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Citations for Agilent :
4301 - 4350 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Baseline ECAR and changes in glycolytic rate were assessed using the Seahorse XFp Glycolytic Rate Assay Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA-seq libraries for control bulk RNA were generated using the SureSelect Strand-specific kit (Agilent Technologies, G9691B). Bulk DNA from over 1 × 105 cells was obtained from the cell culture (T0.5 sample ...
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bioRxiv - Plant Biology 2019Quote: ... Site-directed mutagenesis was carried out using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, CA, USA) according to manufacturer's instructions.
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bioRxiv - Molecular Biology 2019Quote: ... The average size and concentration of libraries were determined using the 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent) and qPCR with SensiMix SYBR (Bioline ...
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bioRxiv - Cell Biology 2019Quote: ... the pMTT1-GFP-LF4A plasmid was subjected to site-directed mutagenesis using the QuikChange Lightning kit (Agilent 210518) with the primers 5’-CAGGACGTTTGGCACTAGTGGCTGAATTGATGGATCAGAACC-3’ and 5’-GGTTCTGATCCATCAATTCAGCCACTAGTGCCAAACGTCCTG-3’.
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bioRxiv - Biochemistry 2019Quote: ... coli GyrB overexpression was mutated by site directed mutagenesis using the QuikChange XL Site-Directed Mutagenesis kit (Agilent) in order to generate two plasmids harboring R286K or R286Q mutations ...
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bioRxiv - Molecular Biology 2020Quote: ... Preparations were analyzed using an Agilent 2100 Bioanalyzer and Agilent Small RNA kit (Agilent Technologies, Santa Clara CA).
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bioRxiv - Microbiology 2020Quote: ... pT7/EV-A71BrCr and pCMV/hRV14 by using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). All mutants were verified by sequencing after mutagenesis ...
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bioRxiv - Physiology 2021Quote: ... RNA integrity was verified using an Agilent Technologies 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies). RNA quantity was determined using a Nanodrop ND-1000 spectrophotometer (PEQlab Biotechnologie GmbH) ...
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bioRxiv - Molecular Biology 2020Quote: ... L765A) of this fragment were cloned from this vector using a QuikChange II site-directed mutagenesis kit (Agilent). The DNA construct used for proteins for crystallography was similar except it coded for PARP-1 662-1011 (again with V762A ...
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bioRxiv - Neuroscience 2020Quote: ... PLCγ2-myc-DDK was subjected to site-directed mutagenesis (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent, 210515) to create PLCγ2-P522R-myc-DDK (P522R ...
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bioRxiv - Biophysics 2021Quote: ... and L207W mutations were generated by performing site-directed mutagenesis PCR using Quik Change II Mutagenesis Kit (Agilent) with the following primers:
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bioRxiv - Plant Biology 2019Quote: ... which was Cy3 labelled via the Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA). The labelling efficiency ...
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bioRxiv - Synthetic Biology 2020Quote: ... The assembly products were then re-purified using the Monarch PCR & DNA Cleanup Kit and quantified by Agilent Bioanalyzer (DNA 1000).
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bioRxiv - Molecular Biology 2021Quote: ... The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent) (Figure S5) ...
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bioRxiv - Microbiology 2021Quote: ... and DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp −6000 bp; Agilent, Santa Clara, CA, USA). Average library length was 341 bp ...
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bioRxiv - Microbiology 2020Quote: ... DNA library quality control was performed using a Tapestation 2200 with a High Sensitivity D1000 kit (Agilent Technologies) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Different point mutations of cysteines to serines in GRXS17 were generated using QuikChange II Directed Mutagenesis Kit (Agilent) using primers detailed in Supplemental Table 2 ...
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bioRxiv - Biochemistry 2020Quote: The T2X mutations (T2A, T2R, T2Q and T2S) were generated by site-directed mutagenesis (Agilent Quick Change Kit) using the pMH406 plasmid containing esxB-esxA operon as a template ...
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bioRxiv - Genetics 2020Quote: ... The quality of the purified DNA library was assessed by a Bioanalyzer High-Sensitivity DNA Analysis Kit (Agilent). Illumina HiSeq SBS Kit v4 was used for PE101 sequencing (Illumina).
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bioRxiv - Molecular Biology 2019Quote: ... The obtained libraries were validated and quantified by an Agilent 2100 Bioanalyzer using a DNA7500 LabChip kit (Agilent) and an equimolecular pool of libraries was prepared and titrated by qRT-PCR using the “Kapa-SYBR FAST qPCR kit forLightCycler480” (Kapa BioSystems ...
