Labshake search
Citations for Agilent :
401 - 450 of 638 citations for 3 Trifluoromethylthio benzeneboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... and branched-chain amino acids (BCAAs) was performed with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described [61] ...
-
bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
-
bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
-
bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
-
bioRxiv - Biochemistry 2022Quote: ... system coupled to an Agilent 6470 series QQQ mass spectrometer (MS/MS) was used to analyze fatty acids in each sample (Agilent Technologies, Santa Clara, CA). The methods are similar to what is described in the paper by Yang ...
-
bioRxiv - Genomics 2024Quote: ... The measurement of the mineral nutrients in the acid digests was conducted by inductively coupled plasma optical emission spectroscopy (ICP-OES; Agilent 5110 Vertical Dual View). The measurements were verified by using certified standard reference material (SRM ...
-
bioRxiv - Immunology 2024Quote: ... The reaction was stopped with trifluoroacetic acid, and peptides were analyzed by RP-HPLC (Shimatzu, Kyoto, Japan) on an AdvanceBio Peptide Plus C18 column (Agilent Technologies, Santa Clara, CA). The samples (100 µL ...
-
bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph, Agilent Technologies, Les Ulis, France) as previously described (25).
-
bioRxiv - Developmental Biology 2021Quote: ... 0.1 x volume of 3M Acetate (pH 5.5) and 3 x volume of 100% ethanol.Library sizes were measured on 2100 Bioanalyzer instrument (Agilent) using the Agilent High Sensitivity DNA kit (Agilent ...
-
bioRxiv - Neuroscience 2021Quote: ... Next day membranes were washed for 3 x 5 min and incubated with HRP-coupled secondary antibodies (DAKO) diluted 1:3000 in washing buffer for 1.5 hour at RT ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells (3 · 104 hPASMC per well) were seeded onto XF96 V3 PS Cell Culture Microplates (101085-004, Agilent) and exposed to air or CSE for 24 hours before being loaded into the analyzer ...
-
bioRxiv - Bioengineering 2021Quote: ... Sections were then incubated for 40 mins in 3% H2O2 TBST solution and blocked with protein block (Dako) for 20 mins ...
-
bioRxiv - Genetics 2020Quote: ... Captured libraries were amplified in 3×100 μl reactions containing 1 unit Herculase II Fusion DNA polymerase (Agilent), 1x Herculase II reaction buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
-
bioRxiv - Molecular Biology 2023Quote: ... The 5’mitoUTR was cloned into the TFAM-mScarlet/pWPXL or the 3’mitoUTR_TFAM-mScarlet_pWPXL plasmid using megaprimers following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol provided by the manufacturer ...
-
bioRxiv - Neuroscience 2022Quote: ... and 3 μM BPTES (Sigma-Aldrich, SML0601) (all XFp Mito Fuel Flex Test Kit, Agilent Technologies 103270-100) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by another containing 3 μM BPTES (all XFp Mito Fuel Flex Test Kit, Agilent Technologies, 103270-100) according to manufacturer’s instructions.
-
bioRxiv - Biochemistry 2024Quote: ... The test compounds were analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column with 0.1% formic acid in water (A ...
-
bioRxiv - Biochemistry 2024Quote: ... and the filtrate was analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column with 0.1% formic acid in water (A ...
-
bioRxiv - Microbiology 2022Quote: ... The optical density (OD) was measured at 405nm using Cytation™ 3 Cell Imaging Multi-Mode Reader (Agilent) and data was analysed using BioTek Gen5 software (Agilent) ...
-
bioRxiv - Plant Biology 2022Quote: ... a Poroshell 120 SB C18 column (3 × 100 mm, 2.7 μm, 4.6 × 100 mm, Agilent, Palo Alto, USA) was utilized during the analysis ...
-
bioRxiv - Genetics 2023Quote: ... slides were carefully rinsed 3 × 5 min with PBS and slides were mounted with Fluorescent Mounting Medium (Dako) and examined under fluorescence using a Zeiss microscope equipped with an AxioCam HRm camera.
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times with PBS and mounted using fluorescent mounting medium for imaging (Dako, #S3023).
-
bioRxiv - Immunology 2024Quote: ... then in 3% hydrogen peroxide for 15min and in serum-free protein block solution (Dako, Cat # X090930-2) for 30min ...
