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Citations for Agilent :
4551 - 4600 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Mutant versions of JMJD6 were generated from the wild type construct using QuikChange Lightning site-directed mutagenesis kit (Agilent). Amino and carboxy terminal truncations of JMJD6 were generated by PCR amplification ...
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bioRxiv - Cancer Biology 2019Quote: ... and sonicated with the Covaris M220 sonicator or the Diagenode Bioruptor sonicator to obtain chromatin fragment lengths of 100-to-1,000 bp judged by Bioanalyzer DNA High sensitivity kit (Agilent). Fragmented chromatin was diluted in IP buffer (20 mM Tris-HCl pH 8.1 ...
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bioRxiv - Plant Biology 2019Quote: ... Extracts were derivatized using an AccQ•Tag Ultra Derivatization Kit (Waters Corp.) according to the manufacturer’s protocol and analyzed using an HPLC (Agilent) coupled to a mass spectrometer (4500 QTRAP (Sciex) ...
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bioRxiv - Developmental Biology 2019Quote: ... Arg-177-Ala mutants were generated in pCR II-TOPO TA vector using QuikChange site directed mutagenesis kit (Agilent). The wild type and the respective modifications were confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... the integrity and concentration of RNA was confirmed using the RNA 6000 Nano kit on a Bioanalyzer 2100 (Agilent), with reported RNA Integrity Numbers (RIN ...
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bioRxiv - Biophysics 2020Quote: ... Alanine substitution at the 511 tyrosine was made by site-directed mutagenesis using the QuikChange mutagenesis kit (Agilent Technologies).
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bioRxiv - Plant Biology 2020Quote: ... We extracted total RNA from all samples using the Agilent Plant RNA Isolation Mini Kit (Agilent, Santa Clara, Ca), optimized for non-standard plant tissues ...
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bioRxiv - Developmental Biology 2021Quote: ... Final libraries were quantified and checked for fragment size distribution using a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies). Pooled sequencing of Nextera libraries was carried out using a HiSeq4000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... The final product was assessed for its size distribution and concentration using BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). Pooled libraries were diluted to 2 nM in EB buffer (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Mutants were generated with site-directed mutagenesis using synthetic oligonucleotides and Quick Change II XL mutagenesis kit (Agilent Technologies). An additional human Ntrk2 fusion construct (SQSTM1 fused to TrkB kinase (Stransky et al. ...
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bioRxiv - Microbiology 2021Quote: ... RNA integrity was checked by capillary electrophoresis on a 2100 bioanalyzer system using RNA 6000 nano kits (Agilent Technologies). Reverse transcription reactions were carried out with Transcription First-Strand Synthesis kit (Roche AG ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were cleaned up with SPRIselect beads and quantified using Qubit dsDNA HS assay kit (Agilent Technologies). The libraries were sequenced on a HiSeq2500 with paired-end 50-bp reads (Illumina).
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bioRxiv - Systems Biology 2020Quote: ... Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... and quality of the isolated RNA was checked by running RNA 6000 Pico Kit using Agilent 2100 Bioanalyzer (Agilent). Paired-end ...
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bioRxiv - Neuroscience 2020Quote: ... RNA quality was evaluated using an Agilent Bioanalyzer 2100 with an RNA 6000 Pico Kit (Agilent; Cat#5067-1513). The RNA was then converted into cDNA and prepared into RNA-seq libraries using the SmarterStranded kit (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... The average length of final libraries was quantified using a Fragment Analyzer (HS NGS Fragment kit, Agilent, DNF-474) and the concentration of libraries was determined using a Qubit 1X dsDNA HS kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA quality and quantity were assessed with the 2100 Bioanalyzer system using RNA 6000 Pico kit (Agilent Technologies, France) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... G553I) variants were created using the QuikChange II Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Agilent Technologies, #200523). All plasmids were sequenced to confirm their identity (Genewiz).
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bioRxiv - Developmental Biology 2020Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit on a Bioanalyzer 2100 system (Agilent Technologies, CA). Two samples with high RNA integrity numbers (RINs ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The K842M mutation was introduced via Site-Directed Mutagenesis using a QuikChange II Kit (Agilent, Santa Clara, CA, USA).
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bioRxiv - Biochemistry 2021Quote: ... Two variants were created by site-directed mutagenesis using a QuikChange site-directed mutagenesis kit (Stratagene, San Diego, CA) with the primer pairs displayed in Supplementary Table 3 ...
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bioRxiv - Biochemistry 2020Quote: ... The LaGH13_31B Y295A variant was generated using primers in Table S3 and the QuickChange II Site-Directed Mutagenesis kit (Agilent) with pET-28a(+)-LaGH13_31B as template ...
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bioRxiv - Biochemistry 2020Quote: MRG15 W53A was generated by site-directed mutagenesis using an Agilent Quikchange XL Site-Directed Mutagenesis kit (Agilent #200517) with the MRG15 WT in pGEX-6P-3 vector ...