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Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylatebioRxiv - Biochemistry 2019Quote: ... R206E and R249E were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies). The pEV5HisTEV-can1 wild-type and variant constructs were transformed into C43 (DE3 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA integrity was assessed using Agilent RNA 6000 Nano kit and 2100 Bioanalyzer instrument (both Agilent Technologies, USA). Both fractions were depleted from ribosome RNA using the Ribo-Zero depletion Kit for Gram negative bacteria (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA quality was examined using an Agilent RNA 6000 Nano kit with an Agilent 2100 Bioanalyzer (Agilent Technologies). Two μg of RNA was reverse-transcribed with 0.5 μg oligo(dT)18 primer using an Applied Biosystem™ High-Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... and quantified by qPCR using the KAPA Library Quantification Kit (KapaBiosystems) on a MxPro instrument (Agilent Technologies, USA). The libraries were sequenced using 251 base- length read chemistry in a paired-end flow cell on the Illumina HiSeq2500 sequencer (Illumina ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity was assessed with the RNA Nano 6000 assay kit and the Bio analyzer 2100 system (Agilent).
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bioRxiv - Genetics 2020Quote: ... 1 μL of 4sU-labelled RNA was quality-checked using the Agilent RNA 6000 Pico kit (Agilent technologies) according to the manufacturer’s instructions and run on a 2100 Bioanalyzer Instrument (Agilent).
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bioRxiv - Genetics 2020Quote: ... Mutations were introduced by site-directed mutagenesis of the wild-type gene using the QuikChange mutagenesis kit (Agilent). Sequencing was performed to confirm correct base pair substitutions or deletions were made.
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bioRxiv - Immunology 2020Quote: cDNA was synthesized by using the AffinityScript One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, US) using extracted RNA ...
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bioRxiv - Biochemistry 2020Quote: Point mutations in ABCB1 were introduced into 35S:ABCB1-YFP (26) and 35S:ABCB14-GFP (23) using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... and AlixΔPGY (AlixΔALG-2) cDNAs in pCI were generated by mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) and Alix R757E (AlixΔendo ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting cDNA presented a fragment size distribution of 1,500 up to 5,000 bp on the Bioanalyzer High Sensitivity DNA Kit (Agilent) and a concentration above 300 pg/µl measured with Qubit dsDNA HS fluorometric quantification (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: APP-mCherry was mutated at single or double sites using the QuickChange II Site directed mutagenesis kit (Agilent) following provider-issued recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The RNAt was extracted with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Aliquots of RNAt per each sample were separated and their concentration adjusted at approximately 50 ng/μL to estimate viral accumulation by RT-qPCR (see below).
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bioRxiv - Cancer Biology 2021Quote: The library was prepared with 200 ng of high-quality DNA using the SureSelectXT Library Prep Kit (Agilent). A SureSelectXT Human All Exon V6 kit (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were quantified by quantitative RT-PCR using Agilent qPCR Library Quantification Kit and a MX3005P instrument (Agilent) and relative volumes were pooled accordingly ...
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bioRxiv - Physiology 2021Quote: ... The processed libraries were assessed for its size distribution and concentration using Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ECAR was measured using the XF glycolysis stress test kit according to the manufacturer’s instructions (Seahorse Bioscience, Agilent).
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bioRxiv - Biochemistry 2021Quote: ... and Leu 305 in PP6C were deleted by inserting a stop codon using Quick Change Lightning Kit (Agilent) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and the size distribution was confirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies #5067-4626). Libraries were sequenced on an Illumina HiSeq2500 in single read mode with the read length of 50 nt and sequencing depth of 20 million reads per library following the manufacturer's instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole-exome sequencing library preparation was performed by Admera Health using SureSelect XT Mouse All Exon Kit (Agilent).
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bioRxiv - Developmental Biology 2021Quote: ... Exome capture was undertaken in both affected individuals using the SureSelect 50 Mb All Exon Kit v3 (Agilent) followed by sequencing with a HiSeq2000 (Illumina) ...
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bioRxiv - Neuroscience 2021Quote: ... All RNA samples quality and quantity were determined using RNA Nano kit (2100 Agilent Bioanalyzer Technologies, CA, USA). Only samples with an RNA integrity number greater than 8.0 were used for gene expression analysis.
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bioRxiv - Genetics 2021Quote: ... cleanup and quantified using Qubit (Thermo #Q32851) and Agilent High Sensitivity DNA kit for BioAnalyzer (Agilent #5067-4626). Libraries were sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Genetics 2019Quote: ... The Cdc42 mutants (R186C, R186S, R186L or C188S) were generated by site-directed mutagenesis (Quickchange kit, Agilent Technologies).
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bioRxiv - Biophysics 2020Quote: ... Single mutations in hCx26 and hCx30 were produced using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Genomics 2020Quote: ... 1 - 2 ng / µl was examined using a 2100 Bioanalyzer and a corresponding High Sensitivity DNA Kit (Agilent) to determine the MW profile of the size-selected library ...
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bioRxiv - Microbiology 2020Quote: ... and p-dsbS(S239A)- flag were obtained using the QuikChange II site-directed mutagenesis kit (Stratagene, catalog#: 200518). For generating p-dsbR(D51A)-flag ...
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bioRxiv - Microbiology 2020Quote: ... the primer pair dsbS(H235A)-F/R and a QuikChange II site-directed mutagenesis kit (Stratagene, catalog#:200518) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Point mutations were introduced on that TopoTA-EME1 vector by using a Multiprime site-directed mutagenesis kit (Stratagene). Mutations were confirmed by DNA sequencing ...