-
bioRxiv - Microbiology 2024Quote: ... Two amino acid substitutions (S299A and T309A) were carried out using specific primers (Table S1) and Pfu Turbo Polymerase (Agilent Technologies, Santa Clara, CA, USA). Then amplicons were further treated with DpnI (New England BioLabs ...
-
bioRxiv - Biophysics 2020Quote: ... Optical absorption spectra of the samples in a 300-900 nm range were recorded within about 10 s at given time intervals (about 0.3-1.0 min) in a 3 mL quartz cuvette (Helma QS1000, 1 cm pathlength) using a Cary 60 spectrometer (Agilent). Alternatively ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: ... analyte separation was achieved using a Synergi Hydro-RP column (2.5 µm 3×100mm, Phenomenax) at ambient temperature on a 1260 Infinity (Agilent)-QTRAP500 (AB Sciex ...
-
bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...
-
bioRxiv - Immunology 2020Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97 °C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2020Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97 °C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2021Quote: HMECs were cultured at a density of 3 x 104 cells/well in 24-well Seahorse XFe24 plates (Agilent) and incubated at 37 °C under a humidified 5% CO2 atmosphere overnight in the presence or absence of DMF ...
-
bioRxiv - Pathology 2021Quote: ... Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Pathology 2021Quote: ... Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... Polyamines were extracted via gravity alone (nominal flow rate of 0.07 mL/min) onto a 1000 mg Bond Elut-C18 SPE column (3 mL, Agilent) pre-conditioned with methanol and bicarbonate buffer at pH 12 ...
-
bioRxiv - Genetics 2020Quote: ... Baseline respiration and maximal respiratory capacity were measured using the Seahorse instrument (XF24 data for Figure 2 and Xe96 data for figure 3) according to manufacturer’s instructions (Agilent). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... The plate was then exposed to a temperature gradient for 3 min in a PCR machine (Agilent SureCycler 8800), followed by 3 min at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... or 100μM W-7 for 30m prior to application of vehicle or 100μM NMDA for 3-5m before crosslinking at 254nm in a Stratalinker 2400 (Stratagene) at 1500mJ/cm2 ...
-
bioRxiv - Cell Biology 2023Quote: ... Myotubes grown on 24 well plates were fasted for 3 h in glucose-free DMEM (103575-100, Agilent, USA). Incubation with 100 nM insulin (Roche Diagnostics ...
-
The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Cyanine 3-labelled cRNA was synthesized from 150 ng RNA using the Low Input Quick Amp Labeling Kit (Agilent) and predicated using RNeasy Mini Kit (QIAGEN ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Size distribution and concentration of 3’ cDNA libraries were verified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). Finally ...
-
bioRxiv - Bioengineering 2023Quote: ... 5 μL of the sample were injected into a C18 reversed phase column (InfinityLab Poroshell 120 EC-C18 2.7 μm 3×100 mm, Agilent) at 40C and detected with an HPLC fluorescence detector (1260 Infinity II Fluorescence Detector ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Chromatographic separation was done on a Shimadzu Nexera X3 equipped with an Agilent 1290 infinity II inline filter (0.3 µm) and a ZORBAX RRHD Eclipse XDB-C18 column (3×50 mm, 1.8 µm; Agilent Technologies). Separation was done in gradient mode with 0.05 % formic acid (Fisher Chemical) ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 µl of the sample was injected onto a BioSEC-3 300 or BioSEC-5 1000 column (Agilent Technologies) pre-equilibrated with PBS or 10 mM histidine ...
-
bioRxiv - Cell Biology 2024Quote: ... Slides were incubated in 2 × SSC containing 0.5 mg/mL Hoechst 33258 for 15 mins in the dark and irradiated for 3 min (5.4 × 105 J/m2, energy 5400) in a UV Stratalinker 2400 (Stratagene). The nicked BrdU/C substituted DNA strands are degraded by Exonuclease III digestion for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: ... 2cm x 100µM) for 5min in solvent A (0.1% formic acid in water) before being separated on an analytical column (Agilent Poroshell, EC-C18, 2.7µM, 50cm x 75µM). Solvent B consisted of 0.1% formic acid in 80% acetonitrile ...
-
bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemical reaction was developed using 3, 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
-
bioRxiv - Cell Biology 2020Quote: ... Frozen samples were spread to a thin layer on petri dishes pre-cooled on dry ice and UV-crosslinked 3 times at 400 mJ/cm2 using a Stratalinker 2400 (Stratagene). All samples were stored at −80°C until tissue lysis.
-
bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...