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bioRxiv - Neuroscience 2021Quote: ... and RNA quality assayed using an Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit (Agilent, Santa Clara, CA). First-strand cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... was generated by site directed mutagenesis using primers oM18_17 and oM18_18 (Table 1) and a Quikchange II Site-directed Mutagenesis kit (Agilent, 200523). The PCR product was digested with DpnI enzyme to remove template vector and transformed into Stbl2 chemically competent cells (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... The quality of the DNA libraries was tested with the High Sensitivity DNA Bioanalysis Kit (Agilent, catalog # 5067-4626). DNA concentration of each library was measured with the Qubit dsDNA HS Assay Kit & fluorometer (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and cDNA library size was checked using Fragment Analyzer HS NGS Fragment Kit (1-6000bp) (Agilent formerly Advanced Analytical). Only high-quality cDNA libraries without notable degradation were used for sequencing library construction ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... and quality of the isolated RNA was checked by running RNA 6000 Pico Kit using Agilent 2100 Bioanalyzer (Agilent). Paired-end ...
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bioRxiv - Microbiology 2020Quote: cDNA was synthesized by using Affinity Script One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA). RNA was mixed with random nonamer primers ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid with smc3-K112,113R (AMp1392) was generated by site-directed mutagenesis (using QuikChange XL Site-directed mutagenesis kit, Agilent). Both plasmids were integrated into the LEU2 locus in the pCLB2-3HA-SMC3 strain background.
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bioRxiv - Microbiology 2021Quote: ... Sample quality control was performed using the Bioanalyzer High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent). All samples were prepared and sequenced in technical replicate ...
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bioRxiv - Microbiology 2020Quote: ... Paired-end libraries (∼140-480bp insert size) were prepared using NEXTFlex DNA sequencing kit and quantified with Bioanalyzer (Agilent). WGS was performed using Illumina NextSeq 500 platform and the raw data of each strain were obtained through de-multiplexing.
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bioRxiv - Cancer Biology 2020Quote: ... Quality and quantity of the libraries were assessed with the DNA-7500 kit on a 2100 Bioanalyzer (Agilent Technologies) using a Kapa Library Quantification kit (Kapa Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted from approximately 25 mg of liver tissue using the RecoverEase DNA Isolation Kit (Agilent Technologies). The λ-EG10 phage were packaged in vitro from genomic DNA using Transpack packaging extract (Agilent Technologies) ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Subsequently we strictly adhered to the Seahorse XF Cell Mito Stress Test Kit protocol (Agilent Technologies Cat# 103015-100). Cells were counted before and after the assay using a Celigo S Image cytometer (Nexcelom Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... The final sequencing libraries were inspected and quantified using the Bioanalyzer 2100 Instrument and DNA HS kit (Agilent Technologies). All libraries were diluted to 100 pM working solutions and then pooled as needed to perform the template preparation on an Ion OneTouch 2 according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... The Ribo-Zero treated RNA was quantified by using Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, CA, USA) and further used for the cDNA synthesis by using ScriptSeq™ v2 RNA-Seq kit (Epicentre ...
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bioRxiv - Microbiology 2021Quote: ... Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... EV small RNA size profiles were analyzed using capillary electrophoresis by RNA 6000 Pico Kit (Agilent Technologies 5067-1513) on a Fragment Analyzer (Advanced Analytical) ...
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bioRxiv - Cell Biology 2020Quote: ... The final product was assessed for its size distribution and concentration using BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and pENTR4-HA-TRF2 (S119A/M122A) were produced by oligonucleotide-directed mutagenesis (Quick Change Site-directed Mutagenesis Kit; Stratagene) with the following mutagenic oligonucleotides and their complement.
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bioRxiv - Cell Biology 2021Quote: Yeast two-hybrid (Y2H) screens were performed using the HybriZAP two-hybrid cDNA synthesis kit (Stratagene, La Jolla, USA) as previously described (Maerker et al ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality and quantity was assessed using the Bioanalyzer RNA 6000 Nano Kit (Art. No. 5067-1511, Agilent Technologies) and Qubit™ RNA BR Assay Kit (Art ...
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bioRxiv - Cell Biology 2021Quote: ... Specific point mutations of RanGAP1 were introduced by site-directed mutagenesis with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
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bioRxiv - Cell Biology 2021Quote: ... the pUASt-ifc was used as a backbone and site-directed mutagenesis was done with QuikChange Kit (Agilent 200523) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... The average size and concentration of all sequencing libraries was analysed using a Bioanalyser High Sensitivity DNA Kit (Agilent) followed by qPCR using SensiMix SYBR Green mastermix (Bioline ...
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bioRxiv - Biochemistry 2022Quote: PMCA1d and PMCA2w/a constructs were subjected to site-directed mutagenesis using QuikChange site-directed mutagenesis kit according to the manufacturer’s protocol (Agilent). To obtain an inactive variant D475N (the autophosphorylation site ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was measured directly after mixing 10µl of cellular extract with 50 µl of Luciferase substrate (Luciferase assay kit, Stratagene). Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II ...