using the xCELLigence Real Time Cell Analysis (RTCA) Single Plate device (Agilent Technologies) (Dankwa et al ...
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bioRxiv - Immunology 2024Quote: ... were sequentially used to treat cells during real-time measurements of extracellular acidification rate (ECAR) using Seahorse XFe24 Analyzer (Seahorse Bioscience). For the mito stress test ...
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bioRxiv - Immunology 2019Quote: ... Calibration beads were stained with F(ab’)2 FITC-Conjugated Goat Anti-Mouse immunoglobulins (Dako, F0479) at 1:50 dilution for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The calibration of TIMS was done with the Agilent ESI LC/MS tuning mix (Agilent Technology). The selected ions for the calibration of positive modes were ...
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bioRxiv - Cancer Biology 2021Quote: ... Na+ content was quantified using certified reference standards (multi-element environmental calibration standard, Agilent, 5183-4688) and a 10 ppb internal standard solution prepared from a certified reference solution (Agilent ...
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bioRxiv - Biophysics 2019Quote: ... Major interfering proteins were removed from the serum by the Multiple Affinity Removal Spin Cartridge HSA/IgG (Agilent Technologies, USA) as described into manual ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit/mouse following manufacturer’s instructions (Detection Kit #K5005, Agilent Technologies, Santa Clara, CA, USA). Immunostained tissue sections were counterstained with hematoxylin solution according to Mayer (T865.1 ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed to amplify the dazl coding region using Easy-A High Fidelity Taq polymerase (600400, Agilent) (primers in Supplemental Table 1) ...
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bioRxiv - Biochemistry 2021Quote: Differential scanning fluorimetry (DSF) analysis was performed in a Stratagene Mx3005P RT-PCR instrument (Agilent, Santa Clara, CA, USA) in thin-walled 96-well PCR plates with transparent Microseal® ‘B’ seals (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... or directly processed by RT-qPCR using Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix (Agilent Technologies), using primers designed with SnapGene® (version 6.1.1 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products of positive clones were cloned with the StrataClone PCR Cloning Kit (Agilent) into the supplied vector system ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were cloned using the StrataClone PCR cloning kit (Cat #240205, Agilent Technologies) and sequenced using T3 and T7 oligonucleotide to confirm absence of wild type allele.